Phenotypes associated with this allele

• Allele Symbol: Adamts18^{tm1(KOMP)Vlcg}
• Allele Name: targeted mutation 1, Velocigene
• Allele ID: MGI:4452851
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Adamts18^tm1(KOMP)Vlcg/Adamts18^tm1(KOMP)Vlcg	involves: C57BL/6NTac	MGI:6437247
cx2	Adamts16^em1Amc/Adamts16^em1Amc Adamts18^tm1(KOMP)Vlcg/Adamts18^tm1(KOMP)Vlcg	involves: C57BL/6NTac	MGI:6377458

Genotype
MGI:6437247

hm1

• Allelic Composition: Adamts18^{tm1(KOMP)Vlcg}/Adamts18^{tm1(KOMP)Vlcg}
• Genetic Background: involves: C57BL/6NTac

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

growth/size/body

enlarged kidney ( J:280280 )

• enlarged kidney by E15.5 due to duplicated ureteric outgrowth

renal/urinary system

enlarged kidney ( J:280280 )

• enlarged kidney by E15.5 due to duplicated ureteric outgrowth

abnormal kidney development ( J:280280 )

• 12.5% of E10.5 embryos exhibit structural irregularities in the developing kidney

• kidneys develop double ureters, showing an additional ureteric bud outgrowth at E11.5

• however, subsequent branching of the ureteric bud is unaffected and cell proliferation and apoptosis within the tip populations of kidneys at E12.5 are comparable to wild-type

abnormal nephron number ( J:280280 )

• adult kidneys with duplicated ureters show an increase in nephron numbers

double ureter ( J:280280 )

• 29% of kidneys show duplicated, unilateral ureteric outgrowths arising from the nephric duct and 8% of kidneys show bilateral duplications at E11.5-E15.5

• 70% of double ureter occur in the left kidney

respiratory system

abnormal lung development ( J:280280 )

• marker analysis indicates a delay in the appearance of specialized cell types arising in both proximal (mucosal and ciliated cells) and distal (alveolar epithelial cells) positions of developing airways

• however, cell proliferation and apoptosis within the tip populations of lungs at E12.5 are comparable to wild-type

abnormal branching involved in lung morphogenesis ( J:280280 )

• the primary branch network for all 5 lobes of the lungs is reduced at E12.5, indicating a reduction in primary branch growth

• total epithelial length, total epithelial volume, and tip numbers are smaller in E12.5 lungs

• fewer tip number in E12.5 lungs, indicating fewer branching events

• the primary branches of each lobe maintain the general branch pattern for the secondary lateral branches, however some branches are absent, suggesting a delay in the initial formation of the secondary branches

abnormal lung lobe morphology ( J:280280 )

• E15.5 lobes are misshapen

abnormal right lung accessory lobe morphology ( J:280280 )

• the accessory lobe is shorter and curled at E12.5, then curves back on itself by E13.5

small lung ( J:280280 )

• lungs grow significantly by E15.5 but remain undersized and adult lungs are smaller

• smaller size of lungs is due to shorter primary branches and reduced secondary branching

abnormal lung volume ( J:280280 )

• expansion of lung volumes at E13.5, reflecting the increased branch formation

Genotype
MGI:6377458

cx2

• Allelic Composition: Adamts16^em1Amc/Adamts16^em1Amc; Adamts18^{tm1(KOMP)Vlcg}/Adamts18^{tm1(KOMP)Vlcg}
• Genetic Background: involves: C57BL/6NTac
• Cell Lines: 12442A-F3

renal/urinary system

double ureter ( J:280280 )

• 35% of mice exhibit double ureters, with 4% having bilateral double ureter

• position of secondary ureter formation varies

• kidneys with double ureters have more total epithelial length, total epithelial volume, and total tip numbers compared to single ureters in wild-type mice but to a similar level as seen in single Adam18 homozygotes

• 73% of double ureter occur in the left kidney

respiratory system

abnormal lung development ( J:280280 )

• lungs resemble the lungs of Adam18 homozygotes