Phenotypes associated with this allele

• Allele Symbol: Ikbkb^tm2.1Mka
• Allele Name: targeted mutation 2.1, Michael Karin
• Allele ID: MGI:4453210
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Ikbkb^tm2Mka/Ikbkb^tm2.1Mka Tg(Vil-cre)1Mka/0	involves: 129	MGI:3038976
cn2	Ikbkb^tm2Mka/Ikbkb^tm2.1Mka Tg(Vil-cre)1Mka/0	involves: 129/Sv * C57BL/6	MGI:2662291

Genotype
MGI:3038976

cn1

• Allelic Composition: Ikbkb^tm2Mka/Ikbkb^tm2.1Mka; Tg(Vil-cre)1Mka/0
• Genetic Background: involves: 129

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cellular

increased cellular sensitivity to gamma-irradiation ( J:88644 )

• small intestinal crypt epithelial cells are more sensitive to gamma-radiation, showing an increase in apoptosis after irradiation

increased enterocyte apoptosis ( J:88644 )

• LPS injection alone induces apoptosis in intestinal epithelial cells of mutants but not controls

• small intestinal crypt epithelial cells exhibit an increase in apoptosis after gamma-irradiation

• LPS injection before ionizing radiation significantly increases the number of apoptotic intestinal epithelial cells in mutants by about 70%

digestive/alimentary system

increased enterocyte apoptosis ( J:88644 )

• LPS injection alone induces apoptosis in intestinal epithelial cells of mutants but not controls

• small intestinal crypt epithelial cells exhibit an increase in apoptosis after gamma-irradiation

• LPS injection before ionizing radiation significantly increases the number of apoptotic intestinal epithelial cells in mutants by about 70%

Genotype
MGI:2662291

cn2

• Allelic Composition: Ikbkb^tm2Mka/Ikbkb^tm2.1Mka; Tg(Vil-cre)1Mka/0
• Genetic Background: involves: 129/Sv * C57BL/6

digestive/alimentary system

abnormal intestinal mucosa morphology ( J:83313 )

• loss of mucosal integrity and increased number of apoptotic cells after gut ischemia-reperfusion

intestinal edema ( J:83313 )

• mutants develop severe intestinal edematous change in response to gut ischemia-reperfusion compared to only mild edema in controls

immune system

impaired neutrophil recruitment ( J:83313 )

• neutrophil migration/recruitment into the lung after gut ischemia-reperfusion is reduced and lung edema is prevented

decreased circulating tumor necrosis factor level ( J:83313 )

• the increase in serum TNF-alpha that is observed in controls at 4 hours after gut ischemia-reperfusion is abolished

decreased acute inflammation ( J:83313 )

• reduction in systemic inflammatory response following gut ischemia-reperfusion

homeostasis/metabolism

intestinal edema ( J:83313 )

• mutants develop severe intestinal edematous change in response to gut ischemia-reperfusion compared to only mild edema in controls

decreased circulating tumor necrosis factor level ( J:83313 )

• the increase in serum TNF-alpha that is observed in controls at 4 hours after gut ischemia-reperfusion is abolished

abnormal response to injury ( J:83313 )

• mutants show increased local injury but prevention of systemic inflammation following intestinal ischemia-reperfusion

hematopoietic system

impaired neutrophil recruitment ( J:83313 )

• neutrophil migration/recruitment into the lung after gut ischemia-reperfusion is reduced and lung edema is prevented