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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Cd226tm1.1Cln
targeted mutation 1.1, Marco Colonna
MGI:4454325
Summary 2 genotypes


Genotype
MGI:4454326
hm1
Allelic
Composition		 Cd226tm1.1Cln/Cd226tm1.1Cln
Genetic
Background		 B6.129P2-Cd226tm1.1Cln
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd226tm1.1Cln mutation (0 available); any Cd226 mutation (34 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
cellular
decreased T cell proliferation ( J:159749 )
• decrease in the percentage of dividing CD8 T cells in response to stimulation by staphylococcal enterotoxin B pulsed A20 B cells or A20 B cells transfected with murine Pvr

hematopoietic system
decreased T cell proliferation ( J:159749 )
• decrease in the percentage of dividing CD8 T cells in response to stimulation by staphylococcal enterotoxin B pulsed A20 B cells or A20 B cells transfected with murine Pvr
impaired natural killer cell mediated cytotoxicity ( J:159749 )
• NK cells do not appreciably kill B16F10 tumor cells
• however, the ability to kill YAC-1 cells is unimpaired

immune system
decreased T cell proliferation ( J:159749 )
• decrease in the percentage of dividing CD8 T cells in response to stimulation by staphylococcal enterotoxin B pulsed A20 B cells or A20 B cells transfected with murine Pvr
impaired natural killer cell mediated cytotoxicity ( J:159749 )
• NK cells do not appreciably kill B16F10 tumor cells
• however, the ability to kill YAC-1 cells is unimpaired
decreased inflammatory response ( J:209990 )
• significantly increased survival rates after LPS-induced endotoxicosis at 0.5 or 0.75 mg LPS/30 g levels

neoplasm
increased metastatic potential ( J:159749 )
• with lower tumor doses mice have significantly higher numbers of B16F10 lung metastases than do wild-type
• the injection of the mouse prostate carcinoma, RM-1, at all tumor doses inoculated, results in significantly higher numbers of RM-1 lung metastases than in wild-type
increased tumor growth/size ( J:159749 )
• accelerated tumor growth is observed after subcutaneous injection of B16F10 compared to wild-type
• unlike wild-type mice, mice fail to reject the subcutaneous injection of a colon carcinoma cell line, MC38,that has been transfected with OVA
abnormal tumor susceptibility ( J:209990 )
• more lung metastases after injection of B16F10 melanoma cells into tail vein




Genotype
MGI:4454328
cx2
Allelic
Composition		 Cd226tm1.1Cln/Cd226tm1.1Cln
Tg(TcraTcrb)1100Mjb/0
Genetic
Background		 B6.Cg-Cd226tm1.1Cln Tg(TcraTcrb)1100Mjb
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd226tm1.1Cln mutation (0 available); any Cd226 mutation (34 available)
Tg(TcraTcrb)1100Mjb mutation (6 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
hematopoietic system
decreased cytotoxic T cell cytolysis ( J:159749 )
• CD8 T cells kill B16 cells pulsed with OVA peptide 257-264 less effectively than control CD8 T cells
abnormal T cell activation ( J:159749 )
• CD8 T cells stimulated with T cell lymphoma cell line EL-4 transfected with Pvr and pulsed with OVA peptide 257-264 fail to become activated
decreased T cell proliferation ( J:159749 )
• in CD8 T cells stimulated with T cell lymphoma cell line EL-4 transfected with murine Pvr and pulsed with OVA peptide 257 ? 264
• however, proliferation in response to dendritic cell stimulation is similar to wild-type

immune system
decreased cytotoxic T cell cytolysis ( J:159749 )
• CD8 T cells kill B16 cells pulsed with OVA peptide 257-264 less effectively than control CD8 T cells
abnormal T cell activation ( J:159749 )
• CD8 T cells stimulated with T cell lymphoma cell line EL-4 transfected with Pvr and pulsed with OVA peptide 257-264 fail to become activated
decreased T cell proliferation ( J:159749 )
• in CD8 T cells stimulated with T cell lymphoma cell line EL-4 transfected with murine Pvr and pulsed with OVA peptide 257 ? 264
• however, proliferation in response to dendritic cell stimulation is similar to wild-type
decreased interferon-gamma secretion ( J:159749 )
• fewer IFN gamma secreting T cells are evident in lymph nodes after restimulation with the T cell lymphoma cell line EL-4 cells transfected with Pvr and pulsed with OVA peptide 257-264
• however, the frequency of IFN gamma secreting cells is similar to wild-type after restimulation with dendritic cells

cellular
decreased T cell proliferation ( J:159749 )
• in CD8 T cells stimulated with T cell lymphoma cell line EL-4 transfected with murine Pvr and pulsed with OVA peptide 257 ? 264
• however, proliferation in response to dendritic cell stimulation is similar to wild-type




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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory