About   Help   FAQ
Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Appl1tm1.2Test
targeted mutation 1.2, Joseph Testa
MGI:4454678
Summary 3 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Appl1tm1.2Test/Appl1tm1.2Test involves: 129X1/SvJ MGI:4838333
hm2
 		Appl1tm1.2Test/Appl1tm1.2Test involves: 129X1/SvJ * C57BL/6 MGI:4454680
cx3
 		Appl1tm1.2Test/Appl1tm1.2Test
Appl2tm1.1Test/Appl2tm1.1Test 		involves: 129X1/SvJ MGI:5766636


Genotype
MGI:4838333
hm1
Allelic
Composition		 Appl1tm1.2Test/Appl1tm1.2Test
Genetic
Background		 involves: 129X1/SvJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Appl1tm1.2Test mutation (0 available); any Appl1 mutation (47 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
hematopoietic system
hematopoietic system phenotype ( J:227340 )
N
• at 8 weeks of age, mice exhibit normal numbers and % of lymphocytes, monocytes, neutrophils, eosinophils, and basophils relative to controls
• no pernicious anemia is observed, as WBC counts and spleen and liver size are normal
increased T cell proliferation ( J:164696 )
• slightly in response to ConA treatment
increased hematocrit ( J:227340 )
• hematocrit is slightly but significantly increased, esp. in female mice

cellular
abnormal cell physiology ( J:227340 )
• primary MEFs derived from E13.5 embryos exhibit decreased Akt activation upon stimulation with HGF
• however, in vivo, basal Akt activity is not significantly altered in stomach, liver or muscle tissues
decreased fibroblast cell migration ( J:227340 )
• primary MEFs derived from E13.5 embryos exhibit reduced HGF-induced transmembrane migration and decreased movement in a wound-healing assay as well as markedly decreased HGF-triggered invasion in a matrigel assay
• in contrast, EGF-induced migration and invasion are normal
increased T cell proliferation ( J:164696 )
• slightly in response to ConA treatment

immune system
increased T cell proliferation ( J:164696 )
• slightly in response to ConA treatment




Genotype
MGI:4454680
hm2
Allelic
Composition		 Appl1tm1.2Test/Appl1tm1.2Test
Genetic
Background		 involves: 129X1/SvJ * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Appl1tm1.2Test mutation (0 available); any Appl1 mutation (47 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
cellular
cellular phenotype ( J:159889 )
N
• mouse embryonic fibroblasts exhibit normal proliferation, survival under normal conditions, and UV-induced apoptosis
increased sensitivity to induced cell death ( J:159889 )
• mouse embryonic fibroblast cells cultured in the presence of HGF exhibit impaired survival compared with similarly treated wild-type cells
decreased fibroblast cell migration ( J:159889 )
• mouse embryonic fibroblasts exhibit reduced HGF-induced migration compared with similarly treated wild-type cells

hematopoietic system
hematopoietic system phenotype ( J:159889 )
N
• mice exhibit no obvious hematopoietic system defects




Genotype
MGI:5766636
cx3
Allelic
Composition		 Appl1tm1.2Test/Appl1tm1.2Test
Appl2tm1.1Test/Appl2tm1.1Test
Genetic
Background		 involves: 129X1/SvJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Appl1tm1.2Test mutation (0 available); any Appl1 mutation (47 available)
Appl2tm1.1Test mutation (0 available); any Appl2 mutation (27 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
mortality/aging ( J:227340 )
N
• unexpectedly, mice are viable and show grossly normal postnatal growth and fertility, similar to single homozygotes

hematopoietic system
hematopoietic system phenotype ( J:227340 )
N
• at 8 weeks of age, mice exhibit normal numbers and % of lymphocytes, monocytes, neutrophils, eosinophils, and basophils relative to controls
• no pernicious anemia is observed, as WBC counts and spleen and liver size are normal
• T cell development and Pi3k-Akt signaling in thymic T cells are normal
increased hematocrit ( J:227340 )
• hematocrit is significantly increased, esp. in female mice
decreased mean corpuscular hemoglobin concentration ( J:227340 )
• MCHC is significantly decreased, esp. in female mice
increased mean corpuscular volume ( J:227340 )
• MCV is significantly increased, esp. in female mice

cellular
abnormal cell physiology ( J:227340 )
• primary MEFs derived from E13.5 embryos exhibit decreased Akt activation upon stimulation with HGF; in contrast, FBS- or EGF-induced Akt activation is relatively normal
• in vivo, basal Akt activity is not significantly altered in stomach, liver or muscle tissues
decreased fibroblast cell migration ( J:227340 )
• primary MEFs derived from E13.5 embryos exhibit reduced HGF-induced transmembrane migration and decreased movement in a wound-healing assay as well as markedly decreased HGF-triggered invasion in a matrigel assay
• in contrast, EGF-induced migration and invasion are normal




Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory