Phenotypes associated with this allele

• Allele Symbol: Tg(Runx2-icre)1Jtuc
• Allele Name: transgene insertion 1, Jan Tuckermann
• Allele ID: MGI:4455015
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Nr3c1^tm2Gsc/Nr3c1^tm2Gsc Tg(Runx2-icre)1Jtuc/0	involves: 129P2/OlaHsd * FVB/N	MGI:4455043
cn2	Ctnnb1^tm2(Nfkbia)Rsu/Ctnnb1^tm2(Nfkbia)Rsu Tg(Runx2-icre)1Jtuc/0	involves: 129P2/OlaHsd * FVB/N	MGI:4455056
cn3	Gt(ROSA)26Sor^tm1(CAG-Slc39a14*L438R)Wvh/Gt(ROSA)26Sor^+ Tg(Runx2-icre)1Jtuc/0	involves: FVB/N	MGI:6159136

Genotype
MGI:4455043

cn1

• Allelic Composition: Nr3c1^tm2Gsc/Nr3c1^tm2Gsc; Tg(Runx2-icre)1Jtuc/0
• Genetic Background: involves: 129P2/OlaHsd * FVB/N

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

growth/size/body

increased body length ( J:160669 )

• males exhibit a slight increase in crown-rump length compared to controls at 10 weeks

skeleton

skeleton phenotype ( J:160669 )

N • bone resorption is slightly decreased in both mutants and controls while osteoclast numbers are not affected

abnormal osteoblast differentiation ( J:160669 )

• calvarial osteoblasts exhibit diminished differentiation potential in terms of bone nodule formation and alkaline phosphatase expression

• with prednisolone treatment, osteoblast differentiation is impaired in wild-type mice, but is unaffected in mutants, as determined by Col1a1 expression, marking functional osteoblasts

abnormal bone mineral density ( J:160669 )

• in response to two weeks of treatment with the Nr3c1 agonist prednisolone, mutants do not display reduced bone mineral density in vertebral bones in contrast to treated controls

decreased bone mineral density ( J:160669 )

• mice show modest but highly significant decrease in bone density in calcified vertebral sections compared to controls at 3 months

decreased bone trabecula number ( J:160669 )

increased bone trabecular spacing ( J:160669 )

• trabecular numbers are decreased but trabecular thickness is unaltered resulting in increased trabecular spacing

• osteoblast numbers, osteoblast surface, osteocytes, and osteoclast parameters are not significantly changed compared to controls

abnormal bone ossification ( J:160669 )

• bone formation in vertebrae is not inhibited whereas treated controls show near complete inhibition of bone formation with prednisolone treatment; osteoblastogenesis (numbers of colony forming units of osteoblasts) is not impaired as seen in controls with prednisolone treatment

cellular

abnormal osteoblast differentiation ( J:160669 )

• calvarial osteoblasts exhibit diminished differentiation potential in terms of bone nodule formation and alkaline phosphatase expression

• with prednisolone treatment, osteoblast differentiation is impaired in wild-type mice, but is unaffected in mutants, as determined by Col1a1 expression, marking functional osteoblasts

Genotype
MGI:4455056

cn2

• Allelic Composition: Ctnnb1^{tm2(Nfkbia)Rsu}/Ctnnb1^{tm2(Nfkbia)Rsu}; Tg(Runx2-icre)1Jtuc/0
• Genetic Background: involves: 129P2/OlaHsd * FVB/N

skeleton

skeleton phenotype ( J:160669 )

N • osteoblast apoptosis is normal and differentiation is inhibited by dexamethasone treatment, similar to wild-type

Genotype
MGI:6159136

cn3

• Allelic Composition: Gt(ROSA)26Sor^{tm1(CAG-Slc39a14*L438R)Wvh}/Gt(ROSA)26Sor⁺; Tg(Runx2-icre)1Jtuc/0
• Genetic Background: involves: FVB/N

skeleton

abnormal osteoblast physiology ( J:261326 )

♂ • long bone osteoblasts exhibit an increased expression of inflammatory cytokines during osteoblast differentiation

increased osteoclast cell number ( J:261326 )

• osteoclast number is increased in lumbar vertebral bodies and tibiae and osteoclast-covered surface of lumbar vertebral bodies and tibiae is increased in males

• osteoclast surface per bone surface and osteoclast number per bone perimeter is increased in vertebral bodies of females

• however, no differences in osteoblast-covered surface or number are seen

decreased diameter of femur ( J:261326 )

• femurs exhibit a smaller midshaft diameter

decreased diameter of tibia ( J:261326 )

♀ • tibia show decreased midshaft diameter

decreased trabecular bone volume ( J:261326 )

• decrease in trabecular bone volume in femurs and lumbar spine of males

• decrease in trabecular bone volume in lumbar spine of females

abnormal compact bone morphology ( J:261326 )

♂ • femurs show decreased cortical porosity

increased femur compact bone thickness ( J:261326 )

• femurs and tibiae show an increase in cortical thickness

decreased bone trabecula number ( J:261326 )

• decrease in trabecula number in femurs and lumbar spine of males

• decrease in trabecula number in lumbar spine of females

increased bone trabecular spacing ( J:261326 )

♂ • increase in trabecular separation in femurs and lumbar spine

decreased trabecular bone connectivity density ( J:261326 )

♂ • decrease in trabecular bone connection density

decreased trabecular bone mass ( J:261326 )

• femurs show decreased trabecular mass

decreased bone mineralization ( J:261326 )

♂ • reduction in cortical mineralization of femurs

increased bone mineralization ( J:261326 )

• tibial endocortical bone surface shows an increase in endosteal mineralizing surface

increased bone ossification ( J:261326 )

♂ • tibial endocortical bone surface shows an increase in endosteal bone formation rate

♂ • however, no differences in periosteal or trabecular bone formation parameters

decreased femur stiffness ( J:261326 )

♂ • the elastic modulus and work to reach ultimate stress levels are lower in femurs, indicating that femurs tolerate higher stress levels and are more elastic/flexible

fragile skeleton ( J:261326 )

♂ • two mice exhibit fracture with callus in the tibia

♂ • femurs bear higher stress levels in the three-point bending tests but work-to-fracture is 42% lower

cellular

abnormal osteoblast physiology ( J:261326 )

♂ • long bone osteoblasts exhibit an increased expression of inflammatory cytokines during osteoblast differentiation

hematopoietic system

increased osteoclast cell number ( J:261326 )

• osteoclast number is increased in lumbar vertebral bodies and tibiae and osteoclast-covered surface of lumbar vertebral bodies and tibiae is increased in males

• osteoclast surface per bone surface and osteoclast number per bone perimeter is increased in vertebral bodies of females

• however, no differences in osteoblast-covered surface or number are seen

immune system

increased osteoclast cell number ( J:261326 )

• osteoclast number is increased in lumbar vertebral bodies and tibiae and osteoclast-covered surface of lumbar vertebral bodies and tibiae is increased in males

• osteoclast surface per bone surface and osteoclast number per bone perimeter is increased in vertebral bodies of females

• however, no differences in osteoblast-covered surface or number are seen

limbs/digits/tail

increased femur compact bone thickness ( J:261326 )

• femurs and tibiae show an increase in cortical thickness

decreased diameter of femur ( J:261326 )

• femurs exhibit a smaller midshaft diameter

decreased diameter of tibia ( J:261326 )

♀ • tibia show decreased midshaft diameter

craniofacial

craniofacial phenotype ( J:261326 )

N • mice exhibit normal calvarial bone thickness and porosity