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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Brdttm1a(EUCOMM)Wtsi
targeted mutation 1a, Wellcome Trust Sanger Institute
MGI:4455954
Summary 3 genotypes


Genotype
MGI:5782037
hm1
Allelic
Composition
Brdttm1a(EUCOMM)Wtsi/Brdttm1a(EUCOMM)Wtsi
Genetic
Background
B6JTyr;B6N-Brdttm1a(EUCOMM)Wtsi/Wtsi
Cell Lines EPD0126_2_D06
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Brdttm1a(EUCOMM)Wtsi mutation (1 available); any Brdt mutation (39 available)
Data Sources
phenotype observed in females
phenotype observed in males
N normal phenotype
skeleton




Genotype
MGI:5790374
hm2
Allelic
Composition
Brdttm1a(EUCOMM)Wtsi/Brdttm1a(EUCOMM)Wtsi
Genetic
Background
C57BL/6-Brdttm1a(EUCOMM)Wtsi
Cell Lines EPD0126_2_D06
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Brdttm1a(EUCOMM)Wtsi mutation (1 available); any Brdt mutation (39 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
reproductive system
• significantly increased number of TUNEL+ apoptotic cells per seminiferous tubule at 8, 14 and 28 weeks of age relative to wild-type controls
• significantly decreased seminal vesicle + coagulating gland weight at 8 weeks but not at 14 or 28 weeks of age relative to wild-type controls
• at 8 weeks of age, all seminiferous tubules display a basal single layer of spermatogonia and contain Sertoli cells along with 2-5 layers of primary spermatocytes
• no mature spermatozoa are found in the lumen of seminiferous tubules at 8 weeks
• cells suspected as being round spermatids are found near the central lumen at 14 and 28 weeks of age
• significantly decreased testis weight at 8, 14 and 28 weeks of age relative to wild-type controls
• disruption of spermatogenesis before the first meiotic division, consistent with presence of pachytene spermatocytes at 8 weeks of age, as revealed by gamma-H2AX labeling
• complete absence of epididymal sperm
• presumed round spermatids are detected only in ~25% of seminiferous tubules at 14 and 28 weeks of age
• round spermatids appear abnormal, with acrosin accumulating throughout the cytoplasm instead of forming a cap-like structure on the nuclear envelope as in wild-type round spermatids
• number of round spermatids per tubule cross-section is reduced relative to wild-type controls
• however, no evidence of any elongating or elongated spermatids is observed
• at 8 weeks of age, some spermatocytes appear to undergo nuclear condensation and pyknosis
• eosinophilic staining of spermatocyte cytoplasm indicates progressive degeneration and apoptosis
• meiotic arrest at the spermatocyte stage at 8 weeks of age
• male homozygotes display delayed puberty, as shown by normal testosterone levels and accessory gland weights at 14 and 28 weeks of age
• continuous mating of male homozygotes with fertile females does not result in pregnancy

endocrine/exocrine glands
• significantly decreased seminal vesicle + coagulating gland weight at 8 weeks but not at 14 or 28 weeks of age relative to wild-type controls
• at 8 weeks of age, all seminiferous tubules display a basal single layer of spermatogonia and contain Sertoli cells along with 2-5 layers of primary spermatocytes
• no mature spermatozoa are found in the lumen of seminiferous tubules at 8 weeks
• cells suspected as being round spermatids are found near the central lumen at 14 and 28 weeks of age
• significantly decreased testis weight at 8, 14 and 28 weeks of age relative to wild-type controls

homeostasis/metabolism
• significantly decreased plasma testosterone levels at 8 weeks but not at 14 or 28 weeks of age relative to wild-type controls
• however, no changes in Leydig cell number and/or size are detected between 8 and 28 weeks of age

cellular
• complete absence of epididymal sperm
• presumed round spermatids are detected only in ~25% of seminiferous tubules at 14 and 28 weeks of age
• round spermatids appear abnormal, with acrosin accumulating throughout the cytoplasm instead of forming a cap-like structure on the nuclear envelope as in wild-type round spermatids
• number of round spermatids per tubule cross-section is reduced relative to wild-type controls
• however, no evidence of any elongating or elongated spermatids is observed
• at 8 weeks of age, some spermatocytes appear to undergo nuclear condensation and pyknosis
• eosinophilic staining of spermatocyte cytoplasm indicates progressive degeneration and apoptosis
• meiotic arrest at the spermatocyte stage at 8 weeks of age
• significantly increased number of TUNEL+ apoptotic cells per seminiferous tubule at 8, 14 and 28 weeks of age relative to wild-type controls




Genotype
MGI:5448640
hm3
Allelic
Composition
Brdttm1a(EUCOMM)Wtsi/Brdttm1a(EUCOMM)Wtsi
Genetic
Background
involves: C57BL/6N
Cell Lines EPD0126_2_D06
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Brdttm1a(EUCOMM)Wtsi mutation (1 available); any Brdt mutation (39 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
reproductive system
• testes lack post-meiotic cells
• meiosis I is not completed
• however, late pachytene stage occurs normally

cellular
• testes lack post-meiotic cells
• meiosis I is not completed
• however, late pachytene stage occurs normally





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last database update
11/19/2024
MGI 6.24
The Jackson Laboratory