Phenotypes associated with this allele

• Allele Symbol: Actb^{tm3.1(Sirt1)Npa}
• Allele Name: targeted mutation 3.1, Novartis Pharma AG
• Allele ID: MGI:4456082
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Actb^tm3.1(Sirt1)Npa/Actb^tm3.1(Sirt1)Npa	involves: 129/Sv * C57BL/6 * C57BL/6J	MGI:4456086
ht2	Actb^tm3.1(Sirt1)Npa/Actb^+	involves: 129/Sv * C57BL/6 * C57BL/6J	MGI:4456085
cx3	Actb^tm3.1(Sirt1)Npa/Actb^+ Sirt1^tm1Mcby/Sirt1^tm1Mcby	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * C57BL/6J	MGI:4456088

Genotype
MGI:4456086

hm1

• Allelic Composition: Actb^{tm3.1(Sirt1)Npa}/Actb^{tm3.1(Sirt1)Npa}
• Genetic Background: involves: 129/Sv * C57BL/6 * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

preweaning lethality, complete penetrance ( J:160212 )

• no mice are produced (no time of death given)

Genotype
MGI:4456085

ht2

• Allelic Composition: Actb^{tm3.1(Sirt1)Npa}/Actb⁺
• Genetic Background: involves: 129/Sv * C57BL/6 * C57BL/6J

mortality/aging

preweaning lethality, incomplete penetrance ( J:160212 )

• fewer than expected mice are produced (no time of death given)

reproductive system

delayed fertility ( J:160212 )

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:160212 )

N • mice exhibit normal levels of circulating triglyceride, glycerol, IGF, IGF-1, lactate, hydrobutyrate, and corticosterone

decreased fasting circulating glucose level ( J:160212 )

• reduced plasma glucose levels in fasted mice at 6 months of age

decreased circulating insulin level ( J:160212 )

• reduced plasma insulin levels in fed mice at 6 months of age

decreased circulating leptin level ( J:160212 )

• at 3 and 12 months

decreased circulating cholesterol level ( J:160212 )

• at 3 and 12 months

decreased circulating HDL cholesterol level ( J:160212 )

decreased circulating LDL cholesterol level ( J:160212 )

decreased circulating free fatty acids level ( J:160212 )

• at 12 months

increased oxygen consumption ( J:160212 )

• at 7 months of age

improved glucose tolerance ( J:160212 )

decreased circulating adiponectin level ( J:160212 )

• at 12 months

behavior/neurological

behavior/neurological phenotype ( J:160212 )

N • mice exhibit normal baseline physical activity

increased food intake ( J:160212 )

• increase in food consumption normalized to body weight

abnormal motor coordination/balance ( J:160212 )

• mice exhibit improved performance on a rotarod compared with wild-type mice

growth/size/body

decreased body weight ( J:160212 )

adipose tissue

decreased epididymal fat pad weight ( J:160212 )

• at 3 months

Genotype
MGI:4456088

cx3

• Allelic Composition: Actb^{tm3.1(Sirt1)Npa}/Actb⁺; Sirt1^tm1Mcby/Sirt1^tm1Mcby
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * C57BL/6J

reproductive system

reproductive system phenotype ( J:160212 )

N • unlike Sirt1^tm1Mcby homozygotes, mice are fertile

growth/size/body

growth/size/body region phenotype ( J:160212 )

N • unlike Sirt1^tm1Mcby homozygotes, mice exhibit normal body size and weight

vision/eye

vision/eye phenotype ( J:160212 )

N • unlike Sirt1^tm1Mcby homozygotes, eyelids open