Phenotypes associated with this allele

• Allele Symbol: Trpa1^tm1.1Bju
• Allele Name: targeted mutation 1.1, Bradley J Undem
• Allele ID: MGI:4458079
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Trpa1^tm1.1Bju/Trpa1^tm1.1Bju	involves: C57BL/6J	MGI:4458086

Genotype
MGI:4458086

hm1

• Allelic Composition: Trpa1^tm1.1Bju/Trpa1^tm1.1Bju
• Genetic Background: involves: C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

abnormal neuron physiology ( J:160860 , J:161343 )

• dissociated trigeminal neurons respond to toluene diisocyanate (TDI) is almost completely abolished compared with similarly treated wild-type neurons (J:160860)

• dissociated trigeminal neurons fail to respond to allyl isothiocyanate (AITC) unlike similarly treated neurons (J:160860)

• however, neurons respond normally to capsaicin (J:160860)

• the selective TRPA1 channel agonist allyl isothiocyanate (AITC) is ineffective in increasing cytosolic calcium in neurons unlike similarly treated wild-type cells (J:161343)

• trigeminal neurons fail to respond to 15-deoxy-delta12,14-prostagladin J2 unlike similarly treated wild-type mice (J:161343)

• no bronchopulmonary C nerve fibre terminals pretreated with ruthenium red respond to 10 um or 100 um 4-oxononenal (4ONE) compared with 2 of 5 similarly treated wild-type fibres (J:161343)

• however, C-fibres respond normally to capsaicin (J:161343)

abnormal sensory neuron physiology ( J:161343 )

• vagal sensory neurons fail to respond to 10 um 4-oxononenal (4ONE) unlike similarly treated wild-type cells

• 63 out of 144 100 um 4ONE-treated neurons exhibit a calcium response unlike similarly treated wild-type cells

• 66 of 151 neurons treated with ruthenium and a pharmacological blockade of TRPV1 respond with a maximal response to 4ONE at all doses compared with 182 of 276 similarly treated wild-type cells

respiratory system

abnormal bronchial provocation ( J:160860 )

• exposure to allyl isothiocyanate (AITC) or 1% toluene diisocyanate (TDI) fails to decrease respiratory rate or increase in time to braking unlike in similarly treated wild-type mice

• the decrease in respiratory rate following exposure to formalin is attenuated compared with similarly treated wild-type mice

• mice exposed with 1% TDI do not exhibit a change in expiratory flow at half tidal volume unlike similarly treated wild-type mice

• however, mice exposed to nicotine exhibit a decrease in respiratory rate as in wild-type mice

homeostasis/metabolism

decreased physiological sensitivity to xenobiotic ( J:160860 )

• exposure to allyl isothiocyanate (AITC) or 1% toluene diisocyanate (TDI) fails to decrease respiratory rate or increase in time to braking unlike in similarly treated wild-type mice

• the decrease in respiratory rate following exposure to formalin is attenuated compared with similarly treated wild-type mice

• mice exposed with 1% TDI do not exhibit a change in expiratory flow at half tidal volume unlike similarly treated wild-type mice

• however, mice exposed to nicotine exhibit a decrease in respiratory rate as in wild-type mice