Phenotypes associated with this allele

• Allele Symbol: Neurog3^tm3.1Ggr
• Allele Name: targeted mutation 3.1, Gerard Gradwohl
• Allele ID: MGI:4460088
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Neurog3^tm3.1Ggr/Neurog3^tm3.1Ggr	involves: 129S2/SvPas * C57BL/6 * CD-1 * SJL	MGI:4460259
cn2	Neurog3^tm1Fgu/Neurog3^tm3.1Ggr Tg(Vil1-cre)20Syr/0	involves: 129S2/SvPas * C57BL/6 * CD-1 * DBA/2 * SJL	MGI:4460260
cn3	Neurog3^tm3.1Ggr/Neurog3^tm3.1Ggr Tg(Vil1-cre)20Syr/0	involves: 129S2/SvPas * C57BL/6 * CD-1 * DBA/2 * SJL	MGI:4460261
cn4	Neurog3^tm3.1Ggr/Neurog3^tm3.1Ggr Tg(CDX2-cre)101Erf/0	involves: 129S2/SvPas * C57BL/6J * SJL/J	MGI:6711505

Genotype
MGI:4460259

hm1

• Allelic Composition: Neurog3^tm3.1Ggr/Neurog3^tm3.1Ggr
• Genetic Background: involves: 129S2/SvPas * C57BL/6 * CD-1 * SJL

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

normal phenotype

no abnormal phenotype detected ( J:161480 )

• mice develop normally, reach adulthood, are fertile, and exhibit normal glucose levels in the urine

Genotype
MGI:4460260

cn2

• Allelic Composition: Neurog3^tm1Fgu/Neurog3^tm3.1Ggr; Tg(Vil1-cre)20Syr/0
• Genetic Background: involves: 129S2/SvPas * C57BL/6 * CD-1 * DBA/2 * SJL

Loss of enteroendocrine cells in Neurog3^tm1Fgu/Neurog3^tm3.1Ggr Tg(Vil1-cre)20Syr/0 and Neurog3^tm3.1Ggr/Neurog3^tm3.1Ggr Tg(Vil1-cre)20Syr/0 mice

mortality/aging

postnatal lethality, incomplete penetrance ( J:161480 )

• 50% of mice die within the first 8 days of life

digestive/alimentary system

abnormal intestinal goblet cell morphology ( J:161480 )

• goblet cells in the large intestine are larger than in wild-type mice and mostly devoid of mucus

• however, goblet cell numbers are normal in the small intestine

abnormal small intestinal crypt cell proliferation ( J:161480 )

• at E19.5, crypt cell proliferation is increased compared to in wild-type mice

• in adult mice, crypt cell proliferation is increased with an up to 1.6-fold increase in cell turnover compared to in wild-type mice

abnormal intestine development ( J:161480 )

• as early as embryogenesis, mice lack enteroendocrine progenitors along the proximal-distal axis of the intestine unlike in wild-type mice

• however, intestinal epithelial cell proliferation is normal

abnormal intestinal enteroendocrine cell morphology ( J:161480 )

• as early as embryogenesis, mice lack enteroendocrine progenitors along the proximal-distal axis of the intestine unlike in wild-type mice

steatorrhea ( J:161480 )

• mice excrete yellowish stool unlike wild-type mice

diarrhea ( J:161480 )

• surviving mice exhibit soft stool that does not cease with age unlike in wild-type mice

abnormal small intestine crypts of Lieberkuhn morphology ( J:161480 )

• small intestine crypts are disorganized, larger, and more abundant than in wild-type mice

abnormal large intestine morphology ( J:161480 )

• the large intestine exhibits a reduction in the length of glands of up to 1.5 times compared with wild-type mice

abnormal small intestinal villus morphology ( J:161480 )

• villi are blunted or club shaped with frequent dilation and strong detachment of the epithelium from the basement membrane unlike in wild-type mice

abnormal small intestinal microvillus morphology ( J:161480 )

• microvilli on absorptive cells are sparser, 60% shorter, but twice as large as on wild-type cells

• the brush border of absorptive cells in the small intestine is reduced 44% compared to in wild-type mice

• however, expression of brush border enzymes and glucose transporters is normal

decreased small intestinal microvillus size ( J:161480 )

• 60% shorter than in wild-type mice

increased defecation amount ( J:161480 )

• mice produce more feces compared with wild-type mice

abnormal intestinal lipid absorption ( J:161480 )

• impaired

abnormal intestinal transit time ( J:161480 )

• intestinal transit time is increased 2.3-fold compared to in wild-type mice

homeostasis/metabolism

steatorrhea ( J:161480 )

• mice excrete yellowish stool unlike wild-type mice

abnormal intestinal lipid absorption ( J:161480 )

• impaired

increased blood urea nitrogen level ( J:161480 )

• slightly

increased circulating bicarbonate level ( J:161480 )

• slightly

decreased circulating glucose level ( J:161480 )

• in an intraperitoneal glucose tolerance test, mice exhibit lower serum glucose levels than in similarly treated wild-type mice

• fasting mice exhibit decreased blood glucose compared with similarly treated wild-type mice

decreased circulating cholesterol level ( J:161480 )

decreased circulating HDL cholesterol level ( J:161480 )

decreased circulating triglyceride level ( J:161480 )

decreased circulating serum albumin level ( J:161480 )

improved glucose tolerance ( J:161480 )

• in an oral glucose tolerance test, mice exhibit improved glucose clearance compared with similarly treated wild-type mice

• in an intraperitoneal glucose tolerance test, mice exhibit lower serum glucose levels than in similarly treated wild-type mice

increased insulin sensitivity ( J:161480 )

• slightly at 14 and 17 weeks

• insulin-mediated hypoglycemic response is blunted compared to in wild-type mice

growth/size/body

decreased birth weight ( J:161480 )

decreased body mass index ( J:161480 )

increased lean body mass ( J:161480 )

decreased body size ( J:161480 )

postnatal growth retardation ( J:161480 )

adipose tissue

decreased total body fat amount ( J:161480 )

abnormal abdominal fat pad morphology ( J:161480 )

• mice exhibit reduced abdominal fat compared with wild-type mice

endocrine/exocrine glands

abnormal small intestine crypts of Lieberkuhn morphology ( J:161480 )

• small intestine crypts are disorganized, larger, and more abundant than in wild-type mice

abnormal pancreatic islet morphology ( J:161480 )

• mice exhibit more medium and large islets with centrally located alpha cells than in wild-type mice

small pancreatic islets ( J:161480 )

• mice exhibit a shift from large to single islets compared with wild-type mice

cellular

abnormal intestinal goblet cell morphology ( J:161480 )

• goblet cells in the large intestine are larger than in wild-type mice and mostly devoid of mucus

• however, goblet cell numbers are normal in the small intestine

abnormal small intestinal crypt cell proliferation ( J:161480 )

• at E19.5, crypt cell proliferation is increased compared to in wild-type mice

• in adult mice, crypt cell proliferation is increased with an up to 1.6-fold increase in cell turnover compared to in wild-type mice

Genotype
MGI:4460261

cn3

• Allelic Composition: Neurog3^tm3.1Ggr/Neurog3^tm3.1Ggr; Tg(Vil1-cre)20Syr/0
• Genetic Background: involves: 129S2/SvPas * C57BL/6 * CD-1 * DBA/2 * SJL

Loss of enteroendocrine cells in Neurog3^tm1Fgu/Neurog3^tm3.1Ggr Tg(Vil1-cre)20Syr/0 and Neurog3^tm3.1Ggr/Neurog3^tm3.1Ggr Tg(Vil1-cre)20Syr/0 mice

mortality/aging

postnatal lethality, incomplete penetrance ( J:161480 )

• 50% of mice die within the first 8 days of life

homeostasis/metabolism

steatorrhea ( J:161480 )

• mice excrete yellowish stool unlike wild-type mice

abnormal intestinal lipid absorption ( J:161480 )

• impaired

increased blood urea nitrogen level ( J:161480 )

• slightly

increased circulating bicarbonate level ( J:161480 )

• slightly

decreased circulating glucose level ( J:161480 )

• in an intraperitoneal glucose tolerance test, mice exhibit lower serum glucose levels than in similarly treated wild-type mice

• fasting mice exhibit decreased blood glucose compared with similarly treated wild-type mice

decreased circulating cholesterol level ( J:161480 )

decreased circulating HDL cholesterol level ( J:161480 )

decreased circulating triglyceride level ( J:161480 )

decreased circulating serum albumin level ( J:161480 )

improved glucose tolerance ( J:161480 )

• in an oral glucose tolerance test, mice exhibit improved glucose clearance compared with similarly treated wild-type mice

• in an intraperitoneal glucose tolerance test, mice exhibit lower serum glucose levels than in similarly treated wild-type mice

increased insulin sensitivity ( J:161480 )

• slightly at 14 and 17 weeks

• insulin-mediated hypoglycemic response is blunted compared to in wild-type mice

digestive/alimentary system

abnormal intestinal goblet cell morphology ( J:161480 )

• goblet cells in the large intestine are larger than in wild-type mice and mostly devoid of mucus

• however, goblet cell numbers are normal in the small intestine

abnormal small intestinal crypt cell proliferation ( J:161480 )

• at E19.5, crypt cell proliferation is increased compared to in wild-type mice

• in adult mice, crypt cell proliferation is increased with an up to 1.6-fold increase in cell turnover compared to in wild-type mice

abnormal intestine development ( J:161480 )

• as early as embryogenesis, mice lack enteroendocrine progenitors along the proximal-distal axis of the intestine unlike in wild-type mice

• however, intestinal epithelial cell proliferation is normal

abnormal intestinal enteroendocrine cell morphology ( J:161480 )

• as early as embryogenesis, mice lack enteroendocrine progenitors along the proximal-distal axis of the intestine unlike in wild-type mice

steatorrhea ( J:161480 )

• mice excrete yellowish stool unlike wild-type mice

diarrhea ( J:161480 )

• surviving mice exhibit soft stool that does not cease with age unlike in wild-type mice

abnormal small intestine crypts of Lieberkuhn morphology ( J:161480 )

• small intestine crypts are disorganized, larger, and more abundant than in wild-type mice

abnormal large intestine morphology ( J:161480 )

• the large intestine exhibits a reduction in the length of glands of up to 1.5 times compared with wild-type mice

abnormal small intestinal villus morphology ( J:161480 )

• villi are blunted or club shaped with frequent dilation and strong detachment of the epithelium from the basement membrane unlike in wild-type mice

abnormal small intestinal microvillus morphology ( J:161480 )

• microvilli on absorptive cells are sparser, 60% shorter, but twice as large as on wild-type cells

• the brush border of absorptive cells in the small intestine is reduced 44% compared to in wild-type mice

• however, expression of brush border enzymes and glucose transporters is normal

decreased small intestinal microvillus size ( J:161480 )

• 60% shorter than in wild-type mice

increased defecation amount ( J:161480 )

• mice produce more feces compared with wild-type mice

abnormal intestinal lipid absorption ( J:161480 )

• impaired

abnormal intestinal transit time ( J:161480 )

• intestinal transit time is increased 2.3-fold compared to in wild-type mice

growth/size/body

decreased birth weight ( J:161480 )

decreased body mass index ( J:161480 )

increased lean body mass ( J:161480 )

decreased body size ( J:161480 )

postnatal growth retardation ( J:161480 )

adipose tissue

decreased total body fat amount ( J:161480 )

abnormal abdominal fat pad morphology ( J:161480 )

• mice exhibit reduced abdominal fat compared with wild-type mice

endocrine/exocrine glands

abnormal small intestine crypts of Lieberkuhn morphology ( J:161480 )

• small intestine crypts are disorganized, larger, and more abundant than in wild-type mice

abnormal pancreatic islet morphology ( J:161480 )

• mice exhibit more medium and large islets with centrally located alpha cells than in wild-type mice

small pancreatic islets ( J:161480 )

• mice exhibit a shift from large to single islets compared with wild-type mice

cellular

abnormal intestinal goblet cell morphology ( J:161480 )

• goblet cells in the large intestine are larger than in wild-type mice and mostly devoid of mucus

• however, goblet cell numbers are normal in the small intestine

abnormal small intestinal crypt cell proliferation ( J:161480 )

• at E19.5, crypt cell proliferation is increased compared to in wild-type mice

• in adult mice, crypt cell proliferation is increased with an up to 1.6-fold increase in cell turnover compared to in wild-type mice

Genotype
MGI:6711505

cn4

• Allelic Composition: Neurog3^tm3.1Ggr/Neurog3^tm3.1Ggr; Tg(CDX2-cre)101Erf/0
• Genetic Background: involves: 129S2/SvPas * C57BL/6J * SJL/J

digestive/alimentary system

digestive/alimentary phenotype ( J:285572 )

N • normal sensitivity to DSS-induced colitis

decreased enteroendocrine cell number ( J:285572 )

• EECs absent