Phenotypes associated with this allele

• Allele Symbol: Sema6b^tm1Hfu
• Allele Name: targeted mutation 1, Hajime Fujisawa
• Allele ID: MGI:4461232
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Sema6b^tm1Hfu/Sema6b^tm1Hfu	involves: 129S/SvEv * C57BL/6J	MGI:4461290
cx2	Sema6a^Gt(KST069)Byg/Sema6a^Gt(KST069)Byg Sema6b^tm1Hfu/Sema6b^tm1Hfu	involves: 129P2/OlaHsd * 129S/SvEv * C57BL/6J	MGI:4461294
cx3	Plxna2^tm1Hfu/Plxna2^tm1Hfu Sema6b^tm1Hfu/Sema6b^tm1Hfu	involves: 129S/SvEv * C57BL/6 * CBA	MGI:4461291
cx4	Plxna2^tm1Hfu/Plxna2^tm1Hfu Sema6b^tm1Hfu/Sema6b^+	involves: 129S/SvEv * C57BL/6 * CBA	MGI:4461293

Genotype
MGI:4461290

hm1

• Allelic Composition: Sema6b^tm1Hfu/Sema6b^tm1Hfu
• Genetic Background: involves: 129S/SvEv * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

abnormal hippocampal mossy fiber morphology ( J:160509 )

• suprapyramidal bundles are thicker while infrapyramidal bundles are longer than in wild-type mice

• mossy fiber spreading from the suprapyramidal and infrapyramidal bundles into the stratum radiatum and stratum oriens is inappropriate compared to in wild-type mice

• mossy fiber projections of P5 hippocampal slices cultured for 5 days exhibit very thin suprapyramidal bundles and spread widely within the stratum radiatum compared projections from wild-type cultures

• whether CA3 slices are cocultured with dentate gyrus slices from wild-type or Sema6b^tm1Hfu mice, mossy fibers spread abnormally into the stratum radiatum unlike similarly treated wild-type CA3 slices

• mossy fibers invade the inner molecular layer of the dentate gyrus unlike in wild-type mice

• mice exhibit abnormal projection of mossy cell axons compared to in wild-type mice

Genotype
MGI:4461294

cx2

• Allelic Composition: Sema6a^{Gt(KST069)Byg}/Sema6a^{Gt(KST069)Byg}; Sema6b^tm1Hfu/Sema6b^tm1Hfu
• Genetic Background: involves: 129P2/OlaHsd * 129S/SvEv * C57BL/6J

nervous system

abnormal hippocampal mossy fiber morphology ( J:160509 )

• the suprapyramidal bundle is thicker and the infrapyramidal bundle is longer than in Sema6b^tm1Hfu homozygotes

• more mossy fibers aberrantly spread into the stratum radiatum and stratum oriens compared to in Sema6b^tm1Hfu homozygotes

• however, mossy fiber spread into the stratum lacunosum-moleculare is normal

• mossy fibers invade the inner molecular layer of the dentate gyrus unlike in wild-type mice

Genotype
MGI:4461291

cx3

• Allelic Composition: Plxna2^tm1Hfu/Plxna2^tm1Hfu; Sema6b^tm1Hfu/Sema6b^tm1Hfu
• Genetic Background: involves: 129S/SvEv * C57BL/6 * CBA

nervous system

abnormal hippocampal mossy fiber morphology ( J:160509 )

• mossy fibers initially grow into the proximal parts of the infrapyramidal region and stratum pyramidalis of CA3c and eventually invade proximal parts of the suprapyramidal region of CA3ab to form the thinner suprapyramidal bundle compared to in wild-type mice

• some mossy fibers directly invade the stratum radiatum and stratum oriens of the CA3c and sometimes grow into the stratum pyramidalis of CA1 unlike in wild-type mice

• the suprapyramidal bundle is thinner than in wild-type mice and Plxna2^tm1Hfu Sema6a^{Gt(KST069)Byg} double homozygotes

Genotype
MGI:4461293

cx4

• Allelic Composition: Plxna2^tm1Hfu/Plxna2^tm1Hfu; Sema6b^tm1Hfu/Sema6b⁺
• Genetic Background: involves: 129S/SvEv * C57BL/6 * CBA

nervous system

abnormal hippocampal mossy fiber morphology ( J:160509 )

• mossy fibers are restricted to proximal parts of the infrapyramidal region and stratum pyramidalis in most parts of CA3, except distal CA3ab, and form a very thin suprapyramidal bundle compared to in wild-type mice