Phenotypes associated with this allele

• Allele Symbol: Add3^tm1.2Llp
• Allele Name: targeted mutation 1.2, Luanne Peters
• Allele ID: MGI:4462017
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Add3^tm1.2Llp/Add3^tm1.2Llp	129S1.129P2(B6)-Add3^tm1.2Llp	MGI:4462419
hm2	Add3^tm1.2Llp/Add3^tm1.2Llp	involves: 129P2/OlaHsd * 129S4/SvJaeSor * C57BL/6J * FVB/N	MGI:4462369
cx3	Add2^tm1Llp/Add2^tm1Llp Add3^tm1.2Llp/Add3^tm1.2Llp	involves: 129P2/OlaHsd * 129S4/SvJaeSor * C57BL/6J * FVB/N	MGI:4462409

Genotype
MGI:4462419

hm1

• Allelic Composition: Add3^tm1.2Llp/Add3^tm1.2Llp
• Genetic Background: 129S1.129P2(B6)-Add3^tm1.2Llp

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cardiovascular system

cardiovascular system phenotype ( J:161920 )

♀N • pulse rate, systolic, diastolic and mean arterial pressure assessed in females is normal

Genotype
MGI:4462369

hm2

• Allelic Composition: Add3^tm1.2Llp/Add3^tm1.2Llp
• Genetic Background: involves: 129P2/OlaHsd * 129S4/SvJaeSor * C57BL/6J * FVB/N

normal phenotype

no abnormal phenotype detected ( J:161920 )

• hematological values, osmotic fragility, and ektacytometry are all normal, red blood cells are normal in size and shape, and no change is found in the relative amounts of key membrane proteins in red blood cells, including alpha and beta adducin

• although there is a decrease by approximately 70% of the amount of alpha adducin in platelets, the bleed time, whole blood aggregation time, mean platelet volume, platelet numbers, structure and shape are all normal

Genotype
MGI:4462409

cx3

• Allelic Composition: Add2^tm1Llp/Add2^tm1Llp; Add3^tm1.2Llp/Add3^tm1.2Llp
• Genetic Background: involves: 129P2/OlaHsd * 129S4/SvJaeSor * C57BL/6J * FVB/N

hematopoietic system

anemia ( J:161920 )

abnormal erythrocyte morphology ( J:161920 )

• additional disruption of the gamma adducin gene does not exacerbate the phenotype of the beta adducin knockout and the changes in hematological values, altered red blood cell shapes and size, reticulocytosis, decreased hematocrit, and increased red blood cell fragility are the same, although the alpha adducin levels in red blood cell membranes is reduced to an almost undetectable level and the level in brain and spleen is reduced to half normal levels

decreased hematocrit ( J:161920 )

decreased mean corpuscular volume ( J:161920 )

anisopoikilocytosis ( J:161920 )

spherocytosis ( J:161920 )

reticulocytosis ( J:161920 )

increased erythrocyte osmotic fragility ( J:161920 )

• the increase in red blood cell fragility as assessed by osmotic lysis and ektacytometry is the same as in beta adducin knockouts