Phenotypes associated with this allele

• Allele Symbol: Setdb1^tm2.2Enl
• Allele Name: targeted mutation 2.2, En Li
• Allele ID: MGI:4462267
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Setdb1^tm2.2Enl/Setdb1^tm2.2Enl	involves: 129/Sv * C57BL/6	MGI:4462279
cn2	Setdb1^tm2.1Enl/Setdb1^tm2.2Enl	involves: 129/Sv * C57BL/6	MGI:4462280

Genotype
MGI:4462279

hm1

• Allelic Composition: Setdb1^tm2.2Enl/Setdb1^tm2.2Enl
• Genetic Background: involves: 129/Sv * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality between implantation and placentation, complete penetrance ( J:161089 )

• no mice are present at E8.5

Genotype
MGI:4462280

cn2

• Allelic Composition: Setdb1^tm2.1Enl/Setdb1^tm2.2Enl
• Genetic Background: involves: 129/Sv * C57BL/6

cellular

abnormal cell morphology ( J:161089 )

• embryonic stem cells transfected with a cre-ERT2 plasmid and treated with tamoxifen unlike untreated cells flatten and appear differentiated unlike untreated cells

• in embryonic stem cells transfected with a cre-ERT2 plasmid and treated with tamoxifen, expression of pluripotent markers is lost while expression of trophectoderm lineage markers is reduced compared with untreated cells

• in embryonic stem cells transfected with a cre-ERT2 plasmid and treated with tamoxifen, repressive histone H3K9 methylation is lost unlike in untreated cells

decreased cell proliferation ( J:161089 )

• in embryonic stem cells transfected with a cre-ERT2 plasmid and treated with tamoxifen unlike untreated cells