Phenotypes associated with this allele

• Allele Symbol: Coro7^{tm1a(EUCOMM)Hmgu}
• Allele Name: targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
• Allele ID: MGI:4462636
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Coro7^tm1a(EUCOMM)Hmgu/Coro7^tm1a(EUCOMM)Hmgu	C57BL/6N-Coro7^tm1a(EUCOMM)Hmgu	MGI:5811565

Genotype
MGI:5811565

hm1

• Allelic Composition: Coro7^{tm1a(EUCOMM)Hmgu}/Coro7^{tm1a(EUCOMM)Hmgu}
• Genetic Background: C57BL/6N-Coro7^{tm1a(EUCOMM)Hmgu}
• Cell Lines: HEPD0658_3_B04

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cellular

abnormal Golgi apparatus morphology ( J:235774 )

• primary dermal fibroblasts isolated from mutant neonates exhibit severe dispersal of the Golgi ribbon from the perinuclear position

• 38% of mutant fibroblasts show fragmented Golgi relative to 12% wild-type cells

• only 34% of mutant fibroblasts have the Golgi ribbon compact and lying within tens of micrometres from the nucleus relative to ~62% in wild-type cells

• scratch-wounded mutant fibroblasts in the first row reorganize their Golgi apparatus and reposition the centrosome (microtubule organizing center, MTOC) towards the leading edge faster than wild-type fibroblasts

• at 3 h post-wounding, the % of reoriented (polarized) Golgi and MTOC is significantly increased to 81% and 75% relative to 66% and 58%, respectively, in wild-type cells; however, no differences are noted at 7 h after wounding

• mutant fibroblasts show prominent actin filaments traversing the scattered Golgi stacks, suggesting that F-actin is increased at the Golgi apparatus

abnormal actin cytoskeleton morphology ( J:235774 )

• in a scratch-wound assay, 65% of mutant fibroblasts have reoriented their actin cytoskeleton perpendicularly to the wound edge at 3 h post-wounding relative to only 34% in wild-type cells where most actin fibers remain oriented parallel to the wound

• mutant fibroblasts exhibit massive F-actin filaments and a 1.5-fold increase in F-actin content relative to wild-type cells

abnormal cell adhesion ( J:235774 )

• mutant fibroblasts adhere more than wild-type cells and attach firmly to a fibronectin (FN)-coated surface within 30 min of seeding

• cell spreading and focal adhesions are significantly increased within 1 h of plating relative to wild-type cells

increased fibroblast cell migration ( J:235774 )

• in a scratch-wound assay, mutant fibroblasts exhibit a significantly higher mean migration speed than wild-type cells (15 um/h versus 8 um/h), leading to a faster gap closure after 20 h of wounding

homeostasis/metabolism

enhanced wound healing ( J:235774 )

• in a scratch-wound assay, mutant fibroblasts exhibit a significantly higher migration speed than wild-type cells, leading to a faster gap closure after 20 h of wounding

• however, the % of proliferating cells is normal, as shown by Ki-67 staining