Phenotypes associated with this allele

• Allele Symbol: Igh^tm2.1Fwa
• Allele Name: targeted mutation 2.1, Frederick W Alt
• Allele ID: MGI:4818343
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Igh^tm2.1Fwa/Igh^tm2.1Fwa	involves: 129S/SvEv * C57BL/6	MGI:4818345

Genotype
MGI:4818345

hm1

• Allelic Composition: Igh^tm2.1Fwa/Igh^tm2.1Fwa
• Genetic Background: involves: 129S/SvEv * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

immune system phenotype ( J:161437 )

N • anti-CD40/IL4- and TNP-ovalbumin-treated mice exhibit normal IgM levels

abnormal class switch recombination ( J:161437 )

• LPS-stimulated B cells fail to exhibit class switching to IgG1 unlike similarly treated wild-type cells

• B cells exhibit an increase in intraswitch recombination rates compared with wild-type cells

• B cells exhibit an increase in class switch recombination in the nonproductive alleles compared with wild-type cells

• however, switching to IgG3 and IgG2b is normal

abnormal somatic hypermutation frequency ( J:161437 )

• B cells exhibit a 10-fold increase in somatic hypermutation compared with wild-type cells

decreased mature B cell number ( J:161437 )

• mice stimulated with anti-CD40/IL4 exhibit a 160-fold decrease in the levels of IgG1+ B cells compared with similarly treated wild-type mice

increased IgE level ( J:161437 )

• 2.5-fold in anti-CD40/IL4 stimulated mice

• 3-fold in TNP-ovalbumin stimulated mice

• TNP-ovalbumin-treated mice exhibit a 20-fold increase in TNP-ovalbumin-specific IgE antibodies compared with similarly treated wild-type mice

decreased IgG1 level ( J:161437 )

• stimulated B cells do not secrete any detectable IgG1 unlike similarly treated wild-type cells

• TNP-ovalbumin-stimulated mice produce 50-fold less TNP-ovalbumin-specific IgG1 antibodies than similarly treated wild-type mice

hematopoietic system

abnormal class switch recombination ( J:161437 )

• LPS-stimulated B cells fail to exhibit class switching to IgG1 unlike similarly treated wild-type cells

• B cells exhibit an increase in intraswitch recombination rates compared with wild-type cells

• B cells exhibit an increase in class switch recombination in the nonproductive alleles compared with wild-type cells

• however, switching to IgG3 and IgG2b is normal

abnormal somatic hypermutation frequency ( J:161437 )

• B cells exhibit a 10-fold increase in somatic hypermutation compared with wild-type cells

decreased mature B cell number ( J:161437 )

• mice stimulated with anti-CD40/IL4 exhibit a 160-fold decrease in the levels of IgG1+ B cells compared with similarly treated wild-type mice

increased IgE level ( J:161437 )

• 2.5-fold in anti-CD40/IL4 stimulated mice

• 3-fold in TNP-ovalbumin stimulated mice

• TNP-ovalbumin-treated mice exhibit a 20-fold increase in TNP-ovalbumin-specific IgE antibodies compared with similarly treated wild-type mice

decreased IgG1 level ( J:161437 )

• stimulated B cells do not secrete any detectable IgG1 unlike similarly treated wild-type cells

• TNP-ovalbumin-stimulated mice produce 50-fold less TNP-ovalbumin-specific IgG1 antibodies than similarly treated wild-type mice