Phenotypes associated with this allele

• Allele Symbol: Tg(ACTA1-rtTA,tetO-cre)102Monk
• Allele Name: transgene insertion 102, Douglas Monks
• Allele ID: MGI:4818775
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Gt(ROSA)26Sor^tm1(DUX4)Sqh/Gt(ROSA)26Sor^+ Tg(ACTA1-rtTA,tetO-cre)102Monk/0	involves: 129S6/SvEvTac * C3H * C57BL/6	MGI:6280331
cn2	Bcl6^tm1Gdba/Bcl6^tm1Gdba Tg(ACTA1-rtTA,tetO-cre)102Monk/0	involves: C3H * C57BL/6	MGI:6294154
cn3	Etfdh^tm1c(EUCOMM)Hmgu/Etfdh^tm1c(EUCOMM)Hmgu Tg(ACTA1-rtTA,tetO-cre)102Monk/0	involves: C3H * C57BL/6 * C57BL/6N	MGI:7614304

Genotype
MGI:6280331

cn1

• Allelic Composition: Gt(ROSA)26Sor^tm1(DUX4)Sqh/Gt(ROSA)26Sor⁺; Tg(ACTA1-rtTA,tetO-cre)102Monk/0
• Genetic Background: involves: 129S6/SvEvTac * C3H * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

decreased survivor rate ( J:268959 )

• less than 3% of the expected 25% of mutant pups are born and those pups then die immediately

Genotype
MGI:6294154

cn2

• Allelic Composition: Bcl6^tm1Gdba/Bcl6^tm1Gdba; Tg(ACTA1-rtTA,tetO-cre)102Monk/0
• Genetic Background: involves: C3H * C57BL/6

adipose tissue

increased white adipose tissue amount ( J:271078 )

• dox-treated mice develop increased white adipose tissue in all depots, including the inguinal, interscapular, perigonadal, perinephric, and interscarpular adipose tissues but not brown adipose tissue

increased body fat mass ( J:271078 )

• mice treated with doxycycline (dox) for 7 days at 8 weeks of age exhibit a higher fat mass within 4 weeks after dox treatment

increased white fat cell size ( J:271078 )

• adipocyte size is increased in all white adipose tissue depots of dox-treated mice

growth/size/body

increased body fat mass ( J:271078 )

• mice treated with doxycycline (dox) for 7 days at 8 weeks of age exhibit a higher fat mass within 4 weeks after dox treatment

Genotype
MGI:7614304

cn3

• Allelic Composition: Etfdh^{tm1c(EUCOMM)Hmgu}/Etfdh^{tm1c(EUCOMM)Hmgu}; Tg(ACTA1-rtTA,tetO-cre)102Monk/0
• Genetic Background: involves: C3H * C57BL/6 * C57BL/6N

behavior/neurological

impaired coordination ( J:345923 )

• 6-month-old doxycycline-treated mice show decreased performance on the rotarod test and the two-limb hanging test in response to fasting, but not in the fed state

cellular

abnormal skeletal muscle fiber mitochondrial morphology ( J:345923 )

• some skeletal muscle mitochondria from doxycycline-treated mice appear swollen, empty and with cristae disorganization

abnormal oxidative phosphorylation ( J:345923 )

• isolated mitochondria from skeletal muscle shows reduced oxidative phosphorylation complex III (CIII) activity

• cytochrome c production in isolated mitochondria is reduced to 54% of wild-type

growth/size/body

decreased body weight ( J:345923 )

• doxycycline-treated mice exhibit reduced body weight by 5-6 months of age

weight loss ( J:345923 )

• 5-month-old doxycycline-treated mice show a poorer response to fasting, showing greater weight loss after fasting than controls

muscle

abnormal skeletal muscle fiber mitochondrial morphology ( J:345923 )

• some skeletal muscle mitochondria from doxycycline-treated mice appear swollen, empty and with cristae disorganization

abnormal muscle physiology ( J:345923 )

• mice show increased skeletal muscle de novo lipid synthesis in close contact with dysfunctional mitochondria

myopathy ( J:345923 )

• skeletal muscle from doxycycline-treated mice shows fiber shrinkage, sarcomere disorganization and debris, indicating myopathy