Phenotypes associated with this allele

• Allele Symbol: Npy^{tm1(rtTA,tetO-cre/EGFP)Snby}
• Allele Name: targeted mutation 1, Amanda Sainsbury
• Allele ID: MGI:4818970
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Npy^tm1(rtTA,tetO-cre/EGFP)Snby/Npy^+ Npy2r^tm1Hhz/Npy2r^tm1Hhz	involves: 129X1/SvJ * C57BL/6	MGI:5297095

Genotype
MGI:5297095

cn1

• Allelic Composition: Npy^{tm1(rtTA,tetO-cre/EGFP)Snby}/Npy⁺; Npy2r^tm1Hhz/Npy2r^tm1Hhz
• Genetic Background: involves: 129X1/SvJ * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

growth/size/body

growth/size/body region phenotype ( J:162000 )

N • weight gain monitored over 8-week period and body length of mutants after cre induction by intracerebroventricular injection of doxycycline is not different from treated controls or wild-type mice

increased body fat mass ( J:162000 )

• by whole body densitometry (DEXA), whole body fat mass in doxycycline-treated females is increased compared to wild-type controls; however, expressed as percent body weight, whole fat mass in doxycycline-treated females is not different from saline-treated mutant females

increased susceptibility to weight gain ( J:162000 )

• analyses over a 2-day period by indirect calorimetry show that male mutants (after cre induction) display a significant increase in body weight compared to treated controls; this transient, significant positive weight gain is observed in females also

adipose tissue

increased body fat mass ( J:162000 )

• by whole body densitometry (DEXA), whole body fat mass in doxycycline-treated females is increased compared to wild-type controls; however, expressed as percent body weight, whole fat mass in doxycycline-treated females is not different from saline-treated mutant females

decreased white adipose tissue mass ( J:162000 )

• doxycycline-treated females show a trend to reduced total fat (white adipose tissue) mass (as % body weight), but also display a trend to increased percent adiposity (total WAT weight compared to wild-type controls) suggesting an increase in relative fat mass

• this is not observed in males

increased white adipose tissue mass ( J:162000 )

• total mass of dissected white adipose tissue depots is increased (as % body weight) in doxycycline-treated females compared to saline-treated females

• this is not observed in males

behavior/neurological

behavior/neurological phenotype ( J:162000 )

N • no alterations in food intake in 15-week old mutants of either sex is observed with cre induction by intracerebroventricular injection of doxycycline; spontaneous food intake and intake after 24-hour fasting is similar to dox- or saline-treated controls and wild-type

N • over a 2-day period as measured by indirect calorimetry after cre induction, male mutants show trend toward increased food intake relative to controls, but females do not

abnormal locomotor activation ( J:162000 )

• after cre induction, females exhibit significantly decreased physical activity over a 2-day period relative to controls

• treated males do not show any alteration in activity

skeleton

increased trabecular bone volume ( J:162000 )

• doxycycline-treated mice show (slight (1.2-fold) but) significant increase in trabecular bone volume as well as trabecular number as determined by micro-CT scan analyses, without change in trabecular thickness

• no differences in cortical bone thickness or volume in distal femur is observed in dox-treated mice compared with controls

homeostasis/metabolism

decreased body temperature ( J:162000 )

• after cre induction, male mutants show trend toward decreased rectal temperature (and increased food intake) relative to controls

• females do not, although the transient weight gain is observed

increased oxygen consumption ( J:162000 )

• analyses over a 2-day period by indirect calorimetry show that female mutants after cre induction) display a significant increase in oxygen consumption notably during light phase with no change in the dark phase

• male mice do not show this or change in respiratory exchange ratio (RER)

abnormal glucose homeostasis ( J:162000 )

• doxycycline-treated females show improved fasting glycemia compared to controls

• doxycycline-treated males do not show this phenotype

increased liver triglyceride level ( J:162000 )

• doxycycline-treated females show an increase in hepatic triglyceride content compared to saline-treated females; in males, triglyceride content is significantly decreased

liver/biliary system

increased liver triglyceride level ( J:162000 )

• doxycycline-treated females show an increase in hepatic triglyceride content compared to saline-treated females; in males, triglyceride content is significantly decreased

renal/urinary system

renal/urinary system phenotype ( J:162000 )

N • daily water intake in mutants after with cre induction by intracerebroventricular injection of doxycycline is not different from control