Phenotypes associated with this allele

• Allele Symbol: Tg(Lck-cre)#Nik
• Allele Name: transgene insertion, Nigel Killeen
• Allele ID: MGI:4819511
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Tbc1d10c^tm1.1Psou/Tbc1d10c^tm1.2Psou Tg(Lck-cre)#Nik/0	involves: 129S2/SvPas * C57BL/6 * C57BL/6J * DBA/2	MGI:5578119
cn2	Rr7^tm3.1Kio/Rr7^+ Tg(Lck-cre)#Nik/0	involves: 129S4/SvJae * C57BL/6 * DBA/2	MGI:5305076
cn3	Myh9^tm5Rsad/Myh9^tm5Rsad Tg(Lck-cre)#Nik/0	involves: 129S6/SvEvTac * C57BL/6 * C57BL/6J * DBA/2	MGI:4838531

Genotype
MGI:5578119

cn1

• Allelic Composition: Tbc1d10c^tm1.1Psou/Tbc1d10c^tm1.2Psou; Tg(Lck-cre)#Nik/0
• Genetic Background: involves: 129S2/SvPas * C57BL/6 * C57BL/6J * DBA/2

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

immune system phenotype ( J:211913 )

N • mice exhibit normal early antigen-specific B cell response

Genotype
MGI:5305076

cn2

• Allelic Composition: Rr7^tm3.1Kio/Rr7⁺; Tg(Lck-cre)#Nik/0
• Genetic Background: involves: 129S4/SvJae * C57BL/6 * DBA/2

hematopoietic system

abnormal CD4-positive, alpha beta T cell morphology ( J:179326 )

• unlike wild-type cells, a portion of peripheral CD4+ T cells expressing CD8

• however, the number of CD4+(CD8+) decreases with age compared with Rr7^tm3.1Kio heterozygotes due to cre-mediated removal of the CD2 LCR

immune system

abnormal CD4-positive, alpha beta T cell morphology ( J:179326 )

• unlike wild-type cells, a portion of peripheral CD4+ T cells expressing CD8

• however, the number of CD4+(CD8+) decreases with age compared with Rr7^tm3.1Kio heterozygotes due to cre-mediated removal of the CD2 LCR

Genotype
MGI:4838531

cn3

• Allelic Composition: Myh9^tm5Rsad/Myh9^tm5Rsad; Tg(Lck-cre)#Nik/0
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6 * C57BL/6J * DBA/2

immune system

abnormal leukocyte migration ( J:164684 )

• the frequency of naive T cells in contact with high-endothelial venules is increased compared to in wild-type mice

• in adoptive transfer experiments, T cells exhibit shorter tracks, less displacement from their origin, reduced median speed, lower frequency of cells reaching peak instantaneous velocities, increased median turning angle, decreased average motility coefficient, and greater accumulation in lymph nodes due to impaired lymph node exit compared with wild-type cells

• naive T cells exhibit reduced Transwell chemotaxis in response to CCL21 compared with wild-type cells

• naive T cells exhibit inefficient crawling within a confined environment (8-4 um) compared with wild-type cells

• however, T cell crawling in a large width (12-20 um) lane is normal

abnormal leukocyte adhesion ( J:164684 )

• T cells exhibit a 2-fold greater adhesion to ICAM-1 under flow compared with similarly treated wild-type cells

• naive T cells exhibit inefficient crawling within a confined environment (8-4 um) compared with wild-type cells

• however, T cell crawling in a large width (12-20 um) lane is normal

decreased CD8-positive, alpha-beta T cell number ( J:164684 )

• in the periphery (lymph nodes and blood)

• however, thymic populations are normal

abnormal T cell physiology ( J:164684 )

• the frequency of naive T cells in contact with high-endothelial venules is increased compared to in wild-type mice

• T cells exhibit a 2-fold greater adhesion to ICAM-1 under flow compared with similarly treated wild-type cells

• migration of T cells on Transwell membranes is reduced compared with wild-type cells

• in adoptive transfer experiments, T cells exhibit shorter tracks, less displacement from their origin, reduced median speed, lower frequency of cells reaching peak instantaneous velocities, increased median turning angle, decreased average motility coefficient, and greater accumulation in lymph nodes due to impaired lymph node exit compared with wild-type cells

• naive T cells exhibit reduced Transwell chemotaxis in response to CCL21 compared with wild-type cells

• naive T cells exhibit inefficient crawling within a confined environment compared with wild-type cells

abnormal CD8-positive, alpha-beta T cell physiology ( J:164684 )

• despite a reduction in number, the percentage of CD8+ T cells in the lymph nodes tends to increase compared to in wild-type mice

abnormal lymph node cell ratio ( J:164684 )

• despite a reduction in number, the percentage of CD8+ T cells in the lymph nodes tends to increase compared to in wild-type mice

hematopoietic system

abnormal leukocyte migration ( J:164684 )

• the frequency of naive T cells in contact with high-endothelial venules is increased compared to in wild-type mice

• in adoptive transfer experiments, T cells exhibit shorter tracks, less displacement from their origin, reduced median speed, lower frequency of cells reaching peak instantaneous velocities, increased median turning angle, decreased average motility coefficient, and greater accumulation in lymph nodes due to impaired lymph node exit compared with wild-type cells

• naive T cells exhibit reduced Transwell chemotaxis in response to CCL21 compared with wild-type cells

• naive T cells exhibit inefficient crawling within a confined environment (8-4 um) compared with wild-type cells

• however, T cell crawling in a large width (12-20 um) lane is normal

abnormal leukocyte adhesion ( J:164684 )

• T cells exhibit a 2-fold greater adhesion to ICAM-1 under flow compared with similarly treated wild-type cells

• naive T cells exhibit inefficient crawling within a confined environment (8-4 um) compared with wild-type cells

• however, T cell crawling in a large width (12-20 um) lane is normal

decreased CD8-positive, alpha-beta T cell number ( J:164684 )

• in the periphery (lymph nodes and blood)

• however, thymic populations are normal

abnormal T cell physiology ( J:164684 )

• the frequency of naive T cells in contact with high-endothelial venules is increased compared to in wild-type mice

• T cells exhibit a 2-fold greater adhesion to ICAM-1 under flow compared with similarly treated wild-type cells

• migration of T cells on Transwell membranes is reduced compared with wild-type cells

• in adoptive transfer experiments, T cells exhibit shorter tracks, less displacement from their origin, reduced median speed, lower frequency of cells reaching peak instantaneous velocities, increased median turning angle, decreased average motility coefficient, and greater accumulation in lymph nodes due to impaired lymph node exit compared with wild-type cells

• naive T cells exhibit reduced Transwell chemotaxis in response to CCL21 compared with wild-type cells

• naive T cells exhibit inefficient crawling within a confined environment compared with wild-type cells

abnormal CD8-positive, alpha-beta T cell physiology ( J:164684 )

• despite a reduction in number, the percentage of CD8+ T cells in the lymph nodes tends to increase compared to in wild-type mice

cellular

abnormal leukocyte migration ( J:164684 )

• the frequency of naive T cells in contact with high-endothelial venules is increased compared to in wild-type mice

• in adoptive transfer experiments, T cells exhibit shorter tracks, less displacement from their origin, reduced median speed, lower frequency of cells reaching peak instantaneous velocities, increased median turning angle, decreased average motility coefficient, and greater accumulation in lymph nodes due to impaired lymph node exit compared with wild-type cells

• naive T cells exhibit reduced Transwell chemotaxis in response to CCL21 compared with wild-type cells

• naive T cells exhibit inefficient crawling within a confined environment (8-4 um) compared with wild-type cells

• however, T cell crawling in a large width (12-20 um) lane is normal

abnormal leukocyte adhesion ( J:164684 )

• T cells exhibit a 2-fold greater adhesion to ICAM-1 under flow compared with similarly treated wild-type cells

• naive T cells exhibit inefficient crawling within a confined environment (8-4 um) compared with wild-type cells

• however, T cell crawling in a large width (12-20 um) lane is normal