Phenotypes associated with this allele

• Allele Symbol: Hnrnpr^{tm1a(EUCOMM)Wtsi}
• Allele Name: targeted mutation 1a, Wellcome Trust Sanger Institute
• Allele ID: MGI:4820294
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Hnrnpr^tm1a(EUCOMM)Wtsi/Hnrnpr^tm1a(EUCOMM)Wtsi	C57BL/6-Hnrnpr^tm1a(EUCOMM)Wtsi	MGI:7620045

Genotype
MGI:7620045

hm1

• Allelic Composition: Hnrnpr^{tm1a(EUCOMM)Wtsi}/Hnrnpr^{tm1a(EUCOMM)Wtsi}
• Genetic Background: C57BL/6-Hnrnpr^{tm1a(EUCOMM)Wtsi}
• Cell Lines: EPD0609_5_A12

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

mortality/aging ( J:317395 )

N • mice are obtained in expected Mendelian ratios and survive to adulthood without overt phenotypic defects

growth/size/body

growth/size/body region phenotype ( J:317395 )

N • mice exhibit normal body weight up to adulthood and remain healthy at least up to 16 months of age

nervous system

nervous system phenotype ( J:317395 )

N • in culture, primary embryonic spinal motoneurons isolated from E12.5 embryos develop normally without any axonal growth defects or changes in growth cone size relative to wild-type motoneurons; axonal translocation of RN7SK (RNA, 7SK, nuclear), the top RNA interactor of HNRNPR, is unaffected

cellular

abnormal double-strand DNA break repair ( J:317395 )

• at 10- and 30 min recovery time after exposure to gamma irradiation (9 Gy), cultured motoneurons show enhanced nuclear gamma-H2AX immunosignals relative to wild-type motoneurons, indicating delayed DNA damage repair; however, gamma-H2AX levels return to basal (pre-irradiation) levels by 24 h in both genotypes

• similarly, motoneurons show enhanced nuclear gamma-H2AX signals at 10 min after treatment with etoposide (10 uM for 14 h); again, near-basal gamma-H2AX levels are observed after 24 h of recovery

induced chromosome breakage ( J:317395 )

• in culture, motoneurons grown for 6 DIV show increased accumulation of double-strand breaks (DSBs) upon exposure to genotoxic agents

• following exposure to gamma irradiation (9 Gy) or treatment with etoposide (10 uM for 14 h), nuclei of cultured motoneurons exhibit enhanced DNA damage (DSBs) with a significant increase in comet mean tail moment relative to wild-type motoneurons

• however, no overt DNA damage is noted in nuclei of cultured motoneurons under non-irradiated conditions or after DMSO treatment

homeostasis/metabolism

abnormal double-strand DNA break repair ( J:317395 )

• at 10- and 30 min recovery time after exposure to gamma irradiation (9 Gy), cultured motoneurons show enhanced nuclear gamma-H2AX immunosignals relative to wild-type motoneurons, indicating delayed DNA damage repair; however, gamma-H2AX levels return to basal (pre-irradiation) levels by 24 h in both genotypes

• similarly, motoneurons show enhanced nuclear gamma-H2AX signals at 10 min after treatment with etoposide (10 uM for 14 h); again, near-basal gamma-H2AX levels are observed after 24 h of recovery