All Phenotypes H2-T18<b> MGI Mouse

Phenotypes associated with this allele

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

premature death ( J:142515 )

• there is a 35% mortality rate resulting from colitis compared to 8.3% mortality of Tcra^tm1Mom homozygote controls

digestive/alimentary system

colitis ( J:142515 )

• mice have a higher incidence of colitis and a more severe disease than control mice that are only homozygote for the Tcra^tm1Mom null allele

• 83% of mice have colitis compared to 14% of controls at 30 weeks of age

• the first signs of disease occur at 15 weeks of age compared to 20 weeks of age in controls

• higher incidence of pathological lesions occurs in the colon of these mice, including loss of goblet cells, elongation of crypts, and inflammatory infiltration of the colon walls

• there is a 35% mortality rate resulting from colitis compared to 8.3% of controls

immune system

colitis ( J:142515 )

• mice have a higher incidence of colitis and a more severe disease than control mice that are only homozygote for the Tcra^tm1Mom null allele

• 83% of mice have colitis compared to 14% of controls at 30 weeks of age

• the first signs of disease occur at 15 weeks of age compared to 20 weeks of age in controls

• higher incidence of pathological lesions occurs in the colon of these mice, including loss of goblet cells, elongation of crypts, and inflammatory infiltration of the colon walls

• there is a 35% mortality rate resulting from colitis compared to 8.3% of controls

abnormal intraepithelial T cell number ( J:142515 )

• mice have higher numbers of gamma-delta and TCRbeta-beta IEL in the colon both before and after the onset of colitis compared to Tcra^tm1Mom homozygote controls

enlarged mesenteric lymph nodes ( J:142515 )

• mesenteric lymph nodes are enlarged in these mice with about a 2-fold increase in cell number compared to Tcra^tm1Mom homozygote controls

• this increase in cellularity occurs regardless if mice have developed colitis or not

increased interleukin-4 secretion ( J:142515 )

• TCRbeta-beta T cells isolated from the mesenteric lymph nodes produce around 4-fold more IL-4 than controls

hematopoietic system

abnormal intraepithelial T cell number ( J:142515 )

• mice have higher numbers of gamma-delta and TCRbeta-beta IEL in the colon both before and after the onset of colitis compared to Tcra^tm1Mom homozygote controls

Allelic Composition	H2-T18^b/H2-T18^b H2-T3^tm1Luc/H2-T3^tm1Luc
Genetic Background	C57BL/6-H2-T3^tm1Luc

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
H2-T18^b mutation (12 available); any H2-T18 mutation (54 available)
H2-T3^tm1Luc mutation (1 available); any H2-T3 mutation (25 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal intraepithelial T cell morphology ( J:142515 )

• differences at higher doses of anti-CD3 are not significant

• enhanced proliferation of IELs can be suppressed to wild-type levels when cultured in the presence of tumor cells bearing TL antigen

• IEL activation by low amounts of plate bound anti-CD3 is enhanced compared to controls

abnormal CD8 positive, alpha-beta intraepithelial T cell morphology ( J:142515 )

• colonic alpha-beta IELs expressing CD8-alpha homodimers have almost a 2-fold higher homeostatic proliferation rate in vivo

• enriched small intestine alpha-beta IEL have enhanced proliferation compared to controls in response to low amounts of plate bound anti-CD3

• when epithelial cells are removed from IEL culture, there is no longer a difference in the proliferative responses of IEL to anti-CD3

abnormal CD8-positive, gamma-delta intraepithelial T cell morphology ( J:142515 )

• colonic gamma-delta IELs expressing CD8-alpha homodimers have almost a 3-fold higher homeostatic proliferation rate in vivo

increased interferon-gamma secretion ( J:142515 )

• intestinal intraepithelial lymphocytes have enhanced IFN-gamma secretion in response to anti-CD3 stimulation