Phenotypes associated with this allele

• Allele Symbol: Myh9^{tm2(GFP/MYH10)Rsad}
• Allele Name: targeted mutation 2, Robert S Adelstein
• Allele ID: MGI:4829689
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Myh9^tm2(GFP/MYH10)Rsad/Myh9^tm2(GFP/MYH10)Rsad	involves: 129S4/SvJae * C57BL/6	MGI:4829692

Genotype
MGI:4829692

hm1

• Allelic Composition: Myh9^{tm2(GFP/MYH10)Rsad}/Myh9^{tm2(GFP/MYH10)Rsad}
• Genetic Background: involves: 129S4/SvJae * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:163702 )

• die between E9.5 and E10.5

embryo

embryo phenotype ( J:163702 )

N • unlike null mice, knock in embryos undergo gastrulation

abnormal vitelline vasculature morphology ( J:163702 )

• fewer visible vessels at E9.5 and E10.5

embryonic growth retardation ( J:163702 )

• display growth retardation that becomes more pronounced with age

abnormal placenta morphology ( J:163702 )

• placentas are composed predominantly of clusters of cuboidal trophoblasts

abnormal spongiotrophoblast layer morphology ( J:163702 )

• the spongiotrophoblast layer is difficult to discern

abnormal placenta labyrinth morphology ( J:163702 )

• the labyrinth layer is difficult to discern

abnormal placental labyrinth vasculature morphology ( J:163702 )

• embryonic blood vessels fail to invade the labyrinth layer

abnormal placenta size ( J:163702 )

• thinner and more compact compared to controls

pale yolk sac ( J:163702 )

• at E9.5 and E10.5

cardiovascular system

abnormal brain vasculature morphology ( J:163702 )

• the vascular network is less intricate at E10.5

abnormal placental labyrinth vasculature morphology ( J:163702 )

• embryonic blood vessels fail to invade the labyrinth layer

abnormal vascular branching morphogenesis ( J:163702 )

• the vascular networks of the head and trunk are less intricate at E10.5

abnormal vitelline vasculature morphology ( J:163702 )

• fewer visible vessels at E9.5 and E10.5

cellular

abnormal actin cytoskeleton morphology ( J:163702 )

• in MEFs actin filaments are thin, disorganized and often fail to align with the direction of migration

abnormal cell adhesion ( J:163702 )

• fewer and smaller focal adhesions are detected in MEFs and the stress fibers in the focal adhesions are in disarray

abnormal cell migration ( J:163702 )

• in a wound healing assay MEFs close the wound more rapidly compared to wild-type controls

• a transwell assay indicates that directional cell migration is impaired in MEFs

growth/size/body

embryonic growth retardation ( J:163702 )

• display growth retardation that becomes more pronounced with age

nervous system

abnormal brain vasculature morphology ( J:163702 )

• the vascular network is less intricate at E10.5