mortality/aging
• die between E9.5 and E10.5
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embryo
N |
• unlike null mice, knock in embryos undergo gastrulation
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• fewer visible vessels at E9.5 and E10.5
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• placentas are composed predominantly of clusters of cuboidal trophoblasts
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• the spongiotrophoblast layer is difficult to discern
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• the labyrinth layer is difficult to discern
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• embryonic blood vessels fail to invade the labyrinth layer
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• thinner and more compact compared to controls
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• at E9.5 and E10.5
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cellular
• in MEFs actin filaments are thin, disorganized and often fail to align with the direction of migration
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• fewer and smaller focal adhesions are detected in MEFs and the stress fibers in the focal adhesions are in disarray
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• a transwell assay indicates that directional cell migration is impaired in MEFs
• however, the speed at which MEFs close a wound is similar to that of wild-type cells
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cardiovascular system
• embryonic blood vessels fail to invade the labyrinth layer
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• fewer visible vessels at E9.5 and E10.5
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