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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Trim33tm1.2Los
targeted mutation 1.2, Regine Losson
MGI:4830323
Summary 6 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Trim33tm1.2Los/Trim33tm1.2Los involves: 129S2/SvPas * C57BL/6 * SJL MGI:4830327
cn2
Tg(CAG-cre)13Miya/0
Trim33tm1.1Los/Trim33tm1.2Los
involves: 129 * 129S2/SvPas * C57BL/6 * SJL MGI:4830330
cn3
Trim33tm1.1Los/Trim33tm1.2Los
Edil3Tg(Sox2-cre)1Amc/Edil3+
involves: 129S2/SvPas * C57BL/6 * CBA * SJL MGI:4830326
cn4
Smad4tm1Rdp/Smad4tm1.1Rdp
Trim33tm1.1Los/Trim33tm1.2Los
Tg(CAG-cre)13Miya/0
involves: 129S/SvEv * 129S2/SvPas * C57BL/6 * SJL MGI:4830328
cx5
Nodaltm1Rob/Nodaltm2Rob
Trim33tm1.2Los/Trim33tm1.2Los
involves: 129S/SvEv * 129S2/SvPas * C57BL/6 MGI:4830331
cx6
Nodaltm1Rob/Nodal+
Trim33tm1.2Los/Trim33tm1.2Los
involves: 129S/SvEv * 129S2/SvPas * C57BL/6 MGI:4830333


Genotype
MGI:4830327
hm1
Allelic
Composition
Trim33tm1.2Los/Trim33tm1.2Los
Genetic
Background
involves: 129S2/SvPas * C57BL/6 * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Trim33tm1.2Los mutation (0 available); any Trim33 mutation (71 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging

embryo
• defects in embryonic polarity and tissue patterning at E6.5
• defect in mesoderm formation
• lack a clear distinction between the epiblast and extraembryonic ectoderm
• expression analysis indicates absence of extraembryonic ectoderm at E5.5
• at E6.5 the proamniotic cavity is undivided
• abnormally broad at E5.5
• at E6.5 markers of the AVE are expanded around the epiblast

growth/size/body




Genotype
MGI:4830330
cn2
Allelic
Composition
Tg(CAG-cre)13Miya/0
Trim33tm1.1Los/Trim33tm1.2Los
Genetic
Background
involves: 129 * 129S2/SvPas * C57BL/6 * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(CAG-cre)13Miya mutation (1 available)
Trim33tm1.1Los mutation (0 available); any Trim33 mutation (71 available)
Trim33tm1.2Los mutation (0 available); any Trim33 mutation (71 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
embryo
• stated to be indistinguishable from mice homozygous for Trim33tm1.2Ros




Genotype
MGI:4830326
cn3
Allelic
Composition
Trim33tm1.1Los/Trim33tm1.2Los
Edil3Tg(Sox2-cre)1Amc/Edil3+
Genetic
Background
involves: 129S2/SvPas * C57BL/6 * CBA * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Edil3Tg(Sox2-cre)1Amc mutation (5 available); any Edil3 mutation (43 available)
Trim33tm1.1Los mutation (0 available); any Trim33 mutation (71 available)
Trim33tm1.2Los mutation (0 available); any Trim33 mutation (71 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• few embryos survive to E10

embryo
N
• unlike in homozygous germline null mice, no defects are seen in the anterior visceral endoderm or the extraembryonic ectoderm
• expression analysis indicates an almost radial expansion of the definitive endoderm at E7.5
• duplications of anterior axial mesendoderm tissues
• seen in about 1/3 of embryos that also have a defect in primitive streak morphology
• at E10 the few surviving embryos display open neural folds
• expanded in surviving embryos at E8.0
• duplications of node tissues
• about 1/3 of embryos lack an overtly elongated streak

nervous system
• at E10 the few surviving embryos display open neural folds
• at E10 the few surviving embryos display defective brain development

growth/size/body
• seen in about 1/3 of embryos that also have a defect in primitive streak morphology




Genotype
MGI:4830328
cn4
Allelic
Composition
Smad4tm1Rdp/Smad4tm1.1Rdp
Trim33tm1.1Los/Trim33tm1.2Los
Tg(CAG-cre)13Miya/0
Genetic
Background
involves: 129S/SvEv * 129S2/SvPas * C57BL/6 * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Smad4tm1.1Rdp mutation (0 available); any Smad4 mutation (48 available)
Smad4tm1Rdp mutation (0 available); any Smad4 mutation (48 available)
Tg(CAG-cre)13Miya mutation (1 available)
Trim33tm1.1Los mutation (0 available); any Trim33 mutation (71 available)
Trim33tm1.2Los mutation (0 available); any Trim33 mutation (71 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
embryo
• identical to mice null for Smad4 alone
• not induced at E5.5




Genotype
MGI:4830331
cx5
Allelic
Composition
Nodaltm1Rob/Nodaltm2Rob
Trim33tm1.2Los/Trim33tm1.2Los
Genetic
Background
involves: 129S/SvEv * 129S2/SvPas * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Nodaltm1Rob mutation (2 available); any Nodal mutation (42 available)
Nodaltm2Rob mutation (0 available); any Nodal mutation (42 available)
Trim33tm1.2Los mutation (0 available); any Trim33 mutation (71 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
embryo
N
• at E6.25 decrease in Nodal expression rescues patterning defects in the anterior visceral endoderm, extraembryonic ectoderm and mesoderm and the decrease in embryo size that are seen in Trim33 single homozygotes




Genotype
MGI:4830333
cx6
Allelic
Composition
Nodaltm1Rob/Nodal+
Trim33tm1.2Los/Trim33tm1.2Los
Genetic
Background
involves: 129S/SvEv * 129S2/SvPas * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Nodaltm1Rob mutation (2 available); any Nodal mutation (42 available)
Trim33tm1.2Los mutation (0 available); any Trim33 mutation (71 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
embryo
N
• the decrease in Nodal expression rescues patterning defects in the extraembryonic ectoderm and mesoderm and the decrease in embryo size that are seen in Trim33 single homozygotes





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last database update
12/10/2024
MGI 6.24
The Jackson Laboratory