Phenotypes associated with this allele

• Allele Symbol: Macf1^tm2.1Liem
• Allele Name: targeted mutation 2.1, Ronald K H Liem
• Allele ID: MGI:4831018
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Macf1^tm2.1Liem/Macf1^tm2.2Liem Tg(Nes-cre)1Kln/0	involves: 129S6/SvEvTac * C57BL/6 * FVB/N * SJL	MGI:4831040

Genotype
MGI:4831040

cn1

• Allelic Composition: Macf1^tm2.1Liem/Macf1^tm2.2Liem; Tg(Nes-cre)1Kln/0
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6 * FVB/N * SJL

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

neonatal lethality, complete penetrance ( J:164146 )

• most die within 24 hours of birth due to respiratory insufficiency

• all mice die by 36 hours after birth

nervous system

nervous system phenotype ( J:164146 )

N • mice exhibit normal diaphragm innervation and endplate formation

abnormal neuronal migration ( J:164146 )

• cortical neuronal migration is delayed compared to in mice lacking the cre transgene

• cortical neurons exhibit abnormal positioning in traversing the cortical plate compared to in mice lacking the cre transgene

• however, mice exhibit normal radial glia and inside-out cortical neurons migration

abnormal brain morphology ( J:164146 )

• thalamocortical fibers are less compact and thinner with many axons extending tangentially towards the cortical surface unlike in mice lacking the cre transgene

abnormal lateral ventricle morphology ( J:164146 )

• small and misshaped

small lateral ventricles ( J:164146 )

abnormal hippocampal commissure morphology ( J:164146 )

abnormal anterior commissure morphology ( J:164146 )

abnormal cerebral hemisphere morphology ( J:164146 )

• mice exhibit an abnormal accumulation of cells in the intermediate zone of the dorsal and ventral cerebrum unlike in mice lacking the cre transgene

abnormal dentate gyrus morphology ( J:164146 )

• less compact

abnormal hippocampus pyramidal cell layer ( J:164146 )

• the pyramidal neuronal layer is decreased compared to in mice lacking the cre transgene

• an additional pyramidal layer is positioned outside of the primary pyramidal layer especially in the CA1-CA2 region unlike in mice lacking the cre transgene

• in the caudal regions, the pyramidal cell layer exhibits undulations not observed in mice lacking the cre transgene

ectopic hippocampus pyramidal cells ( J:164146 )

• an additional pyramidal layer is positioned outside of the primary pyramidal layer especially in the CA1-CA2 region unlike in mice lacking the cre transgene

abnormal cerebral cortex morphology ( J:164146 )

• mice exhibit a blurring of the compartmental boundaries in the cortex compared with mice lacking the cre transgene

abnormal stratification in cerebral cortex ( J:164146 )

• at E17.5, mice exhibit disorganization of stratified structures in the cortex with loss of the distinctive tri-laminar appearance compared with mice lacking the cre transgene

• cortical layers are partially mixed unlike in mice lacking the cre transgene

• however, preplate splitting is normal

thin cerebral cortex ( J:164146 )

• the marginal and ventricular zones are thinner than in mice lacking the cre transgene

respiratory system

respiratory distress ( J:164146 )

• most die within 24 hours of birth due to respiratory insufficiency

homeostasis/metabolism

cyanosis ( J:164146 )

cellular

abnormal neuronal migration ( J:164146 )

• cortical neuronal migration is delayed compared to in mice lacking the cre transgene

• cortical neurons exhibit abnormal positioning in traversing the cortical plate compared to in mice lacking the cre transgene

• however, mice exhibit normal radial glia and inside-out cortical neurons migration