mortality/aging
• homozygous embryos are present in expected numbers at E3.5
• no homozygous embryos at E10.5
• embryonic death around the time of implantation
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embryo
• E3.5 embryos cultured in vitro for 3 days contain extensive apoptotic cells
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• smaller than controls after E3.5
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• internal embryonic structure is disorganized subsequent to E3.5
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• inner cell mass is reduced after E3.5
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cellular
• mitotic cells are mostly in prometaphase (72%)
• chromosomes remain dispersed in cytoplasm
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• spindle structures are distorted
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• increased number of mitotic cells
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• E3.5 embryos cultured in vitro for 3 days contain extensive apoptotic cells
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growth/size/body
• smaller than controls after E3.5
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