Phenotypes associated with this allele

• Allele Symbol: Tg(Msx2-En1)86Alj
• Allele Name: transgene insertion 86, Alexandra L Joyner
• Allele ID: MGI:4834774
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
tg1	Tg(Msx2-En1)86Alj/Tg(Msx2-En1)86Alj	involves: Swiss Webster	MGI:4834818
tg2	Tg(Msx2-En1)86Alj/0	involves: Swiss Webster	MGI:4834817

Genotype
MGI:4834818

tg1

• Allelic Composition: Tg(Msx2-En1)86Alj/Tg(Msx2-En1)86Alj
• Genetic Background: involves: Swiss Webster

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

limbs/digits/tail

abnormal limb morphology ( J:62572 )

abnormal digit morphology ( J:62572 )

• in some animals (20%), only digits 4-5 are malformed

adactyly ( J:62572 )

• in many animals (70%), forelimbs have only 1-2 digits

abnormal forelimb morphology ( J:62572 )

• forelimbs are less severely affected than hindlimbs

• forelimbs often lack some digits (70%) except 1-2; 10% of animals show a truncation below the humerus

abnormal hindlimb morphology ( J:62572 )

• all mice display limb deformities, with hemizygotes displaying less severe defects

abnormal femur morphology ( J:62572 )

• only small fragments of femur are present in severe cases

absent fibula ( J:62572 )

• some animals (20%) have tibia and distal cartilaginous fragments

absent hindlimb ( J:62572 )

• most animals lack hindlimbs, only small fragments of femur are present (80%)

skeleton

abnormal femur morphology ( J:62572 )

• only small fragments of femur are present in severe cases

absent fibula ( J:62572 )

• some animals (20%) have tibia and distal cartilaginous fragments

Genotype
MGI:4834817

tg2

• Allelic Composition: Tg(Msx2-En1)86Alj/0
• Genetic Background: involves: Swiss Webster

limbs/digits/tail

abnormal limb morphology ( J:62572 )

• all mice display limb deformities

abnormal autopod morphology ( J:62572 )

adactyly ( J:62572 )

• most common forelimb phenotype is loss of digits 3, 4, and/or 5 (69% of mice); presence of only a single digit occurs rarely (3%)

ectopic digits ( J:62572 )

• some mice have hindpaw ectopic digits (8% of limbs) protruding from ventral handplate or distal zeugopod; these digits have poorly formed body elements

polydactyly ( J:62572 )

• extra partial digits are occasionally seen in forelimbs (10%) and hindlimbs (13%); ectopic digits do not show preferential anterior-posterior localization

abnormal forelimb morphology ( J:62572 )

• forelimbs are less severely affected than hindlimbs

bowed radius ( J:62572 )

• when partial ulna loss occurs, radius is bowed

abnormal ulna morphology ( J:62572 )

absent ulna ( J:62572 )

• complete loss of the ulna is observed in about 8% of mice

short ulna ( J:62572 )

• complete loss of the ulna is observed in about 8% of mice

abnormal hindlimb morphology ( J:62572 )

abnormal digit development ( J:62572 )

• hindlimbs often shown nearly complete absence of autopod with one malformed digit composed of cartilaginous fragments distal to the zeugopod

skeleton

bowed radius ( J:62572 )

• when partial ulna loss occurs, radius is bowed

abnormal ulna morphology ( J:62572 )

absent ulna ( J:62572 )

• complete loss of the ulna is observed in about 8% of mice

short ulna ( J:62572 )

• complete loss of the ulna is observed in about 8% of mice