mortality/aging
• although some mice are present E18.5, no mice are present at birth
|
• most mice die between E16.5 and E18.5
|
nervous system
N |
• mice exhibit normal neuronal viability and presynaptic release probability
• mice exhibit normal kainic acid-induced currents
• neurons exhibit normal synapse density per dendrite length, presynaptic maturation of synapses, and spatial matching of pre-and postsynapses
|
• cultured neurons are smaller than similarly treated wild-type neurons
• however, neuron survival in culture is normal
|
• dendrites are more immature (shorter with less branching) than on wild-type neurons
• however, initial neuritogenesis is normal
|
• the number of Synapsin+ presynaptic puncta is reduced compared to in wild-type neurons
• the number of Synapsin+ and Bassoon+ synpatic puncta is decreased compared to in wild-type neurons
|
• the size of the readily releasable vesicle pool is decreased compared to in wild-type neurons
|
• the amplitude of excitatory postsynaptic currents is reduced compared to in wild-type mice
|
• the frequency of miniature excitatory postsynaptic currents (mEPSCs) is reduced compared to in wild-type mice
• however, the amplitude of mEPSCs is normal
|
cardiovascular system
hemorrhage
(
J:164745
)
• 50% of mice exhibit subcutaneous bleeding
|
growth/size/body
• beginning at E11.5
|