Phenotypes associated with this allele

• Allele Symbol: Dgkb^{Tn(sb-lacZ,GFP)IR3.PV122Jtak}
• Allele Name: transposon insertion IR3.PV122, Junji Takeda
• Allele ID: MGI:4836984
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Dgkb^Tn(sb-lacZ,GFP)IR3.PV122Jtak/Dgkb^Tn(sb-lacZ,GFP)IR3.PV122Jtak	involves: C3H * C57BL/6 * C57BL/6N * DBA/2 * ICR	MGI:5616910
hm2	Dgkb^Tn(sb-lacZ,GFP)IR3.PV122Jtak/Dgkb^Tn(sb-lacZ,GFP)IR3.PV122Jtak	involves: C3H * C57BL/6 * DBA/2 * ICR	MGI:4837002

Genotype
MGI:5616910

hm1

• Allelic Composition: Dgkb^{Tn(sb-lacZ,GFP)IR3.PV122Jtak}/Dgkb^{Tn(sb-lacZ,GFP)IR3.PV122Jtak}
• Genetic Background: involves: C3H * C57BL/6 * C57BL/6N * DBA/2 * ICR

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

impaired behavioral response to xenobiotic ( J:204267 )

• methylphenidate (MPH) treatment increases the recognition index of mutants in the object-based attention test compared to wild-type mice which show decreased recognition index in response to MPH

• MPH treatment does not increase locomotor activity as in wild-type mice, except for a slight increase at 35 minutes following treatment, indicating that mutants are hyporesponsive to MPH-induced behavior change

• MPH treatment does not decrease stereotyped behaviors during short 5 minute testing as it does in wild-type mice

• MPH treatment, however, reduces the rearing behavior of mutants to levels seen in vehicle-treated wild-type mice

abnormal response to novel object ( J:204267 )

• in the object-based attention test in which mice are exposed to objects for 3 minutes during training, mutants spend less time on the novel object than wild-type mice during the retention session

• this difference is not seen when mice are exposed to the objects for 6 minutes before a retention session

• methylphenidate (MPH) treatment increases the recognition index of mutants in the object-based attention test compared to wild-type mice which show a decreased recognition index in response to MPH

increased vertical activity ( J:204267 )

• mice exhibit increased rearing behaviors in a novel environment

increased locomotor activity ( J:204267 )

• mice exhibit increased locomotor activity

Genotype
MGI:4837002

hm2

• Allelic Composition: Dgkb^{Tn(sb-lacZ,GFP)IR3.PV122Jtak}/Dgkb^{Tn(sb-lacZ,GFP)IR3.PV122Jtak}
• Genetic Background: involves: C3H * C57BL/6 * DBA/2 * ICR

behavior/neurological

abnormal long-term spatial reference memory ( J:163104 )

• in contrast to wild-type mice which spent increased time in the old target quadrant, mice spent almost equal time in the four areas of the Morris water maze test

• mice swam further away from where the platform used to be than wild-type mice

abnormal spatial working memory ( J:163104 )

• a significant decrease in alternation compared to their wild-type litter mates in a Y-maze

nervous system

abnormal hippocampus neuron morphology ( J:163104 )

• in culture the number of branches, spines as well as total length of neurites and branches, is significantly decreased compared to wild-type

abnormal synapse morphology ( J:163104 )

• The average number of synaptic junctions detected by electron microscopy is decreased compared to wild-type

reduced long-term potentiation ( J:163104 )

• a marked reduction of LTP was observed in the CA1 region

homeostasis/metabolism

abnormal lipid level ( J:163104 )

• phosphatidic acid content is reduced to about 70% wild-type levels in the hippocampus