Phenotypes associated with this allele

• Allele Symbol: Ptk2^tm1.1Itl
• Allele Name: targeted mutation 1.1, inGenious Targeting Laboratory
• Allele ID: MGI:4837147
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Ptk2^tm1.1Itl/Ptk2^tm1.1Itl	involves: 129S/SvEv * C57BL/6 * SJL	MGI:4837172

Genotype
MGI:4837172

hm1

• Allelic Composition: Ptk2^tm1.1Itl/Ptk2^tm1.1Itl
• Genetic Background: involves: 129S/SvEv * C57BL/6 * SJL

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Embryonic lethality and vascular defects in Ptk2^tm1.1Itl/Ptk2^tm1.1Itl embryos

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:165360 )

• mice die between E9.5 and E10.5

cardiovascular system

abnormal vascular development ( J:165360 )

• at E9.5, mice exhibit abnormal vascular patterning in the head, heart, and somite structures compared to in wild-type mice

abnormal vitelline vasculature morphology ( J:165360 )

• at E9.5, more than 50 mice lack blood filled vessels in the yolk sac unlike wild-type mice

abnormal heart morphology ( J:165360 )

• at E9.5

hemorrhage ( J:165360 )

• mice exhibit hemorrhage into the extended allantois unlike wild-type mice

absent heartbeat ( J:165360 )

• at E9.5

embryo

abnormal vitelline vasculature morphology ( J:165360 )

• at E9.5, more than 50 mice lack blood filled vessels in the yolk sac unlike wild-type mice

absent somites ( J:165360 )

• at E9.5

enlarged allantois ( J:165360 )

failure of chorioallantoic fusion ( J:165360 )

abnormal vitelline vascular remodeling ( J:165360 )

• capillary plexus formation and endothelial cell branching are defective compared to in wild-type mice

• however, no increase in apoptosis is observed

cellular

abnormal cell physiology ( J:165360 )

• mouse embryonic fibroblasts form exaggerated numbers of focal adhesions connected to elevated numbers of actin stress fibers and multiple lamellipodial projections without a defined front-back distribution unlike in wild-type cells

abnormal fibroblast migration ( J:165360 )

• mouse embryonic fibroblasts exhibit limited and random cell movement after addition of serum to culture media compared with similarly treated wild-type cells

growth/size/body

abnormal head morphology ( J:165360 )

• at E9.5