Phenotypes associated with this allele

• Allele Symbol: Rad50^tm2.1Flv
• Allele Name: targeted mutation 2.1, Richard A Flavell
• Allele ID: MGI:4837633
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Rad50^tm2.1Flv/Rad50^tm2.1Flv	B6.129S6-Rad50^tm2.1Flv	MGI:5514500
cn2	Rad50^tm2.1Flv/Rad50^tm2.2Flv Tg(Cd4-cre)1Cwi/0	involves: 129S6/SvEvTac * C57BL/6 * DBA/2	MGI:4838166

Genotype
MGI:5514500

hm1

• Allelic Composition: Rad50^tm2.1Flv/Rad50^tm2.1Flv
• Genetic Background: B6.129S6-Rad50^tm2.1Flv

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

decreased interleukin-13 secretion ( J:196154 )

• from CD4+ T cells cultured under in vitro Th2-polarizing conditions with anti-CD3 antibodies

• from splenocytes of mice stimulated with ovalbumin and restimulated in vitro with ovalbumin

• from skin draining lymph nodes of mice subjected to epicutaneous priming followed by restimulation with ovalbumin in the presence of splenic antibody presenting cells

decreased interleukin-4 secretion ( J:196154 )

• from CD4+ T cells cultured under in vitro Th2-polarizing conditions with anti-CD3 antibodies

decreased interleukin-5 secretion ( J:196154 )

• from CD4+ T cells cultured under in vitro Th2-polarizing conditions with anti-CD3 antibodies

• from splenocytes of mice stimulated with ovalbumin and restimulated in vitro with ovalbumin

Genotype
MGI:4838166

cn2

• Allelic Composition: Rad50^tm2.1Flv/Rad50^tm2.2Flv; Tg(Cd4-cre)1Cwi/0
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6 * DBA/2

immune system

immune system phenotype ( J:165055 )

N • CD4 T cell differentiation is normal

abnormal CD4-positive, alpha beta T cell morphology ( J:165055 )

• stimulated CD4+ T cells are larger than similarly treated wild-type cells

decreased IgE level ( J:165055 )

• in ovalbumin challenged mice

abnormal T-helper 1 physiology ( J:165055 )

• under Th1 conditions, splenocytes produce less IFN-gamma than similarly treated wild-type cells

abnormal T-helper 2 physiology ( J:165055 )

• under Th2 conditions, splenocytes produce less IL4, IL5, and IL13 compared with similarly treated wild-type cells

decreased interferon-gamma secretion ( J:165055 )

• in splenocytes under Th1 conditions

decreased interleukin-13 secretion ( J:165055 )

• in splenocytes under Th2 conditions

decreased interleukin-4 secretion ( J:165055 )

• in splenocytes under Th2 conditions

decreased interleukin-5 secretion ( J:165055 )

• in splenocytes under Th2 conditions

decreased inflammatory response ( J:165055 )

• ovalbumin-treated mice exhibit no increase in eosinophils and lymphocytes in the bronchoalveolar lavage fluid, reduced infiltration of cells in the peribronchiolar and perivascular regions of the lung and decreased mucus production unlike wild-type mice

respiratory system

abnormal airway resistance ( J:165055 )

• following methacholine treatment, ovalbumin sensitized and challenged mice fail to exhibit an increase in airway resistance unlike similarly treated wild-type mice

decreased airway responsiveness ( J:165055 )

• following methacholine treatment, ovalbumin sensitized and challenged mice fail to exhibit airway hyperresponsiveness unlike similarly treated wild-type mice

cellular

abnormal DNA methylation ( J:165055 )

• Th2 cells exhibit a dramatic increase in DNA methylation in the il4 promoter and CNS-1 region unlike similarly treated wild-type cells that exhibit a decrease in DNA methylation in this region

hematopoietic system

abnormal CD4-positive, alpha beta T cell morphology ( J:165055 )

• stimulated CD4+ T cells are larger than similarly treated wild-type cells

decreased IgE level ( J:165055 )

• in ovalbumin challenged mice

abnormal T-helper 1 physiology ( J:165055 )

• under Th1 conditions, splenocytes produce less IFN-gamma than similarly treated wild-type cells

abnormal T-helper 2 physiology ( J:165055 )

• under Th2 conditions, splenocytes produce less IL4, IL5, and IL13 compared with similarly treated wild-type cells