Analysis Tools|
Allele Symbol Allele Name Allele ID |
Adam10tm2Psa targeted mutation 2, Paul Saftig MGI:4838238 |
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| Summary |
4 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• mice do not show overt abnormalities, even though cre expression is detected in esophagus and the stomach; no differences in histology are detected in esophagus and glandular hindstomach relative to controls
• mice grow at similar rate to littermates, as shown by body weight measurements
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• forestomach hypoplasia is detected with decreased depth of gastric pits in adult mice
• no digestive problems are observed
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• only small number of mutant mice survived more than 12 h after birth
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• seen in dying embryos
• macroscopically mutant embryos did not display gross abnormalities
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• seen in dying embryos
• macroscopically mutant embryos did not display gross abnormalities
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• a premature differentiation of neural progenitor cells into postmitotic neuron
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• reduction of the ganglionic eminence and disrupted organization of the cortical region in E15.5 and later embryos
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• a premature differentiation of neural progenitor cells into postmitotic neuron
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• endometrial endothelial cells are unable to support a normal decidualization reaction resulting in pregnancy loss between 5.5 days post conception (dpc) and 6.5 dpc
• genes associated with embryonic and trophoblast lineage development and decidualization are downregulated, consistent with the observed pregnancy loss
• however, corpora lutea (CL) histology and the distribution pattern of endothelial cells in the CL are normal at 5.5 dpc
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• at 5.5 dpc, the decidualization reaction is less widely distributed throughout the stroma and less organized than in controls; however, the ability of endometrial stromal cells to differentiate into decidual cells is normal
• after induction of artificial decidualization, the weights of scratched uterine horns are significantly lower than those of controls, indicating that decidualization is impaired independently of embryonic signals
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• at 5.5 dpc, implantation sites are round instead of oval and have a smaller diameter than control sites; the area of the proximal decidual zone is significantly reduced and a less developed embryonic body is observed
• at 5.5 dpc, embryonic crypt formation is abnormal and large red blood cell-filled spaces are frequently seen close to the implantation sites, indicating aberrant blood vessel formation
• at 5.5 dpc, the area of the endometrial fraction is significantly reduced, and implantation sites show a disorganized vasculature with an increase in endothelial cell coverage
• however, no significant changes in tissue perfusion, hypoxia levels, cell proliferation in the endometrium, or distribution of immune cells are observed in the implantation site
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• at 5.5 dpc, the implanting embryo remains localized in the anti-mesometrial side of the lumen rather than having invaded the maternal decidua as in controls
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• at 5.5 dpc, luminal closure is incomplete
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• at 5.5 dpc, all analyzed females are pregnant but show a ~20% reduction in the number of implantation sites relative to control females
• most or all implantation sites are completely resorbed by 6.5 dpc
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• when mated with control Adam10tm2Psa homozygous males for a total of 4 months, 6 of 8 females fail to give birth
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• when mated with control males for a 4 month-period, 2 of 8 females produce a relatively small number of offspring (9 pups or 10 pups each, over the course of 3 litters); on average, females give birth to a total of 2.3 +/- 4.4 pups per mouse over 4 months versus 22.88 +/- 3.1 pups per control female
• when mated with wild-type males (129S1/SvImj), 2 of 5 females produce a total of 8 pups in 2 litters, with an average of 1.6 +/- 2.6 pups per female over 4 months
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• when females are mated with control males for a 4 month-period, average litter size 3.18 +/- 0.8 versus 9.96 +/- 2.2 in control x control matings
• when females are mated with wild-type males (129S1/SvImj), average litter size is only 2.7 +/- 2.1
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• at 5.5 dpc, the decidualization reaction is less widely distributed throughout the stroma and less organized than in controls; however, the ability of endometrial stromal cells to differentiate into decidual cells is normal
• after induction of artificial decidualization, the weights of scratched uterine horns are significantly lower than those of controls, indicating that decidualization is impaired independently of embryonic signals
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• females show a disorganized vasculature during the initiation of decidualization: at 5.5 dpc, vessels closer to the implantation sites are abnormally large, vein-like, and organized in honeycomb-like structures, unlike in controls
• an accumulation of CD31 + cells surrounding the luminal epithelium and increased vascularization of the area proximal to the implantation site are observed, unlike in the control area where vascular density is reduced
• increased endomucin (Emcn) and VEGFR2 staining of the enlarged abnormal vasculature surrounding the embryo in implantation sites is suggestive of a defect in endothelial ADAM10/Notch signaling
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• females show a significant increase in the area covered by CD31 + endothelial cells in the endometrium at 5.5 dpc, but not at earlier time points (3.5 or 4.5 dpc) during pregnancy
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/19/2024 MGI 6.24 |
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