Phenotypes associated with this allele

• Allele Symbol: Spesp1^tm1Osb
• Allele Name: targeted mutation 1, Research Institute for Microbial Diseases, Osaka University
• Allele ID: MGI:4838302
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Spesp1^tm1Osb/Spesp1^tm1Osb	involves: 129S2/SvPas * C57BL/6	MGI:4838303
ht2	Spesp1^tm1Osb/Spesp1^+	involves: 129S2/SvPas * C57BL/6	MGI:4838304

Genotype
MGI:4838303

hm1

• Allelic Composition: Spesp1^tm1Osb/Spesp1^tm1Osb
• Genetic Background: involves: 129S2/SvPas * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

reproductive system

abnormal acrosome morphology ( J:159611 )

♂ • in almost all of the sperm, the equatorial segment membrane was completely lost in mutant sperm among acrosome-reacted sperm

♂ • The acrosome-intact mutant sperm were morphologically similar to wild-type sperm

delayed fertilization ( J:159611 )

• sperm from homozygous null males showed a delayed onset of fertilization ratio compared with wild-type males

• mutant male sperm have defects in migrating into the oviduct

• no decrease in number of ejaculated sperm nor decreased sperm motility

decreased fertilization frequency ( J:159611 )

• Although homozygous null females show normal fertility, both heterozygous and homozygous null males had statistically significant fewer offspring than wild-type males both in vivo and in vitro

• There was no difference observed in the number, motility and swimming patterns of sperm obtained from mutant mice compared with those of wild-type mice.

abnormal sperm physiology ( J:159611 )

♂ • using zona-free eggs, sperm from both heterozygous and homozygous null mice had significantly less fusion ability compared with wild-type sperm in vitro

cellular

abnormal acrosome morphology ( J:159611 )

♂ • in almost all of the sperm, the equatorial segment membrane was completely lost in mutant sperm among acrosome-reacted sperm

♂ • The acrosome-intact mutant sperm were morphologically similar to wild-type sperm

Genotype
MGI:4838304

ht2

• Allelic Composition: Spesp1^tm1Osb/Spesp1⁺
• Genetic Background: involves: 129S2/SvPas * C57BL/6

reproductive system

decreased fertilization frequency ( J:159611 )

• Although homozygous null females show normal fertility, both heterozygous and homozygous null males had statistically significant fewer offspring than wild-type males both in vivo and in vitro

• There was no difference observed in the number, motility and swimming patterns of sperm obtained from mutant mice compared with those of wild-type mice.

abnormal sperm physiology ( J:159611 )

♂ • using zona-free eggs, sperm from both heterozygous and homozygous null mice had significantly less fusion ability compared with wild-type sperm in vitro