Phenotypes associated with this allele

• Allele Symbol: Ago2^tm1.1Tara
• Allele Name: targeted mutation 1.1, Alexander Tarakhovsky
• Allele ID: MGI:4839762
• Summary: 5 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Ago2^tm1.1Tara/Ago2^tm1.1Tara Tg(KRT14-cre)1Efu/0	involves: 129P2/OlaHsd	MGI:5319525
cn2	Ago1^tm1.1Tara/Ago1^tm1.1Tara Ago2^tm1.1Tara/Ago2^tm1.1Tara Tg(KRT14-cre)1Efu/0	involves: 129P2/OlaHsd	MGI:5319527
cn3	Ago2^tm1.1Tara/Ago2^tm1.1Tara Tg(Mx1-cre)1Cgn/0	involves: 129P2/OlaHsd * 129S4/SvJaeSor * C57BL/6 * CBA	MGI:4839763
cn4	Ago2^tm1.1Tara/Ago2^tm1.1Tara Lep^ob/Lep^ob Tg(Ins2-cre)23Herr/0	involves: 129P2/OlaHsd * C57BL/6J * CBA/J	MGI:5585185
cn5	Ago2^tm1.1Tara/Ago2^tm1.1Tara Tg(Ins2-cre)23Herr/0	involves: 129P2/OlaHsd * C57BL/6J * CBA/J	MGI:5585184

Genotype
MGI:5319525

cn1

• Allelic Composition: Ago2^tm1.1Tara/Ago2^tm1.1Tara; Tg(KRT14-cre)1Efu/0
• Genetic Background: involves: 129P2/OlaHsd

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

normal phenotype

no abnormal phenotype detected ( J:183622 )

• no developmental defects are detected

Genotype
MGI:5319527

cn2

• Allelic Composition: Ago1^tm1.1Tara/Ago1^tm1.1Tara; Ago2^tm1.1Tara/Ago2^tm1.1Tara; Tg(KRT14-cre)1Efu/0
• Genetic Background: involves: 129P2/OlaHsd

integument

epidermal cyst ( J:183622 )

• evaginating hair follicle cysts in the epidermis at P4

sparse hair ( J:183622 )

abnormal hair follicle morphology ( J:183622 )

• evaginating hair follicle cysts in the epidermis at P4

abnormal hair follicle development ( J:183622 )

• shortened and misangled hair follicle growth at P4

hair follicle degeneration ( J:183622 )

thick epidermis ( J:183622 )

• hyperthickened

abnormal skin physiology ( J:183622 )

• apoptotic cells in the basal epidermis

growth/size/body

epidermal cyst ( J:183622 )

• evaginating hair follicle cysts in the epidermis at P4

Genotype
MGI:4839763

cn3

• Allelic Composition: Ago2^tm1.1Tara/Ago2^tm1.1Tara; Tg(Mx1-cre)1Cgn/0
• Genetic Background: involves: 129P2/OlaHsd * 129S4/SvJaeSor * C57BL/6 * CBA

hematopoietic system

enlarged spleen ( J:165967 )

• in lethally irradiated mice reconstituted with pIpC-treated bone marrow cells

anemia ( J:165967 )

• in lethally irradiated mice reconstituted with pIpC-treated bone marrow cells

abnormal erythrocyte morphology ( J:165967 )

• in lethally irradiated mice reconstituted with pIpC-treated bone marrow cells, red blood cells contain Heinz bodies and hemoglobin precipitates unlike in cells from mice reconstituted with untreated bone marrow

abnormal erythropoiesis ( J:165967 )

• erythroid precursor maturation is severely impaired with erythroid hyperplasia in bone marrow and spleen in lethally irradiated mice reconstituted with pIpC-treated bone marrow cells compared to when untreated bone marrow is used

decreased erythrocyte cell number ( J:165967 )

• in lethally irradiated mice reconstituted with pIpC-treated bone marrow cells

decreased hematocrit ( J:165967 )

• in lethally irradiated mice reconstituted with pIpC-treated bone marrow cells

decreased hemoglobin content ( J:165967 )

• in lethally irradiated mice reconstituted with pIpC-treated bone marrow cells

decreased mean corpuscular hemoglobin ( J:165967 )

• in lethally irradiated mice reconstituted with pIpC-treated bone marrow cells

decreased mean corpuscular volume ( J:165967 )

• in lethally irradiated mice reconstituted with pIpC-treated bone marrow cells

increased red blood cell distribution width ( J:165967 )

• in lethally irradiated mice reconstituted with pIpC-treated bone marrow cells

increased number of Heinz bodies ( J:165967 )

reticulocytosis ( J:165967 )

• in lethally irradiated mice reconstituted with pIpC-treated bone marrow cells

abnormal erythrocyte physiology ( J:165967 )

• in lethally irradiated mice reconstituted with pIpC-treated bone marrow cells

immune system

enlarged spleen ( J:165967 )

• in lethally irradiated mice reconstituted with pIpC-treated bone marrow cells

growth/size/body

enlarged spleen ( J:165967 )

• in lethally irradiated mice reconstituted with pIpC-treated bone marrow cells

Genotype
MGI:5585185

cn4

• Allelic Composition: Ago2^tm1.1Tara/Ago2^tm1.1Tara; Lep^ob/Lep^ob; Tg(Ins2-cre)23Herr/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6J * CBA/J

homeostasis/metabolism

decreased circulating glucose level ( J:210552 )

• random glucose compared with Lep^ob homozygotes

decreased circulating insulin level ( J:210552 )

• compared with Lep^ob homozygotes

increased circulating insulin level ( J:210552 )

• after glucose challenge

improved glucose tolerance ( J:210552 )

• after glucose challenge compared with Lep^ob homozygotes

endocrine/exocrine glands

endocrine/exocrine gland phenotype ( J:210552 )

N • mice exhibit normal numbers of glucagon expressing cells and pancreatic mass

increased pancreatic beta cell mass ( J:210552 )

• not as severe as in Lep^ob homozygotes

increased pancreatic beta cell number ( J:210552 )

• not as severe as in Lep^ob homozygotes

Genotype
MGI:5585184

cn5

• Allelic Composition: Ago2^tm1.1Tara/Ago2^tm1.1Tara; Tg(Ins2-cre)23Herr/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6J * CBA/J

endocrine/exocrine glands

endocrine/exocrine gland phenotype ( J:210552 )

N • mice exhibit normal islet architecture, alpha cell number, total pancreas mass and beta cell cell death

N • mice fed a high fat diet exhibit normal islet morphometry

decreased pancreatic beta cell proliferation ( J:210552 )

abnormal pancreatic beta cell morphology ( J:210552 )

• increased beta cell size and total number of granules

• however, the number of docked granules is normal

decreased pancreatic beta cell mass ( J:210552 )

decreased pancreatic beta cell number ( J:210552 )

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:210552 )

N • mice fed a high fat diet exhibit normal glucose tolerance and energy expenditure

improved glucose tolerance ( J:210552 )

• at 10 weeks without altered insulin sensitivity

cellular

decreased pancreatic beta cell proliferation ( J:210552 )