Phenotypes associated with this allele

• Allele Symbol: Vcan^tm1.1Hwat
• Allele Name: targeted mutation 1.1, Hideto Watanabe
• Allele ID: MGI:4840040
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Vcan^tm1.1Hwat/Vcan^tm1.1Hwat	involves: C57BL/6	MGI:4840049
cn2	Vcan^tm1.1Hwat/Vcan^tm1.1Hwat Tg(Prrx1-cre)1Cjt/0	involves: C57BL/6 * C57BL/6J * SJL/J	MGI:4840048

Genotype
MGI:4840049

hm1

• Allelic Composition: Vcan^tm1.1Hwat/Vcan^tm1.1Hwat
• Genetic Background: involves: C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

normal phenotype

no abnormal phenotype detected ( J:165936 )

• mice are healthy and fertile

Genotype
MGI:4840048

cn2

• Allelic Composition: Vcan^tm1.1Hwat/Vcan^tm1.1Hwat; Tg(Prrx1-cre)1Cjt/0
• Genetic Background: involves: C57BL/6 * C57BL/6J * SJL/J

Limb deformities in Vcan^tm1.1Hwat/Vcan^tm1.1Hwat Tg(Prrx1-cre)1Cjt/0 mice

limbs/digits/tail

abnormal autopod morphology ( J:165936 )

• at E18.5, metatarsus exhibit an aberrant nodule of hypertrophic chondrocytes surrounded by prehypertrophic and proliferative chondrocytes in a concentric pattern without vascular invasion unlike in wild-type mice

• at E18.5, mice exhibit clefting of the metatarsophalangeal joints compared with wild-type mice

abnormal digit morphology ( J:165936 )

• in the hind limb, mice exhibit distortion of digit morphology compared with wild-type mice

• digits contain a nodule of hypertrophic chondrocytes surrounded by proliferative and prehypertrophic chondrocytes unlike in wild-type mice

• the proximal region of proximal phalanges exhibits a cleft unlike in wild-type mice

• at E14.5, no clear joint interzone stripes are observed unlike in wild-type mice

• at E15.5, digits exhibit a broader interzone of small mesenchymal cells compared to in wild-type mice that gives rise to a wedge-shaped or vertical/longitudinal cavity and lacks hypertrophic chondrocytes

• however, digits in the fore limb are normal

abnormal phalanx morphology ( J:165936 )

• as early as 1 week of age, especially the proximal phalanges, in the hind limb but not the fore limb

• the joint surface between phalanges is tilted unlike in wild-type mice

short limbs ( J:165936 )

skeleton

abnormal phalanx morphology ( J:165936 )

• as early as 1 week of age, especially the proximal phalanges, in the hind limb but not the fore limb

• the joint surface between phalanges is tilted unlike in wild-type mice

abnormal chondrocyte physiology ( J:165936 )

• in micromass culture, chondrocyte differentiation within hind limb digits is delayed compared to in wild-type mice