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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Sema4ctm1Matl
targeted mutation 1, Marc Tessier-Lavigne
MGI:4840317
Summary 8 genotypes


Genotype
MGI:4881693
hm1
Allelic
Composition		 Sema4ctm1Matl/Sema4ctm1Matl
Genetic
Background		 B6.129P2-Sema4ctm1Matl
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Sema4ctm1Matl mutation (0 available); any Sema4c mutation (36 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
neonatal lethality, complete penetrance ( J:167973 )
• mice with exencephaly die shortly after first

nervous system
abnormal cerebellar granule cell migration ( J:167973 )
• migration of granule cell precursors (GCPs) out of an explant is reduced compared to in wild-type explants
• however, GCP migration in a transwell assay with stromal cell line-derived factor-1alpha (SDF-1alpha) or brain derived neurotrophic factor (BDNF) is normal
abnormal cerebellar granule layer morphology ( J:167973 )
• 50% of mice exhibit gaps in the granule layer of lobule II unlike wild-type mice
ectopic cerebellar granule cells ( J:167973 )
• 60% of mice exhibit ectopic granule cells in the molecular layer at subpilial positions unlike wild-type mice
abnormal cerebellum lobule morphology ( J:167973 )
• 80% of mice exhibit rostral lobules VIII and IX fusion with disruption of the basal lamina at the site of fusion unlike in wild-type mice
exencephaly ( J:167973 )
• in one third of mice

pigmentation
belly spot ( J:167973 )
• white patches along the ventral midline

integument
belly spot ( J:167973 )
• white patches along the ventral midline

cellular
abnormal cerebellar granule cell migration ( J:167973 )
• migration of granule cell precursors (GCPs) out of an explant is reduced compared to in wild-type explants
• however, GCP migration in a transwell assay with stromal cell line-derived factor-1alpha (SDF-1alpha) or brain derived neurotrophic factor (BDNF) is normal

renal/urinary system
renal/urinary system phenotype ( J:221423 )
N
• mice show normal kidney morphology and a normal response to kidney ischemia/reperfusion injury




Genotype
MGI:4881694
ht2
Allelic
Composition		 Sema4ctm1Matl/Sema4c+
Genetic
Background		 B6.129P2-Sema4ctm1Matl
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Sema4ctm1Matl mutation (0 available); any Sema4c mutation (36 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
nervous system
abnormal cerebellar granule layer morphology ( J:167973 )
• 20% of mice exhibit gaps in the granule layer of lobule II unlike wild-type mice
ectopic cerebellar granule cells ( J:167973 )
• 10% of mice exhibit ectopic granule cells in the molecular layer at subpilial positions unlike wild-type mice
abnormal cerebellum lobule morphology ( J:167973 )
• 27% of mice exhibit rostral lobules VIII and IX fusion unlike in wild-type mice




Genotype
MGI:4881696
cx3
Allelic
Composition		 Plxnb2tm1Matl/Plxnb2tm1Matl
Sema4ctm1Matl/Sema4ctm1Matl
Genetic
Background		 B6.129P2-Sema4ctm1Matl Plxnb2tm1Matl
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Plxnb2tm1Matl mutation (2 available); any Plxnb2 mutation (88 available)
Sema4ctm1Matl mutation (0 available); any Sema4c mutation (36 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
nervous system
abnormal cerebellar granule cell migration ( J:167973 )
• in a transwell assay with stromal cell line-derived factor-1alpha (SDF-1alpha) or brain derived neurotrophic factor (BDNF), granule cell precursors exhibit impaired migration compared with wild-type cells and cells form Sema4ctm1Matl homozygotes
abnormal cerebellar granule layer morphology ( J:167973 )
• the internal granule layer of lobules II and III is disrupted compared to in wild-type mice and more severely disrupted compared to in Sema4ctm1Matl homozygotes

cellular
abnormal cerebellar granule cell migration ( J:167973 )
• in a transwell assay with stromal cell line-derived factor-1alpha (SDF-1alpha) or brain derived neurotrophic factor (BDNF), granule cell precursors exhibit impaired migration compared with wild-type cells and cells form Sema4ctm1Matl homozygotes




Genotype
MGI:4881697
cx4
Allelic
Composition		 Plxnb2tm1Matl/Plxnb2+
Sema4ctm1Matl/Sema4ctm1Matl
Genetic
Background		 B6.129P2-Sema4ctm1Matl Plxnb2tm1Matl
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Plxnb2tm1Matl mutation (2 available); any Plxnb2 mutation (88 available)
Sema4ctm1Matl mutation (0 available); any Sema4c mutation (36 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
nervous system
abnormal cerebellar granule layer morphology ( J:167973 )
• mice exhibit a gap in the lobule X unlike wild-type mice and Sema4ctm1Matl homozygotes




Genotype
MGI:4881700
cx5
Allelic
Composition		 Sema4ctm1Matl/Sema4c+
Sema4gtm1Kik/Sema4g+
Genetic
Background		 B6.Cg-Sema4ctm1Matl Sema4gtm1Kik
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Sema4ctm1Matl mutation (0 available); any Sema4c mutation (36 available)
Sema4gtm1Kik mutation (0 available); any Sema4g mutation (33 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
nervous system
abnormal cerebellar granule layer morphology ( J:167973 )
• 40% of mice exhibit gaps in the granule layer of lobule II unlike wild-type mice
ectopic cerebellar granule cells ( J:167973 )
• 40% of mice exhibit ectopic granule cells in the molecular layer at subpilial positions unlike wild-type mice
abnormal cerebellum lobule morphology ( J:167973 )
• 69% of mice exhibit rostral lobules VIII and IX fusion unlike in wild-type mice




Genotype
MGI:4881701
cx6
Allelic
Composition		 Sema4ctm1Matl/Sema4ctm1Matl
Sema4gtm1Kik/Sema4g+
Genetic
Background		 B6.Cg-Sema4ctm1Matl Sema4gtm1Kik
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Sema4ctm1Matl mutation (0 available); any Sema4c mutation (36 available)
Sema4gtm1Kik mutation (0 available); any Sema4g mutation (33 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
nervous system
abnormal cerebellar granule layer morphology ( J:167973 )
• 60% of mice exhibit gaps in the granule layer of lobule II and X unlike wild-type mice
ectopic cerebellar granule cells ( J:167973 )
• 80% of mice exhibit ectopic granule cells in the molecular layer at subpilial positions unlike wild-type mice
abnormal cerebellum lobule morphology ( J:167973 )
• all mice exhibit rostral lobules VIII and IX fusion unlike in wild-type mice




Genotype
MGI:4881702
cx7
Allelic
Composition		 Sema4ctm1Matl/Sema4c+
Sema4gtm1Kik/Sema4gtm1Kik
Genetic
Background		 B6.Cg-Sema4ctm1Matl Sema4gtm1Kik
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Sema4ctm1Matl mutation (0 available); any Sema4c mutation (36 available)
Sema4gtm1Kik mutation (0 available); any Sema4g mutation (33 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
nervous system
abnormal cerebellar granule layer morphology ( J:167973 )
• 50% of mice exhibit gaps in the granule layer of lobule II unlike wild-type mice
ectopic cerebellar granule cells ( J:167973 )
• 60% of mice exhibit ectopic granule cells in the molecular layer at subpilial positions unlike wild-type mice
abnormal cerebellum lobule morphology ( J:167973 )
• 69% of mice exhibit rostral lobules VIII and IX fusion unlike in wild-type mice




Genotype
MGI:4881703
cx8
Allelic
Composition		 Sema4ctm1Matl/Sema4ctm1Matl
Sema4gtm1Kik/Sema4gtm1Kik
Genetic
Background		 B6.Cg-Sema4ctm1Matl Sema4gtm1Kik
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Sema4ctm1Matl mutation (0 available); any Sema4c mutation (36 available)
Sema4gtm1Kik mutation (0 available); any Sema4g mutation (33 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
nervous system
abnormal cerebellar granule layer morphology ( J:167973 )
• 67% of mice exhibit gaps in the granule layer of lobule II unlike wild-type mice
• 75% of mice exhibit gaps in the granule layer of lobule X unlike wild-type mice
ectopic cerebellar granule cells ( J:167973 )
• all mice exhibit ectopic granule cells in the molecular layer at subpilial positions unlike wild-type mice
abnormal cerebellum lobule morphology ( J:167973 )
• all mice exhibit rostral lobules VIII and IX fusion unlike in wild-type mice




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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory