Phenotypes associated with this allele

• Allele Symbol: Zfp521^tm1Ngc
• Allele Name: targeted mutation 1, Neal G Copeland
• Allele ID: MGI:4840366
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Zfp521^tm1Ngc/Zfp521^tm1Ngc Tg(Col2a1-cre)#Amc/0 Tg(Col2a1-PTHR1*H223R)AHju/0	involves: C57BL/6	MGI:5294939
cn2	Zfp521^tm1Ngc/Zfp521^tm1Ngc Tg(Col2a1-cre)#Amc/0	Not Specified	MGI:4840376

Genotype
MGI:5294939

cn1

• Allelic Composition: Zfp521^tm1Ngc/Zfp521^tm1Ngc; Tg(Col2a1-cre)#Amc/0; Tg(Col2a1-PTHR1*H223R)AHju/0
• Genetic Background: involves: C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

skeleton

skeleton phenotype ( J:175572 )

N • mice exhibit normal bone length

N • tibial growth plate of mutants is similar to wild-type, indicating rescue of the chondrocyte differentiation delay seen in single Tg(Col2a1-PTHR1*H223R)AHju/0 mice and improvement in endochondral bone formation

Genotype
MGI:4840376

cn2

• Allelic Composition: Zfp521^tm1Ngc/Zfp521^tm1Ngc; Tg(Col2a1-cre)#Amc/0
• Genetic Background: Not Specified

skeleton

abnormal long bone epiphyseal plate morphology ( J:166058 )

• the transition to the hypetrophic zone is closer to the epiphysis with fewer chondrocytes of increased cellular height and reduced matrix compared with wild-type mice

abnormal long bone epiphyseal plate proliferative zone ( J:166058 )

• reduced

decreased width of hypertrophic chondrocyte zone ( J:166058 )

decreased long bone epiphyseal plate size ( J:166058 )

• with a reduction in the number of cell columns and cells per column

decreased length of long bones ( J:166058 , J:175572 )

small thoracic cage ( J:166058 )

small vertebrae ( J:166058 )

abnormal bone structure ( J:166058 )

• bones formed by endochondral ossification are smaller than in wild-type mice

• however, craniofacial structures that develop by intramembranous ossification and the cranium base synchondroses are normal

abnormal bone mineralization ( J:166058 )

• extracellular matrix mineralization is premature compared to in wild-type mice

abnormal chondrocyte physiology ( J:166058 , J:175572 )

• growth plate chondrocytes exhibit reduced proliferation compared to in wild-type mice (J:166058)

• chondrocyte progression to hypertrophy is slowed compared to in wild-type mice (J:166058)

• chondrocyte apoptosis is increased compared to in wild-type mice (J:166058)

growth/size/body

proportional dwarf ( J:166058 )

postnatal growth retardation ( J:166058 , J:175572 )

• beginning at 1 to 2 weeks of age and persisting through 8 and 12 weeks (J:166058)