Phenotypes associated with this allele

• Allele Symbol: Ep400^tm3.1Fuku
• Allele Name: targeted mutation 3.1, Rikiro Fukunaga
• Allele ID: MGI:4845875
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Ep400^tm3.1Fuku/Ep400^tm3.1Fuku	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * FVB/N	MGI:4845884
cn2	Ep400^tm3Fuku/Ep400^tm3.1Fuku Tg(Mx1-cre)1Cgn/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA	MGI:4845885

Genotype
MGI:4845884

hm1

• Allelic Composition: Ep400^tm3.1Fuku/Ep400^tm3.1Fuku
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * FVB/N

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

prenatal lethality, complete penetrance ( J:166397 )

• no homozygous mice were born from intercrosses of heterozygous mice

Genotype
MGI:4845885

cn2

• Allelic Composition: Ep400^tm3Fuku/Ep400^tm3.1Fuku; Tg(Mx1-cre)1Cgn/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA

Loss of bone marrow hematopoietic cells in Ep400^tm3Fuku/Ep400^tm3.1Fuku Tg(Mx1-cre)1Cgn/0 mice

mortality/aging

premature death ( J:166397 )

• pIpC-treated mice die within 14 days unlike similarly treated heterozygous mice

hematopoietic system

abnormal definitive hematopoiesis ( J:166397 )

• pIpC-treated mice exhibit a reduction in nucleated hematopoietic cells and an increase in CD71-Ter119^high in the bone marrow cavity compared with similarly treated heterozygous mice

abnormal bone marrow cell morphology/development ( J:166397 )

• bone marrow cells from pIpC-treated mice exhibit reduced colony formation in response to IFN-beta treatment unlike similarly treated cells from heterozygous mice

• however, colony formation in the absence of IFN-beta is normal

decreased bone marrow cell number ( J:166397 )

• pIpC-treated mice exhibit a reduction of nucleated cells, B220 B+ cells, and Ter119+ erythroid cells in the bone marrow compared with similarly treated heterozygous mice

decreased erythroid progenitor cell number ( J:166397 )

• in pIpC-treated mice

abnormal megakaryocyte progenitor cell morphology ( J:166397 )

• pIpC-treated mice exhibit a reduction in Mac1+Gr-1^mid-lo monocyte/macrophage lineage and Mac-1+Gr-1^hi granulocyte lineage compared with similarly treated heterozygous mice

decreased hematopoietic stem cell number ( J:166397 )

• in pIpC-treated mice

abnormal bone marrow cell physiology ( J:166397 )

• pIpC-treated mice exhibit an increase in apoptosis compared to in similarly treated heterozygous mice