Phenotypes associated with this allele

• Allele Symbol: Dock4^{tm1(NCOM)Mfgc}
• Allele Name: targeted mutation 1, Mammalian Functional Genomics Centre
• Allele ID: MGI:4880372
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Dock4^tm1(NCOM)Mfgc/Dock4^tm1(NCOM)Mfgc	involves: C57BL/6J * C57BL/6NCrl	MGI:5774776
ht2	Dock4^tm1(NCOM)Mfgc/Dock4^+	involves: C57BL/6J * C57BL/6NCrl	MGI:5774777

Genotype
MGI:5774776

hm1

• Allelic Composition: Dock4^{tm1(NCOM)Mfgc}/Dock4^{tm1(NCOM)Mfgc}
• Genetic Background: involves: C57BL/6J * C57BL/6NCrl
• Cell Lines: N01793P1_C_109W_G2

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality, complete penetrance ( J:224385 )

• no homozygotes are recovered at E7.5

Genotype
MGI:5774777

ht2

• Allelic Composition: Dock4^{tm1(NCOM)Mfgc}/Dock4⁺
• Genetic Background: involves: C57BL/6J * C57BL/6NCrl
• Cell Lines: N01793P1_C_109W_G2

Dock4^{tm1(NCOM)Mfgc}/Dock4⁺ embryos show an increase in the frequency of blood vessels with small lumen width and a decrease in the frequency of large lumen width in the brain

cardiovascular system

abnormal tumor vascularization ( J:224385 )

• following implantation of a syngeneic breast cancer cell line (EO771) into the striatum of 9-10 week old heterozygotes, tumors show a reduction in blood vessel lumen width with 21% of vessels having lumens of larger caliber (>35 um) relative to ~38% in control tumors

abnormal blood vessel lumen formation ( J:224385 )

• during developmental angiogenesis, E13.5 heterozygotes show only 9% of blood vessels with lumens of relatively large caliber (>20 um) in the brain parenchyma relative to ~31% in wild-type controls

• at E13.5, heterozygotes show a significant reduction in average blood vessel lumen width in the brain (15.1 um vs 18.6 um) relative to wild-type controls

• however, no differences in pericyte coverage of blood vessels are detected in the brain at E13.5

• no differences are detected in the lumen caliber of the aortas at E13.5

neoplasm

abnormal tumor vascularization ( J:224385 )

• following implantation of a syngeneic breast cancer cell line (EO771) into the striatum of 9-10 week old heterozygotes, tumors show a reduction in blood vessel lumen width with 21% of vessels having lumens of larger caliber (>35 um) relative to ~38% in control tumors