Analysis Tools
cellular
|
• osteoblast colony forming units from mice with a paternally inherited mutant allele are smaller and contain cells with fewer contacts and less dense packing
|
|
• in culture hematopoietic progenitors from mice with a paternally inherited mutant allele produce fewer large (greater than 10 nuclei) osteoclasts
• however, mutant cells transplanted into wild-type mice are able to form normal numbers of large osteoclasts
|
|
• only mice inheriting the mutant allele paternally show abnormal phenotypes
|
growth/size/body
|
• growth retardation is detectable as early as E10.5 in mice with a paternally inherited mutant allele
|
|
• about a 30% reduction in birth weight in mice with a paternally inherited mutant allele
|
|
• mice with a paternally inherited mutant allele remain growth retarded at 12 weeks of age
|
|
• growth retardation becomes more pronounced between E13.5 and E17.5 in mice with a paternally inherited mutant allele
|
skeleton
|
• thinner and shorter bones in mice with a paternally inherited mutant allele
|
|
• the bone is thinner but cortical bone thickness is increased and marrow cavity size is decreased in mice with a paternally inherited mutant allele
|
|
• marrow cavity size is decreased in mice with a paternally inherited mutant allele
|
|
• at 2, 3 and 12 months of age in mice with a paternally inherited mutant allele
|
|
• the lamellar structure of the cortical bone is irregular and poorly formed, with poorly aligned osteocytes and incompletely formed lacunae in mice with a paternally inherited mutant allele
|
|
• in mice with a paternally inherited mutant allele
|
|
• in adult mice with a paternally inherited mutant allele
|
|
• dense and disorganized trabeculae in the femurs of mice with a paternally inherited mutant allele at 3 months of age
|
|
• adult mice with a paternally inherited mutant allele have reduced numbers of mesenchymal stem cells and osteoprogenitors
|
|
• osteoblast colony forming units from mice with a paternally inherited mutant allele are smaller and contain cells with fewer contacts and less dense packing
|
|
• in culture hematopoietic progenitors from mice with a paternally inherited mutant allele produce fewer large (greater than 10 nuclei) osteoclasts
• however, mutant cells transplanted into wild-type mice are able to form normal numbers of large osteoclasts
|
|
• the number of chondrocyte colonies formed in E11.5 limb bud mesenchymal cell cultures from mice with a paternally inherited mutant allele is reduced and colonies that do form do so more slowly and have a less mature phenotype
|
|
• in mice with a paternally inherited mutant allele
|
embryo
|
• growth retardation is detectable as early as E10.5 in mice with a paternally inherited mutant allele
|
limbs/digits/tail
|
• the bone is thinner but cortical bone thickness is increased and marrow cavity size is decreased in mice with a paternally inherited mutant allele
|
hematopoietic system
|
• in culture hematopoietic progenitors from mice with a paternally inherited mutant allele produce fewer large (greater than 10 nuclei) osteoclasts
• however, mutant cells transplanted into wild-type mice are able to form normal numbers of large osteoclasts
|
immune system
|
• in culture hematopoietic progenitors from mice with a paternally inherited mutant allele produce fewer large (greater than 10 nuclei) osteoclasts
• however, mutant cells transplanted into wild-type mice are able to form normal numbers of large osteoclasts
|
