Phenotypes associated with this allele

• Allele Symbol: Irx3^tm1Hui
• Allele Name: targeted mutation 1, Chi-chung Hui
• Allele ID: MGI:4882110
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Irx3^tm1Hui/Irx3^tm1Hui	involves: 129S1/Sv * 129X1/SvJ	MGI:5616242
hm2	Irx3^tm1Hui/Irx3^tm1Hui	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:5292475
cx3	Gja5^tm1Lumi/Gja5^+ Irx3^tm1Hui/Irx3^tm1Hui	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:5292476

Genotype
MGI:5616242

hm1

• Allelic Composition: Irx3^tm1Hui/Irx3^tm1Hui
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

growth/size/body

decreased body weight ( J:208887 )

• 25-30% reduction in body weight compared to control littermates

• difference is more pronounced when mice are on a high fat diet, with mutant mice showing no weight gain compared to a 63% increase in controls

decreased susceptibility to diet-induced obesity ( J:208887 )

• resistant to high fat diet induced obesity

adipose tissue

decreased fat cell size ( J:208887 )

• reduced adipocyte size in mice on a high fat diet compared to wild-type controls

decreased percent body fat/body weight ( J:208887 )

• in mice on a normal chow or high fat diet compared to wild-type controls

• marked reduction in adiposity with smaller fat depots as well as reduced adipocyte size in mice on a high fat diet compared to wild-type controls

abnormal white adipose tissue physiology ( J:208887 )

• expression analysis indicates browning of white adipose

homeostasis/metabolism

increased energy expenditure ( J:208887 )

• higher energy expenditure and upregulation of brown adipose markers

decreased susceptibility to diet-induced obesity ( J:208887 )

• resistant to high fat diet induced obesity

improved glucose tolerance ( J:208887 )

• resistant to high fat diet and age induced glucose intolerance

increased insulin sensitivity ( J:208887 )

• resistant to high fat diet and age induced insulin resistance

liver/biliary system

decreased susceptibility to diet-induced hepatic steatosis ( J:208887 )

• resistant to high fat diet induced hepatic steatosis

Genotype
MGI:5292475

hm2

• Allelic Composition: Irx3^tm1Hui/Irx3^tm1Hui
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6

cardiovascular system

abnormal atrioventricular bundle conduction ( J:175992 )

• increase of the conduction time between the His bundle and ventricles

• right bundle branch block

abnormal heart electrocardiography waveform feature ( J:175992 )

• display ventricular activation defects at 2 weeks of age

abnormal QRS complex ( J:175992 )

• right axis deviation, detected as a negative QRS deflection in lead I combined with a positive deflection in lead II, is observed in 70% of mice

prolonged QRS complex duration ( J:175992 )

abnormal R wave ( J:175992 )

• notched R wave

Genotype
MGI:5292476

cx3

• Allelic Composition: Gja5^tm1Lumi/Gja5⁺; Irx3^tm1Hui/Irx3^tm1Hui
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6

cardiovascular system

abnormal impulse conducting system conduction ( J:175992 )

• significantly diminished conduction velocity through the conduction fibers