All Phenotypes Tg(Pmch-cre)1Lowl MGI Mouse

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Phenotypes associated with this allele

Allele Symbol
Allele Name
Allele ID

Tg(Pmch-cre)1Lowl
transgene insertion 1, Bradford B Lowell
MGI:4887235

Summary

3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Gt(ROSA)26Sor^{tm1(CAG-Kcnj11*,-GFP)Nich}/? Tg(CAG-Bgeo/GFP)21Lbe/0 Tg(Pmch-cre)1Lowl/0	involves: 129S1/Sv * 129S4/SvJaeSor * 129X1/SvJ * C57BL/6 * FVB/NJ	MGI:4887238
cn2	Ucp2^tm2.1Lowl/Ucp2^tm2.1Lowl Tg(CAG-Bgeo/GFP)21Lbe/0 Tg(Pmch-cre)1Lowl/0	involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ * C57BL/6 * FVB/NJ	MGI:4887239
cn3	Ucp2^tm2.1Lowl/Ucp2^tm2.1Lowl Gt(ROSA)26Sor^{tm1(CAG-Kcnj11*,-GFP)Nich}/? Tg(Pmch-cre)1Lowl/0	involves: 129S4/SvJaeSor * 129S6/SvEvTac * C57BL/6 * FVB/NJ	MGI:4887257

Allelic Composition	Gt(ROSA)26Sor^{tm1(CAG-Kcnj11*,-GFP)Nich}/? Tg(CAG-Bgeo/GFP)21Lbe/0 Tg(Pmch-cre)1Lowl/0
Genetic Background	involves: 129S1/Sv * 129S4/SvJaeSor * 129X1/SvJ * C57BL/6 * FVB/NJ

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gt(ROSA)26Sor^{tm1(CAG-Kcnj11*,-GFP)Nich} mutation (0 available); any Gt(ROSA)26Sor mutation (993 available)
Tg(CAG-Bgeo/GFP)21Lbe mutation (2 available)
Tg(Pmch-cre)1Lowl mutation (2 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:167906 )

N	• 8-week-old male mutants do not show differences in body weight, daily food intake, ad libitum-fed blood glucose levels, or overnight fasted blood glucose levels

impaired glucose tolerance ( J:167906 )

• 8-week-old males display impaired glucose tolerance (elevated blood glucose after injection of glucose at 2 g/kg body weight) compared to control littermates

• no alterations in pancreatic beta cells is observed due to ectopic cre expression resulting in mutant Kcnj11 expression in beta cells

nervous system

abnormal single cell response ( J:167906 )

• only about 14% of Pmch neurons show spontaneous action potentials at 5 mM glucose in brain slice preparations compared to 30% of neurons in wild-type preparations, indicating disrupted glucose-sensing

• membrane resistance of Pmch neurons is significantly reduced relative to wild-type neurons at 5 mM glucose

• almost all neurons do not hyperpolarize when extracellular glucose is reduced from 5 to 1 mM whereas around 70% of control neurons hyperpolarize under these conditions, indicating disrupted glucose-sensing

• glucose-induced depolarization is abrogated in Pmch neurons, but is readily observed in control Pmch neurons, indicating disrupted glucose-sensing

Allelic Composition	Ucp2^tm2.1Lowl/Ucp2^tm2.1Lowl Tg(CAG-Bgeo/GFP)21Lbe/0 Tg(Pmch-cre)1Lowl/0
Genetic Background	involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ * C57BL/6 * FVB/NJ

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(CAG-Bgeo/GFP)21Lbe mutation (2 available)
Tg(Pmch-cre)1Lowl mutation (2 available)
Ucp2^tm2.1Lowl mutation (1 available); any Ucp2 mutation (41 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:167906 )

N	• 8-week-old male mutants do not show differences in body weight or daily food intake

decreased circulating glucose level ( J:167906 )

• ad libitum-fed and overnight fasted blood glucose levels of 8-week-old male mutants are significantly reduced relative to controls

decreased circulating insulin level ( J:167906 )

• during glucose tolerance tests, insulin levels are decreased relative to controls, suggesting that decreased glucose levels are not the result of increased insulin secretion

improved glucose tolerance ( J:167906 )

• 8-week-old males display improved glucose tolerance (after injection of glucose at 2 g/kg body weight), with a significantly reduced rise in blood glucose levels relative to controls

• no alterations in pancreatic beta cells is observed due to ectopic cre expression resulting in Ucp2 deletion in beta cells

nervous system

abnormal single cell response ( J:167906 )

• about 44% of Pmch neurons fire spontaneously at 5 mM glucose, increased from around 30% of control neurons

• membrane potential of Pmch neurons displays enhanced glucose-induced depolarization in response to different glucose concentrations (left-shifted dose-response curve to glucose)

Allelic Composition	Ucp2^tm2.1Lowl/Ucp2^tm2.1Lowl Gt(ROSA)26Sor^{tm1(CAG-Kcnj11*,-GFP)Nich}/? Tg(Pmch-cre)1Lowl/0
Genetic Background	involves: 129S4/SvJaeSor * 129S6/SvEvTac * C57BL/6 * FVB/NJ

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gt(ROSA)26Sor^{tm1(CAG-Kcnj11*,-GFP)Nich} mutation (0 available); any Gt(ROSA)26Sor mutation (993 available)
Tg(Pmch-cre)1Lowl mutation (2 available)
Ucp2^tm2.1Lowl mutation (1 available); any Ucp2 mutation (41 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

homeostasis/metabolism

impaired glucose tolerance ( J:167906 )

• 8-week-old males display impaired glucose tolerance (elevated blood glucose at times after injection of glucose at 2 g/kg body weight) compared to control littermates; blood glucose is elevated significantly compared to mice lacking Ucp2 in Pmch neurons or to wild-type controls, indicating that mutant Kcnj11 (Kir6.2) expression blocks glucose-lowering effects of Ucp2 deficiency

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