Phenotypes associated with this allele

• Allele Symbol: Tg(Tcfap2a-cre)1Will
• Allele Name: transgene insertion 1, Trevor Williams
• Allele ID: MGI:4887352
• Summary: 8 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Pbx1^tm1Koss/Pbx1^tm1Koss Pbx3^tm1Mlc/Pbx3^+ Tg(Tcfap2a-cre)1Will/0	involves: 129P2/OlaHsd	MGI:5305926
cn2	Pbx1^tm1Koss/Pbx1^tm1Koss Pbx2^tm1Mlc/Pbx2^+ Tg(Tcfap2a-cre)1Will/0	involves: 129P2/OlaHsd * 129S/Sv	MGI:5305925
cn3	Gt(ROSA)26Sor^tm2(Wnt1/GFP)Amc/Gt(ROSA)26Sor^+ Pbx1^tm1Koss/Pbx1^tm1Koss Pbx2^tm1Mlc/Pbx2^+ Tg(Tcfap2a-cre)1Will/0	involves: 129P2/OlaHsd * 129S/Sv * 129X1/SvJ	MGI:5305934
cn4	Ctnnb1^tm2Kem/Ctnnb1^tm2Kem Tg(Tcfap2a-cre)1Will/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:4887453
cn5	Esrp1^tm1.1Rpc/Esrp1^tm1.1Rpc Tg(Tcfap2a-cre)1Will/0	involves: 129S4/SvJae * C57BL/6	MGI:7437689
cn6	Ctnnd1^tm1Abre/Ctnnd1^tm1Abre Tg(Tcfap2a-cre)1Will/0	involves: 129S6/SvEvTac	MGI:7345550
cn7	Ctnnd1^tm1Abre/Ctnnd1^+ Tg(Tcfap2a-cre)1Will/0	involves: 129S6/SvEvTac	MGI:7345551
cn8	Ctnnb1^tm1Mmt/Ctnnb1^+ Tg(Tcfap2a-cre)1Will/0	involves: 129X1/SvJ * C57BL/6	MGI:4887452

Genotype
MGI:5305926

cn1

• Allelic Composition: Pbx1^tm1Koss/Pbx1^tm1Koss; Pbx3^tm1Mlc/Pbx3⁺; Tg(Tcfap2a-cre)1Will/0
• Genetic Background: involves: 129P2/OlaHsd

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

craniofacial

cleft upper lip ( J:178316 )

• cleft lip and/or palate

cleft palate ( J:178316 )

• cleft lip and/or palate

digestive/alimentary system

cleft palate ( J:178316 )

• cleft lip and/or palate

growth/size/body

cleft upper lip ( J:178316 )

• cleft lip and/or palate

cleft palate ( J:178316 )

• cleft lip and/or palate

Genotype
MGI:5305925

cn2

• Allelic Composition: Pbx1^tm1Koss/Pbx1^tm1Koss; Pbx2^tm1Mlc/Pbx2⁺; Tg(Tcfap2a-cre)1Will/0
• Genetic Background: involves: 129P2/OlaHsd * 129S/Sv

craniofacial

cleft upper lip ( J:178316 )

• cleft lip and/or palate

cleft palate ( J:178316 )

• cleft lip and/or palate

digestive/alimentary system

cleft palate ( J:178316 )

• cleft lip and/or palate

growth/size/body

cleft upper lip ( J:178316 )

• cleft lip and/or palate

cleft palate ( J:178316 )

• cleft lip and/or palate

Genotype
MGI:5305934

cn3

• Allelic Composition: Gt(ROSA)26Sor^{tm2(Wnt1/GFP)Amc}/Gt(ROSA)26Sor⁺; Pbx1^tm1Koss/Pbx1^tm1Koss; Pbx2^tm1Mlc/Pbx2⁺; Tg(Tcfap2a-cre)1Will/0
• Genetic Background: involves: 129P2/OlaHsd * 129S/Sv * 129X1/SvJ

craniofacial

craniofacial phenotype ( J:178316 )

N • the cleft lip phenotype observed in Pbx1^tm1.1Koss/Pbx1^tm1.1Koss Pbx2^tm1Mlc/Pbx2⁺ Tg(Tcfap2a*-cre)1Will is rescued

Genotype
MGI:4887453

cn4

• Allelic Composition: Ctnnb1^tm2Kem/Ctnnb1^tm2Kem; Tg(Tcfap2a-cre)1Will/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6

mortality/aging

neonatal lethality, complete penetrance ( J:168016 )

• mutants survive until birth

craniofacial

abnormal neurocranium morphology ( J:168016 )

• all bones are present, but with minor defects

fusion of basioccipital and basisphenoid bone ( J:168016 )

• bones are connected by calcified tissues to form a trabecular basal plate, in contrast to being separated by cartilage as in wild-type embryos

absent hyoid bone ( J:168016 )

• ossifying center of the hyoid bone is missing at E18.5

absent mandible ( J:168016 )

abnormal maxillary zygomatic process morphology ( J:168016 )

• only a fragment of the zygomatic process of the maxilla is found at E18.5

absent middle ear ossicles ( J:168016 )

narrow frontonasal prominence ( J:168016 )

• frontonasal prominence is narrowed, producing a protuberance with a beak-like appearance instead of a normal muzzle

abnormal lateral nasal prominence morphology ( J:168016 )

• hypoplasia of the lateral nasal prominence

abnormal mandibular prominence morphology ( J:168016 )

• the mandibular prominences fuse at the midline, but are severely underdeveloped at E11.5 and 12.5

abnormal medial nasal prominence morphology ( J:168016 )

• hypoplasia of the medial nasal prominence

abnormal nasal placode morphology ( J:168016 )

• underdeveloped in mutants, consistent with hypoplasia of the medial and lateral nasal prominences

abnormal facial morphology ( J:168016 )

• by E10.5 embyros exhibit a hypoplastic facial morphology, more characteristic of E9.5 embryos

abnormal maxillary shelf morphology ( J:168016 )

• part of the palatal processes of the maxilla are present in E18.5 embryos

abnormal face development ( J:168016 )

• all embryos display hypoplasia of the facial prominences

• at E9.0 facial prominences are comparable to wild-type; shortly after, facial prominences fail to grow and by E10.5 embyros exhibit a hypoplastic facial morphology, more characteristic of embryos at E9.5

• the groove overlying the lamina terminalis of forebrain which normally disappears around E10.5 is still present at E11.5

• increased cell death is observed at E10.5 in the developing facial region compared to controls

skeleton

abnormal neurocranium morphology ( J:168016 )

• all bones are present, but with minor defects

fusion of basioccipital and basisphenoid bone ( J:168016 )

• bones are connected by calcified tissues to form a trabecular basal plate, in contrast to being separated by cartilage as in wild-type embryos

absent hyoid bone ( J:168016 )

• ossifying center of the hyoid bone is missing at E18.5

absent mandible ( J:168016 )

abnormal maxillary shelf morphology ( J:168016 )

• part of the palatal processes of the maxilla are present in E18.5 embryos

abnormal maxillary zygomatic process morphology ( J:168016 )

• only a fragment of the zygomatic process of the maxilla is found at E18.5

absent middle ear ossicles ( J:168016 )

hearing/vestibular/ear

absent middle ear ossicles ( J:168016 )

abnormal tympanic ring morphology ( J:168016 )

• most of the ectotympanic bone is missing in mutants at E18.5

vision/eye

abnormal lens development ( J:168016 )

• some defects in lens formation are suggested

abnormal optic eminence morphology ( J:168016 )

• at E10.5 the optic eminence is reduced, as well as the corneal endoderm

absent eyelids ( J:168016 )

• the eyelids fail to form during development

digestive/alimentary system

abnormal maxillary shelf morphology ( J:168016 )

• part of the palatal processes of the maxilla are present in E18.5 embryos

respiratory system

abnormal nasal placode morphology ( J:168016 )

• underdeveloped in mutants, consistent with hypoplasia of the medial and lateral nasal prominences

taste/olfaction

abnormal nasal placode morphology ( J:168016 )

• underdeveloped in mutants, consistent with hypoplasia of the medial and lateral nasal prominences

growth/size/body

abnormal facial morphology ( J:168016 )

• by E10.5 embyros exhibit a hypoplastic facial morphology, more characteristic of E9.5 embryos

abnormal maxillary shelf morphology ( J:168016 )

• part of the palatal processes of the maxilla are present in E18.5 embryos

abnormal face development ( J:168016 )

• all embryos display hypoplasia of the facial prominences

• at E9.0 facial prominences are comparable to wild-type; shortly after, facial prominences fail to grow and by E10.5 embyros exhibit a hypoplastic facial morphology, more characteristic of embryos at E9.5

• the groove overlying the lamina terminalis of forebrain which normally disappears around E10.5 is still present at E11.5

• increased cell death is observed at E10.5 in the developing facial region compared to controls

Genotype
MGI:7437689

cn5

• Allelic Composition: Esrp1^tm1.1Rpc/Esrp1^tm1.1Rpc; Tg(Tcfap2a-cre)1Will/0
• Genetic Background: involves: 129S4/SvJae * C57BL/6

craniofacial

small premaxilla ( J:293624 )

• underdeveloped with a poor connection to the maxilla and extend out in front of the face

bilateral cleft upper lip ( J:293624 )

• bilateral cleft lip/palate at E18.5

• failure of the lip processes to come together to form a midline philtrum

abnormal palate morphology ( J:293624 )

maxillary shelf hypoplasia ( J:293624 )

• similar to palatine bones

palatine bone hypoplasia ( J:293624 )

• hypoplastic and located to the side

cleft primary palate ( J:293624 )

• bilateral cleft of the primary palate at E18.5

cleft secondary palate ( J:293624 )

• complete cleft

bilateral cleft palate ( J:293624 )

• bilateral cleft lip/palate at E18.5

skeleton

maxillary shelf hypoplasia ( J:293624 )

• similar to palatine bones

small premaxilla ( J:293624 )

• underdeveloped with a poor connection to the maxilla and extend out in front of the face

palatine bone hypoplasia ( J:293624 )

• hypoplastic and located to the side

digestive/alimentary system

abnormal palate morphology ( J:293624 )

maxillary shelf hypoplasia ( J:293624 )

• similar to palatine bones

palatine bone hypoplasia ( J:293624 )

• hypoplastic and located to the side

cleft primary palate ( J:293624 )

• bilateral cleft of the primary palate at E18.5

cleft secondary palate ( J:293624 )

• complete cleft

bilateral cleft palate ( J:293624 )

• bilateral cleft lip/palate at E18.5

growth/size/body

bilateral cleft upper lip ( J:293624 )

• bilateral cleft lip/palate at E18.5

• failure of the lip processes to come together to form a midline philtrum

abnormal palate morphology ( J:293624 )

maxillary shelf hypoplasia ( J:293624 )

• similar to palatine bones

palatine bone hypoplasia ( J:293624 )

• hypoplastic and located to the side

cleft primary palate ( J:293624 )

• bilateral cleft of the primary palate at E18.5

cleft secondary palate ( J:293624 )

• complete cleft

bilateral cleft palate ( J:293624 )

• bilateral cleft lip/palate at E18.5

Genotype
MGI:7345550

cn6

• Allelic Composition: Ctnnd1^tm1Abre/Ctnnd1^tm1Abre; Tg(Tcfap2a-cre)1Will/0
• Genetic Background: involves: 129S6/SvEvTac

craniofacial

abnormal maxillary prominence morphology ( J:268949 )

• at E11.5, some non-cleft presenting embryos show a delay in the medial growth of the maxillary prominences

facial asymmetry ( J:268949 )

• approximately half of the non-cleft presenting embryos show obvious nasal airway asymmetry

oral cleft ( J:268949 )

• at E10.5-E18.5, ~47% of embryos exhibit a spectrum of overt clefts including: unilateral cleft lip only; unilateral cleft lip and cleft palate; bilateral cleft lip and cleft palate; or cleft secondary palate only

cleft upper lip ( J:268949 )

• unilateral cleft lip only, unilateral cleft lip and cleft palate, and bilateral cleft lip and cleft palate are observed

cleft palate ( J:268949 )

• unilateral cleft lip and cleft palate as well as bilateral cleft lip and cleft palate are observed

cleft secondary palate ( J:268949 )

• cleft secondary palate only is also observed

bilateral cleft palate ( J:268949 )

unilateral cleft palate ( J:268949 )

abnormal external nares morphology ( J:268949 )

• non-cleft presenting embryos exhibit dysmorphic nares

abnormal nasal tip morphology ( J:268949 )

• non-cleft presenting embryos exhibit dysmorphic nasal tips

growth/size/body

facial asymmetry ( J:268949 )

• approximately half of the non-cleft presenting embryos show obvious nasal airway asymmetry

oral cleft ( J:268949 )

• at E10.5-E18.5, ~47% of embryos exhibit a spectrum of overt clefts including: unilateral cleft lip only; unilateral cleft lip and cleft palate; bilateral cleft lip and cleft palate; or cleft secondary palate only

cleft upper lip ( J:268949 )

• unilateral cleft lip only, unilateral cleft lip and cleft palate, and bilateral cleft lip and cleft palate are observed

cleft palate ( J:268949 )

• unilateral cleft lip and cleft palate as well as bilateral cleft lip and cleft palate are observed

cleft secondary palate ( J:268949 )

• cleft secondary palate only is also observed

bilateral cleft palate ( J:268949 )

unilateral cleft palate ( J:268949 )

abnormal external nares morphology ( J:268949 )

• non-cleft presenting embryos exhibit dysmorphic nares

abnormal nasal tip morphology ( J:268949 )

• non-cleft presenting embryos exhibit dysmorphic nasal tips

digestive/alimentary system

cleft palate ( J:268949 )

• unilateral cleft lip and cleft palate as well as bilateral cleft lip and cleft palate are observed

cleft secondary palate ( J:268949 )

• cleft secondary palate only is also observed

bilateral cleft palate ( J:268949 )

unilateral cleft palate ( J:268949 )

respiratory system

abnormal external nares morphology ( J:268949 )

• non-cleft presenting embryos exhibit dysmorphic nares

abnormal nasal tip morphology ( J:268949 )

• non-cleft presenting embryos exhibit dysmorphic nasal tips

Genotype
MGI:7345551

cn7

• Allelic Composition: Ctnnd1^tm1Abre/Ctnnd1⁺; Tg(Tcfap2a-cre)1Will/0
• Genetic Background: involves: 129S6/SvEvTac

craniofacial

abnormal maxillary prominence morphology ( J:268949 )

• at E11.5, a delay in the medial growth of the maxillary prominences is observed

• however, no overt clefts are identified

Genotype
MGI:4887452

cn8

• Allelic Composition: Ctnnb1^tm1Mmt/Ctnnb1⁺; Tg(Tcfap2a-cre)1Will/0
• Genetic Background: involves: 129X1/SvJ * C57BL/6

mortality/aging

lethality throughout fetal growth and development, incomplete penetrance ( J:168016 )

• most embryos die between E12.5 and 16.5, likely due to vasculature defects

embryonic lethality during organogenesis, incomplete penetrance ( J:168016 )

• most embryos die between E12.5 and 16.5, likely due to vasculature defects

craniofacial

abnormal Meckel's cartilage morphology ( J:168016 )

• in some mutants this is turned downward and in others, is duplicated on the interior aspect

abnormal mandibular prominence morphology ( J:168016 )

• mandibular prominences is increased in size, resulting in lack of proper lower jaw formation or fusion and forming a widened oral cavity

abnormal maxillary prominence morphology ( J:168016 )

• maxillary prominence is increased in size, resulting in lack of proper upper jaw formation or fusion and forming a widened oral cavity

abnormal medial nasal prominence morphology ( J:168016 )

• the nasal process does not show controlled directional growth that results in formation of a normal nasal pit

abnormal facial morphology ( J:168016 )

• most mutants lack any recognizable facial features; some embryos have discernible features but these show severe defects

abnormal face development ( J:168016 )

• embryos do not exhibit recognizable facial features except for a widened oral cavity

• facial development is normal at E9.0, but soon after facial prominences enlarge more rapidly than wild-type

• no significant changes in cell death or proliferation are detected at E10.5

abnormal oral cavity morphology ( J:168016 )

• widened oral cavity

absent outer ear ( J:168016 )

• at E12.5, mutants lack external (visible) ears

skeleton

abnormal Meckel's cartilage morphology ( J:168016 )

• in some mutants this is turned downward and in others, is duplicated on the interior aspect

abnormal cartilage development ( J:168016 )

• cartilages in the head such as the parachordal plate and cartilages of the ear are grossly malformed

• nasal cartilages are wider and misshapen

ectopic cartilage ( J:168016 )

• mutants have extensive ectopic cartilages in the head region resulting in cartilage fusions and malformations

hearing/vestibular/ear

absent outer ear ( J:168016 )

• at E12.5, mutants lack external (visible) ears

vision/eye

abnormal eye morphology ( J:168016 )

• at E12.5, mutants lack external eyes although rudimentary eyes are found internalized

integument

absent vibrissae ( J:168016 )

• at E12.5, mutants lack vibrissae

growth/size/body

abnormal facial morphology ( J:168016 )

• most mutants lack any recognizable facial features; some embryos have discernible features but these show severe defects

abnormal face development ( J:168016 )

• embryos do not exhibit recognizable facial features except for a widened oral cavity

• facial development is normal at E9.0, but soon after facial prominences enlarge more rapidly than wild-type

• no significant changes in cell death or proliferation are detected at E10.5

abnormal oral cavity morphology ( J:168016 )

• widened oral cavity

absent outer ear ( J:168016 )

• at E12.5, mutants lack external (visible) ears