Phenotypes associated with this allele

• Allele Symbol: Tg(tetO-Zfp521)#Rbar
• Allele Name: transgene insertion, Roland Baron
• Allele ID: MGI:4887924
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Tg(Col1a1-Runx2)#Pmt/0 Tg(Eno2tTA)#Nes/0 Tg(tetO-Zfp521)#Rbar/0	involves: BALB/c * C57BL/6 * SJL	MGI:4887975
cx2	Tg(Eno2tTA)#Nes/0 Tg(tetO-Zfp521)#Rbar/0	involves: C57BL/6 * SJL	MGI:4887972
cx3	Runx2^tm1Mjo/Runx2^+ Tg(Eno2tTA)#Nes/0 Tg(tetO-Zfp521)#Rbar/0	involves: C57BL/6 * SJL	MGI:4887973

Genotype
MGI:4887975

cx1

• Allelic Composition: Tg(Col1a1-Runx2)#Pmt/0; Tg(Eno2tTA)#Nes/0; Tg(tetO-Zfp521)#Rbar/0
• Genetic Background: involves: BALB/c * C57BL/6 * SJL

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

skeleton

skeleton phenotype ( J:167992 )

N • nearly all aspects of the Tg(Col1a1-Runx2)#Pmt phenotype are reversed

decreased compact bone thickness ( J:167992 )

decreased osteocyte number ( J:167992 )

• osteocyte numbers in the cortical bone are reduced compared to in wild-type mice but not as severely as in Tg(Col1a1-Runx2)#Pmt mice

abnormal bone mineralization ( J:167992 )

• mice exhibit reduced cortical mineral apposition rates compared with wild-type mice

fragile skeleton ( J:167992 )

• fracture rate is increased compared to in wild-type mice but not as severely as in Tg(Col1a1-Runx2)#Pmt mice

Genotype
MGI:4887972

cx2

• Allelic Composition: Tg(Eno2tTA)#Nes/0; Tg(tetO-Zfp521)#Rbar/0
• Genetic Background: involves: C57BL/6 * SJL

skeleton

decreased hyoid bone size ( J:167992 )

• the mineralized midpoint of the hyoid bone is decreased in length compared to in wild-type mice

• however, mineralization occurs unlike in Runx2^tm1Mjo heterozygotes

clavicle hypoplasia ( J:167992 )

• clavicle is smaller than in wild-type mice but not as severely reduced as in Runx2^tm1Mjo heterozygotes

increased bone volume ( J:167992 )

• mice exhibit an increase in bone volume compared with wild-type mice

abnormal trabecular bone morphology ( J:167992 )

• mice exhibit an increase in bone volume and trabeculae number and a decrease in trabecular space compared with wild-type mice

increased bone mass ( J:167992 )

• mice exhibit an increase in bone mass compared with wild-type mice

increased bone ossification ( J:167992 )

• mice exhibit increased bone formation compared to in wild-type mice

craniofacial

decreased hyoid bone size ( J:167992 )

• the mineralized midpoint of the hyoid bone is decreased in length compared to in wild-type mice

• however, mineralization occurs unlike in Runx2^tm1Mjo heterozygotes

Genotype
MGI:4887973

cx3

• Allelic Composition: Runx2^tm1Mjo/Runx2⁺; Tg(Eno2tTA)#Nes/0; Tg(tetO-Zfp521)#Rbar/0
• Genetic Background: involves: C57BL/6 * SJL

skeleton

abnormal fontanelle morphology ( J:167992 )

• at P25, mice exhibit a defined nonmineralization area between the anterior and posterior fontanelles compared to in wild-type mice that is larger than in Runx2^tm1Mjo heterozygotes

abnormal hyoid bone morphology ( J:167992 )

• the midportion of the hyoid bone fails to mineralize unlike in wild-type mice

clavicle hypoplasia ( J:167992 )

• to a greater extent than in Runx2^tm1Mjo heterozygotes

craniofacial

abnormal fontanelle morphology ( J:167992 )

• at P25, mice exhibit a defined nonmineralization area between the anterior and posterior fontanelles compared to in wild-type mice that is larger than in Runx2^tm1Mjo heterozygotes

abnormal hyoid bone morphology ( J:167992 )

• the midportion of the hyoid bone fails to mineralize unlike in wild-type mice