Phenotypes associated with this allele

• Allele Symbol: Tg(Lck-cre)19Hhan
• Allele Name: transgene insertion 19, Hua Han
• Allele ID: MGI:4889049
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Rbpj^tm1Hon/Rbpj^tm1Hon Tg(Lck-cre)19Hhan/0	involves: 129P2/OlaHsd * C57BL/6 * DBA	MGI:4889050
cn2	Bsg^tm1.1Znc/Bsg^tm1.1Znc Tg(Lck-cre)19Hhan/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA	MGI:5587948

Genotype
MGI:4889050

cn1

• Allelic Composition: Rbpj^tm1Hon/Rbpj^tm1Hon; Tg(Lck-cre)19Hhan/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * DBA

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

immune system phenotype ( J:168475 )

N • mice show normal T cell development in the thymus and periphery

increased T cell number ( J:168475 )

• engrafted mice show a nearly 2-fold increase in absolute number of T cells, while the B cell compartment is not different from wild-type

increased single-positive T cell number ( J:168475 )

• in draining lymph nodes, a significant increase in CD4+ and CD8+ T cells is detected

increased macrophage cell number ( J:168475 )

• animals exhibit a significant increase in macrophage antigen-1-positive cells in spleens

abnormal CD4-positive helper T cell morphology ( J:168475 )

increased splenocyte number ( J:168475 )

• mice show 1.5-fold increase splenocyte number in engrafted mice

abnormal immune system physiology ( J:168475 )

• skin and cardiac allografts from BALB/c mice to Rbpj-deficient mice show decreased survival time

• grafts of both tissue type show increased leukocyte infiltration

increased T cell proliferation ( J:168475 )

• percentage of proliferating CD4+ cells is significantly increased in lymph nodes and spleen; a similar increase in CD8+ T cells in spleen and lymph nodes is observed in engrafted animals

abnormal regulatory T cell physiology ( J:168475 )

• suppression activity of CD4+CD25+ regulatory T cells is significantly attenuated in vitro; generation of Treg cells in vivo in graft recipients is not altered

hematopoietic system

increased T cell proliferation ( J:168475 )

• percentage of proliferating CD4+ cells is significantly increased in lymph nodes and spleen; a similar increase in CD8+ T cells in spleen and lymph nodes is observed in engrafted animals

increased T cell number ( J:168475 )

• engrafted mice show a nearly 2-fold increase in absolute number of T cells, while the B cell compartment is not different from wild-type

increased single-positive T cell number ( J:168475 )

• in draining lymph nodes, a significant increase in CD4+ and CD8+ T cells is detected

increased macrophage cell number ( J:168475 )

• animals exhibit a significant increase in macrophage antigen-1-positive cells in spleens

abnormal CD4-positive helper T cell morphology ( J:168475 )

increased splenocyte number ( J:168475 )

• mice show 1.5-fold increase splenocyte number in engrafted mice

abnormal regulatory T cell physiology ( J:168475 )

• suppression activity of CD4+CD25+ regulatory T cells is significantly attenuated in vitro; generation of Treg cells in vivo in graft recipients is not altered

cellular

increased T cell proliferation ( J:168475 )

• percentage of proliferating CD4+ cells is significantly increased in lymph nodes and spleen; a similar increase in CD8+ T cells in spleen and lymph nodes is observed in engrafted animals

Genotype
MGI:5587948

cn2

• Allelic Composition: Bsg^tm1.1Znc/Bsg^tm1.1Znc; Tg(Lck-cre)19Hhan/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA

immune system

immune system phenotype ( J:207886 )

N • at 2 and 5 weeks, mice exhibit normal absolute numbers of double negative and CD8 single positive thymocytes and double positive thymocyte apoptosis rates

decreased thymus weight ( J:207886 )

• at 5 weeks

decreased thymocyte number ( J:207886 )

• at 2 and 5 weeks

decreased double-positive T cell number ( J:207886 )

• double positive thymocytes at 2 and 5 weeks

decreased single-positive T cell number ( J:207886 )

• CD4 single positive thymocytes at 2 and 5 weeks

abnormal T cell activation ( J:207886 )

• increased activation in CD4+ T cells stimulated with ConA and anti-CD3/CD28 antibodies

increased T cell proliferation ( J:207886 )

• in CD4+ T cells stimulated with ConA and anti-CD3/CD28 antibodies

• however, PMA/ionomycin-stimulated CD4+ T cells exhibit normal proliferation

endocrine/exocrine glands

decreased thymus weight ( J:207886 )

• at 5 weeks

decreased thymocyte number ( J:207886 )

• at 2 and 5 weeks

hematopoietic system

decreased thymus weight ( J:207886 )

• at 5 weeks

decreased thymocyte number ( J:207886 )

• at 2 and 5 weeks

decreased double-positive T cell number ( J:207886 )

• double positive thymocytes at 2 and 5 weeks

decreased single-positive T cell number ( J:207886 )

• CD4 single positive thymocytes at 2 and 5 weeks

abnormal T cell activation ( J:207886 )

• increased activation in CD4+ T cells stimulated with ConA and anti-CD3/CD28 antibodies

increased T cell proliferation ( J:207886 )

• in CD4+ T cells stimulated with ConA and anti-CD3/CD28 antibodies

• however, PMA/ionomycin-stimulated CD4+ T cells exhibit normal proliferation

cellular

increased T cell proliferation ( J:207886 )

• in CD4+ T cells stimulated with ConA and anti-CD3/CD28 antibodies

• however, PMA/ionomycin-stimulated CD4+ T cells exhibit normal proliferation