Phenotypes associated with this allele

• Allele Symbol: Sting1^gt
• Allele Name: goldenticket
• Allele ID: MGI:4939597
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Sting1^gt/Sting1^gt	C57BL/6J-Sting1^gt/J	MGI:7863519
hm2	Sting1^gt/Sting1^gt	C57BL/6-Sting1^gt	MGI:4939598
hm3	Sting1^gt/Sting1^gt	involves: C57BL/6	MGI:6296747

Genotype
MGI:7863519

hm1

• Allelic Composition: Sting1^gt/Sting1^gt
• Genetic Background: C57BL/6J-Sting1^gt/J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

abnormal interferon-beta secretion ( J:354143 )

• primary bone marrow-derived macrophages (BMDMs) and fibroblasts transfected with 8 ug/mL of interferon stimulatory DNA (ISD) or cGAMP (the activating ligand of STING1) fail to show induction of Ifnb1 (interferon beta 1, fibroblast) mRNA levels, unlike similarly treated wild-type cells

abnormal response to infection ( J:354143 )

• primary BMDMs infected by S. aureus at MOI = 100 show a significant decrease in Ifnb1 mRNA expression relative to similarly infected wild-type BMDMs, with no change in Tnf (tumor necrosis factor) mRNA levels

• however, BMDMs infected by K. pneumoniae show normal Ifnb1 and Tnf mRNA expression

increased susceptibility to Retroviridae infection ( J:354143 )

• newborn pups i.p. injected with wild-type Moloney murine leukemia virus (MLV) or MLV^gGag (a mutant MLV with an unstable capsid) show significantly higher splenic viral titers at 16 dpi than similarly infected wild-type controls or Ifi207^{em1(IMPC)Wtsi} homozygotes

Genotype
MGI:4939598

hm2

• Allelic Composition: Sting1^gt/Sting1^gt
• Genetic Background: C57BL/6-Sting1^gt

mortality/aging

increased susceptibility to Herpesvirales infection induced morbidity/mortality ( J:201167 )

• die within 3 to 4 days after HSV1 infection

immune system

decreased circulating interferon-alpha level ( J:201167 )

• marked reduction in IFN-alpha and IFN-beta in response to HSV1 infection

decreased circulating interferon-beta level ( J:168746 , J:201167 )

• in L. monocytogenes-infected or c-di-GMP-treated mice (J:168746)

• marked reduction in IFN-alpha and IFN-beta in response to HSV1 infection (J:201167)

abnormal humoral immune response ( J:201167 )

• 2'3'cGAMP fails to enhance the production of ovalbumin-specific antibodies on day 17 following injection with ovalbumin

abnormal immune cell physiology ( J:201167 )

• lung fibroblasts transfected with interferon stimulatory DNA fail to produce any detectable level of IFN-beta

• induction of IFN-beta in lung fibroblasts infected with the DNA viruses; herpes simplex virus 1 (HSV1), vaccinia virus (VACV) and a mutant strain of HSV1called d109 is largely abolished

abnormal macrophage physiology ( J:201167 )

• production of IFN-beta and TNF in bone marrow derived macrophages transfected with interferon stimulatory DNA or herring testis DNA are defective

• induction of IFN-beta in l bone marrow derived macrophages infected with VACV or HSV1 strains d109 or 7134 is largely abolished

• induction of IFN-beta in l bone marrow derived macrophages infected with HSV1 is severely but not completely blocked

abnormal dendritic cell physiology ( J:201167 )

• cultured conventional dendritic cells fail to induce IFN-alpha or IFN-beta following transfection with interferon stimulatory DNA or herring testis DNA

• cultured conventional dendritic cells fail to induce IFN-beta following infections with HSV1 strain d109 or VACV and induction by wild-type HSV1 is partially inhibited

abnormal plasmacytoid dendritic cell physiology ( J:201167 )

• induction of IFN-alpha and IFN-beta by interferon stimulatory DNA, poly[dA:dT], and genomic DNA from E. coli and Vibrio cholerae in the presence of liposome is abolished

abnormal response to infection ( J:168746 )

• L. monocytogenes-infected produce less IFN-beta compared with similarly treated wild-type mice

• macrophages exhibit a modest decrease in response to Sendai virus compared with wild-type cells

• however, L. monocytogenes-induced TNFalpha production is normal

increased susceptibility to Herpesvirales infection ( J:201167 )

• marked reduction in IFN-alpha and IFN-beta in response to HSV1 infection

increased susceptibility to Herpesvirales infection induced morbidity/mortality ( J:201167 )

• die within 3 to 4 days after HSV1 infection

homeostasis/metabolism

decreased circulating interferon-alpha level ( J:201167 )

• marked reduction in IFN-alpha and IFN-beta in response to HSV1 infection

decreased circulating interferon-beta level ( J:168746 , J:201167 )

• in L. monocytogenes-infected or c-di-GMP-treated mice (J:168746)

• marked reduction in IFN-alpha and IFN-beta in response to HSV1 infection (J:201167)

hematopoietic system

abnormal macrophage physiology ( J:201167 )

• production of IFN-beta and TNF in bone marrow derived macrophages transfected with interferon stimulatory DNA or herring testis DNA are defective

• induction of IFN-beta in l bone marrow derived macrophages infected with VACV or HSV1 strains d109 or 7134 is largely abolished

• induction of IFN-beta in l bone marrow derived macrophages infected with HSV1 is severely but not completely blocked

Genotype
MGI:6296747

hm3

• Allelic Composition: Sting1^gt/Sting1^gt
• Genetic Background: involves: C57BL/6

digestive/alimentary system

decreased enterocyte apoptosis ( J:269933 )

• intestinal organoids are protected from IL22 and bafilomycin A-induced cell death

hematopoietic system

hematopoietic system phenotype ( J:265023 )

N • normal response by bone marrow-derived macrophages (BMDMs) to LPS, poly(I.C)

cellular

decreased enterocyte apoptosis ( J:269933 )

• intestinal organoids are protected from IL22 and bafilomycin A-induced cell death

immune system

immune system phenotype ( J:265023 )

N • normal response by bone marrow-derived macrophages (BMDMs) to LPS, poly(I.C)

increased susceptibility to Retroviridae infection ( J:265023 )

• lack of interferon beta surge in bone marrow-derived macrophages (BMDMs) and bone marrow dendritic cells (BMDCs) from MLV (murine leukemia virus)-infected mice

• 10x higher virus titers in spleen 16 days after inoculation of newborn pups with MLV (murine leukemia virus)