Analysis Tools
mortality/aging
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• die between E7.5 and E8.5
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embryo
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• arrest at the beginning of gastrulation
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• arrest at the beginning of gastrulation
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• at E5.5, embryos are smaller than littermate controls
• at E7.5 embryos remain the shape of a compact egg and are less than half the size of littermate controls
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• smaller and contain fewer cells at E5.5, E6.5, and E7.5
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• at E6.5 embryos contain less identifiable layers of visceral endoderm and embryonic ectoderm
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• no discernable primitive streak at E6.5
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• at E7.5 a layer of cells that appears to be visceral endoderm is visible but the rest of the embryo is disorganized
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• in the visceral endoderm lipid droplets contain larger vesicles and staining of the droplets is less intense suggesting a defect in the processing or the transportation of internalized lipids and fluids
• at E6.5, embryos contain less identifiable visceral endoderm
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cellular
| N |
• no increase in apoptosis is detected in embryos at E6.5
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• in embryos at E6.5
• cultured blastocysts show reduced cell proliferation and growth between 3 to 8 days in culture
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growth/size/body
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• at E5.5, embryos are smaller than littermate controls
• at E7.5 embryos remain the shape of a compact egg and are less than half the size of littermate controls
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• smaller and contain fewer cells at E5.5, E6.5, and E7.5
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