Phenotypes associated with this allele

• Allele Symbol: Gnao1^tm4Lbi
• Allele Name: targeted mutation 4, Lutz Birnbaumer
• Allele ID: MGI:4941847
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Gnao1^tm4Lbi/Gnao1^tm4Lbi Omp^tm4(cre)Mom/?	involves: 129P2/OlaHsd * C57BL/6J	MGI:6379998
cn2	Gnao1^tm4Lbi/Gnao1^tm4Lbi Tg(Pdx1-cre)89.1Dam/0	involves: C57BL/6 * CBA	MGI:4941850

Genotype
MGI:6379998

cn1

• Allelic Composition: Gnao1^tm4Lbi/Gnao1^tm4Lbi; Omp^tm4(cre)Mom/?
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

abnormal behavior ( J:279957 )

• due to the lack of vomeronasal perception of f-MKKFRW, homozygotes fail to display the normal avoidance behaviour to this core peptide of an enteric bacterial virulence regulator due to the lack of vomeronasal perception of f-MKKFRW, homozygotes fail to display the normal avoidance behaviour to this core peptide of an enteric bacterial virulence regulator due to the lack of vomeronasal perception of f-MKKFRW, homozygotes fail to display the normal avoidance behaviour to this core peptide of an enteric bacterial virulence regulator ue to the lack of vomeronasal perception of f-MKKFRW, homozygotes fail to display the normal avoidance behaviour to this core peptide of an enteric bacterial virulence regulator

nervous system

abnormal vomeronasal sensory neuron physiology ( J:279957 )

• fail to respond to the f-MKKFRW peptide, which is the core peptide motif from the enteric bacterial master virulence regulator MgrB

Genotype
MGI:4941850

cn2

• Allelic Composition: Gnao1^tm4Lbi/Gnao1^tm4Lbi; Tg(Pdx1-cre)89.1Dam/0
• Genetic Background: involves: C57BL/6 * CBA

endocrine/exocrine glands

abnormal pancreas morphology ( J:169627 )

• at P28 and P56, pancreatic insulin content is reduced compared to in wild-type mice

• however, insulin content at P1 is normal

decreased pancreatic beta cell mass ( J:169627 )

• at P56

abnormal pancreatic beta cell physiology ( J:169627 )

• unstimulated and glucose-stimulated beta cells increased docking and release of secretory vesicles compared with wild-type cells

• however, the total number of vesicles is normal

increased insulin secretion ( J:169627 )

• in stimulated pancreatic islet cells

homeostasis/metabolism

increased insulin secretion ( J:169627 )

• in stimulated pancreatic islet cells

increased circulating insulin level ( J:169627 )

• after glucose challenge

abnormal glucose tolerance ( J:169627 )

• in an intraperitoneal glucose tolerance test, mice exhibit improved glucose clearance compared with wild-type mice

• pertussis toxin-treated mice fail to exhibit an increase in glucose tolerance unlike similarly treated wild-type mice

• however, insulin sensitivity is normal