Phenotypes associated with this allele

• Allele Symbol: Lrig1^{tm1.1(cre/ERT2)Rjc}
• Allele Name: targeted mutation 1.1, Robert J Coffey
• Allele ID: MGI:4947937
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Lrig1^tm1.1(cre/ERT2)Rjc/Lrig1^tm1.1(cre/ERT2)Rjc	involves: 129 * C57BL/6	MGI:5432134
cn2	Scd1^tm2Ntam/Scd1^tm2Ntam Lrig1^tm1.1(cre/ERT2)Rjc/Lrig1^+	B6.129(Cg)-Lrig1^tm1.1(cre/ERT2)Rjc Scd1^tm2Ntam	MGI:7287840
cn3	Apc^tm2.1Cip/Apc^+ Lrig1^tm1.1(cre/ERT2)Rjc/Lrig1^+	involves: 129 * 129P2/OlaHsd * C57BL/6	MGI:5432136
cx4	Lgr5^tm1(cre/ERT2)Cle/Lgr5^+ Lrig1^tm1.1(cre/ERT2)Rjc/Lrig1^tm1.1(cre/ERT2)Rjc	involves: 129 * 129P2/OlaHsd * C57BL/6	MGI:5432135

Genotype
MGI:5432134

hm1

• Allelic Composition: Lrig1^{tm1.1(cre/ERT2)Rjc}/Lrig1^{tm1.1(cre/ERT2)Rjc}
• Genetic Background: involves: 129 * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

normal phenotype

no abnormal phenotype detected ( J:186084 )

• homozygotes (and heterozygotes) are viable and fertile

Genotype
MGI:7287840

cn2

• Allelic Composition: Scd1^tm2Ntam/Scd1^tm2Ntam; Lrig1^{tm1.1(cre/ERT2)Rjc}/Lrig1⁺
• Genetic Background: B6.129(Cg)-Lrig1^{tm1.1(cre/ERT2)Rjc} Scd1^tm2Ntam

integument

abnormal hair shaft morphology ( J:325761 )

• decreased hair shaft

abnormal hair follicle matrix region morphology ( J:325761 )

• loss of matrix progenitors

decreased hair follicle number ( J:325761 )

hair follicle degeneration ( J:325761 )

abnormal dermis papillary layer morphology ( J:325761 )

• loss of dermal papilla cells

Genotype
MGI:5432136

cn3

• Allelic Composition: Apc^tm2.1Cip/Apc⁺; Lrig1^{tm1.1(cre/ERT2)Rjc}/Lrig1⁺
• Genetic Background: involves: 129 * 129P2/OlaHsd * C57BL/6

neoplasm

increased intestinal adenoma incidence ( J:186084 )

• with tamoxifen treatement, colon tumors are observed by day 50 following Apc^fldeletion with cre induction (and stochastic loss of second Apc allele)

• by day 50 there are multiple distal colonic tumors with no proximal colon tumor burden; tumor phenotype is 100% penetrant

• largest tumors are found in distal colon, many having high grade dysplasia; highest tumor number is observed in jejunum

digestive/alimentary system

increased intestinal adenoma incidence ( J:186084 )

• with tamoxifen treatement, colon tumors are observed by day 50 following Apc^fldeletion with cre induction (and stochastic loss of second Apc allele)

• by day 50 there are multiple distal colonic tumors with no proximal colon tumor burden; tumor phenotype is 100% penetrant

• largest tumors are found in distal colon, many having high grade dysplasia; highest tumor number is observed in jejunum

Genotype
MGI:5432135

cx4

• Allelic Composition: Lgr5^{tm1(cre/ERT2)Cle}/Lgr5⁺; Lrig1^{tm1.1(cre/ERT2)Rjc}/Lrig1^{tm1.1(cre/ERT2)Rjc}
• Genetic Background: involves: 129 * 129P2/OlaHsd * C57BL/6

neoplasm

increased intestinal adenoma incidence ( J:186084 )

• at 5-6 months, nearly 90% of mice develop duodenal tumors (usually low grade adenomas)

• in one instance, a 13-month old mouse had a tumor with areas showing high grade dysplasia, and focal extension of neoplastic glands into deeper layers of the bowel wall; tumor showed intact Apc however

digestive/alimentary system

abnormal crypts of Lieberkuhn morphology ( J:186084 )

• number of crypts showing Lgr5-EGFP fluorescence is doubled in absence of Lrig1 protein, compared to mice with one or two copies of Lrig1

increased intestinal adenoma incidence ( J:186084 )

• at 5-6 months, nearly 90% of mice develop duodenal tumors (usually low grade adenomas)

• in one instance, a 13-month old mouse had a tumor with areas showing high grade dysplasia, and focal extension of neoplastic glands into deeper layers of the bowel wall; tumor showed intact Apc however

endocrine/exocrine glands

abnormal crypts of Lieberkuhn morphology ( J:186084 )

• number of crypts showing Lgr5-EGFP fluorescence is doubled in absence of Lrig1 protein, compared to mice with one or two copies of Lrig1