Phenotypes associated with this allele

• Allele Symbol: Kcnu1^tm1.2Clin
• Allele Name: targeted mutation 1.2, Christopher J Lingle
• Allele ID: MGI:4949763
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Kcnu1^tm1.2Clin/Kcnu1^tm1.2Clin	B6.Cg-Kcnu1^tm1.2Clin	MGI:4949840
hm2	Kcnu1^tm1.2Clin/Kcnu1^tm1.2Clin	involves: C57BL/6NTac	MGI:5896619
ht3	Kcnu1^tm1.2Clin/Kcnu1^+	B6.Cg-Kcnu1^tm1.2Clin	MGI:4949841

Genotype
MGI:4949840

hm1

• Allelic Composition: Kcnu1^tm1.2Clin/Kcnu1^tm1.2Clin
• Genetic Background: B6.Cg-Kcnu1^tm1.2Clin

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

reproductive system

male infertility ( J:171225 )

♂

impaired fertilization ( J:171225 )

♂ • capacitated sperm exhibits reduced in vitro fertilization of oocytes compared with wild-type sperm

abnormal sperm physiology ( J:171225 )

♂ • nearly all alkalization-activated potassium ion current is abolished in spermatozoa unlike in wild-type cells

♂ • spermatozoa lack alkalization-mediated hyperpolarization unlike wild-type cells

♂ • sperm osmoregulation is disrupted compared to in wild-type cells

♂ • however, mice exhibit normal residual pH-dependent outward potassium current

asthenozoospermia ( J:171225 )

♂

cellular

asthenozoospermia ( J:171225 )

♂

Genotype
MGI:5896619

hm2

• Allelic Composition: Kcnu1^tm1.2Clin/Kcnu1^tm1.2Clin
• Genetic Background: involves: C57BL/6NTac

reproductive system

impaired fertilization ( J:220171 )

♂ • IVF success is markedly reduced

abnormal sperm physiology ( J:220171 )

♂ • decreased current variance in corpus epididymal sperm for alkaline-dependent currents activated by 40-ms voltage steps from -100 to +200 mV

♂ • activation is only detected starting at +100mV unlike in controls

♂ • impaired alkaline pH induced hyperpolarization

Genotype
MGI:4949841

ht3

• Allelic Composition: Kcnu1^tm1.2Clin/Kcnu1⁺
• Genetic Background: B6.Cg-Kcnu1^tm1.2Clin

reproductive system

reproductive system phenotype ( J:171225 )

N • mice exhibit normal fertility

abnormal sperm physiology ( J:171225 )

♂ • some alkalization-activated potassium ion current is abolished in spermatozoa unlike in wild-type cells