Phenotypes associated with this allele

• Allele Symbol: Grb2^tm1Lnit
• Allele Name: targeted mutation 1, Lars Nitschke
• Allele ID: MGI:4949889
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Grb2^tm1Lnit/Grb2^tm1Lnit Cd79a^tm1(cre)Reth/Cd79a^+	involves: BALB/c * BALB/cJ	MGI:4949890

Genotype
MGI:4949890

cn1

• Allelic Composition: Grb2^tm1Lnit/Grb2^tm1Lnit; Cd79a^tm1(cre)Reth/Cd79a⁺
• Genetic Background: involves: BALB/c * BALB/cJ

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

abnormal B cell differentiation ( J:171222 )

• in an adoptive transfer experiment, B cells are at a competitive disadvantage to wild-type B cells

decreased transitional stage T1 B cell number ( J:171222 )

decreased transitional stage T2 B cell number ( J:171222 )

increased plasma cell number ( J:171222 )

• IgM-secreting plasma cells

decreased B cell number ( J:171222 )

• in peripheral blood

decreased mature B cell number ( J:171222 )

• mice exhibit a reduction in recirculating, mature (IgM+B220^hi fraction F) B cells in the bone marrow compared with Grb2^tm1Lnit homozygote controls

• the number of mature B cells in the spleen is half that in wild-type mice

• however, mice exhibit normal numbers of marginal zone B cells and B1 cells in the spleen and peritoneal cavity

decreased follicular B cell number ( J:171222 )

decreased spleen germinal center number ( J:171222 )

• in mice immunized with sheep red blood cell

decreased spleen germinal center size ( J:171222 )

• in mice immunized with sheep red blood cell

abnormal B cell physiology ( J:171222 )

• mice exhibit reduced Brd-U labeled T1/marginal zone B cells compared with cells from Grb2^tm1Lnit homozygote controls

• however, bone marrow pre-B and immature B cell proliferation is normal

increased B cell apoptosis ( J:171222 )

• immature and mature B cells exhibit a slight increase in spontaneous apoptosis compared to in Grb2^tm1Lnit homozygote controls

increased B cell proliferation ( J:171222 )

• after anti-IgM or LPS stimulation

decreased IgG level ( J:171222 )

• in response to secondary exposure to human cytomegalovirus-derived virus-like particles

• however, primary IgG response is normal

decreased IgG3 level ( J:171222 )

• in TNP-ficoll treated mice

increased IgM level ( J:171222 )

• 10-fold in unstimulated mice

• however, IgM response to TNP-ficoll is normal

abnormal memory B cell physiology ( J:171222 )

• mice exhibit impaired memory response to human cytomegalovirus-derived virus-like particles compared with Grb2^tm1Lnit homozygote controls

hematopoietic system

abnormal B cell differentiation ( J:171222 )

• in an adoptive transfer experiment, B cells are at a competitive disadvantage to wild-type B cells

decreased transitional stage T1 B cell number ( J:171222 )

decreased transitional stage T2 B cell number ( J:171222 )

increased plasma cell number ( J:171222 )

• IgM-secreting plasma cells

decreased B cell number ( J:171222 )

• in peripheral blood

decreased mature B cell number ( J:171222 )

• mice exhibit a reduction in recirculating, mature (IgM+B220^hi fraction F) B cells in the bone marrow compared with Grb2^tm1Lnit homozygote controls

• the number of mature B cells in the spleen is half that in wild-type mice

• however, mice exhibit normal numbers of marginal zone B cells and B1 cells in the spleen and peritoneal cavity

decreased follicular B cell number ( J:171222 )

decreased spleen germinal center number ( J:171222 )

• in mice immunized with sheep red blood cell

decreased spleen germinal center size ( J:171222 )

• in mice immunized with sheep red blood cell

abnormal B cell physiology ( J:171222 )

• mice exhibit reduced Brd-U labeled T1/marginal zone B cells compared with cells from Grb2^tm1Lnit homozygote controls

• however, bone marrow pre-B and immature B cell proliferation is normal

increased B cell apoptosis ( J:171222 )

• immature and mature B cells exhibit a slight increase in spontaneous apoptosis compared to in Grb2^tm1Lnit homozygote controls

increased B cell proliferation ( J:171222 )

• after anti-IgM or LPS stimulation

decreased IgG level ( J:171222 )

• in response to secondary exposure to human cytomegalovirus-derived virus-like particles

• however, primary IgG response is normal

decreased IgG3 level ( J:171222 )

• in TNP-ficoll treated mice

increased IgM level ( J:171222 )

• 10-fold in unstimulated mice

• however, IgM response to TNP-ficoll is normal

abnormal memory B cell physiology ( J:171222 )

• mice exhibit impaired memory response to human cytomegalovirus-derived virus-like particles compared with Grb2^tm1Lnit homozygote controls

cellular

increased B cell apoptosis ( J:171222 )

• immature and mature B cells exhibit a slight increase in spontaneous apoptosis compared to in Grb2^tm1Lnit homozygote controls

increased B cell proliferation ( J:171222 )

• after anti-IgM or LPS stimulation