Phenotypes associated with this allele

• Allele Symbol: Neat1^tm1.1Shna
• Allele Name: targeted mutation 1.1, Shinichi Nakagawa
• Allele ID: MGI:4949933
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Neat1^tm1.1Shna/Neat1^tm1.1Shna	involves: 129S4/SvJaeSor * C57BL/6Ncrlj * CBA/JNCrlj	MGI:4949934
hm2	Neat1^tm1.1Shna/Neat1^tm1.1Shna	involves: C57BL/6NCrlj * CBA/JNCrlj	MGI:5705314

Genotype
MGI:4949934

hm1

• Allelic Composition: Neat1^tm1.1Shna/Neat1^tm1.1Shna
• Genetic Background: involves: 129S4/SvJaeSor * C57BL/6Ncrlj * CBA/JNCrlj

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cellular

abnormal cell nucleus morphology ( J:171281 )

• disruption of paraspeckle formation in MEFs and in esophageal epithelial cells, forestomach epithelial cells, and surface mucous cells in zymogenic stomach all of which express high levels of the long transcript in wild-type mice

mortality/aging

mortality/aging ( J:171281 )

N • despite disruption of paraspeckle formation, mice are viable and fertile

digestive/alimentary system

digestive/alimentary phenotype ( J:171281 )

N • despite disruption of paraspeckle formation, gross stomach histological organization is normal

Genotype
MGI:5705314

hm2

• Allelic Composition: Neat1^tm1.1Shna/Neat1^tm1.1Shna
• Genetic Background: involves: C57BL/6NCrlj * CBA/JNCrlj

integument

decreased mammary gland epithelial cell proliferation ( J:228123 )

♀ • at mid-gestation (8.5 and 12.5 days post-coitum)

♀ • however, proliferation at the start of pregnancy is normal

abnormal mammary gland morphology ( J:228123 )

♀ • paraspeckles are absent in mammary glands of virgin mice

abnormal mammary gland development ( J:228123 )

♀ • impaired

abnormal mammary gland epithelium morphology ( J:228123 )

♀ • reduced milk-producing epithelial cell number at day 2 of lactation

♀ • however, cell size is normal

abnormal branching of the mammary ductal tree ( J:228123 )

♀ • reduced branching at all virgin adult time points

♀ • abnormal branching morphogenesis and aberrant ductal extension at early stages of puberty

♀ • decreased density of ducts by the end of puberty with reduced number of secondary branch points and increased interbranch distance

♀ • however, primary and secondary ducts reach the distal edge of the fat pad

abnormal mammary gland alveolus morphology ( J:228123 )

♀ • fewer alveoli in lactating dams with reduced milk-producing epithelial cell number at day 2 of lactation

abnormal lactation ( J:228123 )

♀ • partial loss of cytoplasmic droplets indicative of active milk secretion

♀ • however, mice exhibit normal milk production and secretion

growth/size/body

decreased body weight ( J:228123 )

• in pups at 3.5 and 6 weeks

• however, pups fostered by wild-type dams exhibit normal weight gain

behavior/neurological

decreased food intake ( J:228123 )

• pups that survive beyond 5 days after birth have smaller amounts of milk in their stomachs compared with wild-type mice

cellular

decreased mammary gland epithelial cell proliferation ( J:228123 )

♀ • at mid-gestation (8.5 and 12.5 days post-coitum)

♀ • however, proliferation at the start of pregnancy is normal

maternal effect ( J:228123 )

• a minority of pups survive beyond day 5 after birth and exhibit reduced body weight

• however, pups fostered by wild-type dams exhibit normal survival and body weight

endocrine/exocrine glands

decreased mammary gland epithelial cell proliferation ( J:228123 )

♀ • at mid-gestation (8.5 and 12.5 days post-coitum)

♀ • however, proliferation at the start of pregnancy is normal

abnormal mammary gland morphology ( J:228123 )

♀ • paraspeckles are absent in mammary glands of virgin mice

abnormal mammary gland development ( J:228123 )

♀ • impaired

abnormal mammary gland epithelium morphology ( J:228123 )

♀ • reduced milk-producing epithelial cell number at day 2 of lactation

♀ • however, cell size is normal

abnormal branching of the mammary ductal tree ( J:228123 )

♀ • reduced branching at all virgin adult time points

♀ • abnormal branching morphogenesis and aberrant ductal extension at early stages of puberty

♀ • decreased density of ducts by the end of puberty with reduced number of secondary branch points and increased interbranch distance

♀ • however, primary and secondary ducts reach the distal edge of the fat pad

abnormal mammary gland alveolus morphology ( J:228123 )

♀ • fewer alveoli in lactating dams with reduced milk-producing epithelial cell number at day 2 of lactation

abnormal lactation ( J:228123 )

♀ • partial loss of cytoplasmic droplets indicative of active milk secretion

♀ • however, mice exhibit normal milk production and secretion